Analysis of the Main Components and Antioxidant Activity of Royal Jelly in Different Flowering Periods

YIN Xin; QIAO Dong; LI Hongxia; LIU Zhaolong; WU Meijia; PANG Jie; CHEN Lanzhen

doi:10.13386/j.issn1002-0306.2021110228

Volume 43 Issue 17

Aug. 2022

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Science and Technology of Food Industry > 2022 > 43(17): 291-297. > DOI: 10.13386/j.issn1002-0306.2021110228

YIN Xin, QIAO Dong, LI Hongxia, et al. Analysis of the Main Components and Antioxidant Activity of Royal Jelly in Different Flowering Periods[J]. Science and Technology of Food Industry, 2022, 43(17): 291−297. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110228.

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Analysis of the Main Components and Antioxidant Activity of Royal Jelly in Different Flowering Periods

YIN Xin^{1, 2,},
QIAO Dong^{1, 2},
LI Hongxia²,
LIU Zhaolong²,
WU Meijia²,
PANG Jie^1, ,,
CHEN Lanzhen^2, ,

1.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2.
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China

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Received Date: November 18, 2021
Available Online: July 04, 2022

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Abstract

Abstract

The aim of the present study was to explore the differences of quality and antioxidant activity of royal jelly in different flowering periods. The contents of 10-hydroxy-2-decenoic acid (10-HDA), total protein, moisture, and total phenolic acid in royal jelly from the same conditions were analyzed, and the in vitro antioxidant activities were also compared by DPPH method and FRAP method. The results showed that there were significant differences (P<0.05) in 10-HDA contents of royal jelly during different flowering periods, and the content of 10-HDA was the highest in grape royal jelly. The contents of total protein in royal jelly ranged from 13.58% to 15.26%. The contents of moisture in royal jelly were lower than 67.5%, which met the requirements of national standard (GB 9697-2008) regarding to the moisture content of premium royal jelly. Compared with other royal jelly at flowering periods, the total phenolic acid content, DPPH radical scavenging ability and total antioxidant capacity of Vitex royal jelly were the best. And the DPPH free radical scavenging rates of royal jelly at different flowering periods were significantly different (P<0.05). The correlation analysis results showed that the DPPH radical scavenging ability was significantly correlated with the total phenolic acid and the total antioxidant ability (P<0.01).
- royal jelly,
- different flowering periods,
- 10-hydroxy-2-decenoic acid,
- antioxidant,
- scavenging free radicals,
- total phenolic acids

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References (38)

References

[1]	FRATINI F, CILIA G, MANCINI S, et al. Royal jelly: An ancient remedy with remarkable antibacterial properties[J]. Microbiological Research,2016,192:130−141. doi: 10.1016/j.micres.2016.06.007
[2]	苏晔, 敬璞, 丁晓雯, 等. 蜂王浆的化学成分生理活性及应用[J]. 农牧产品开发,2000(7):11−12. [SU Y, JING P, DING X W, et al. The physiological activity and application of the chemical components of royal jelly[J]. Agriculture Products Development,2000(7):11−12. SU Y, JING P, DING X W, et al. The physiological activity and application of the chemical components of royal jelly [J]. Agriculture Products Development, 2000 (7): 11-12.
[3]	邵琪琪, 林焱, 张以宏, 等. 蜂王浆中活性成分的药理作用研究进展[J]. 食品工业,2018,39(4):276−279. [SHAO Q Q, LIN Y, ZHANG Y H, et al. The research progress on pharmacological effects of active components in royal jelly[J]. The Food Industry,2018,39(4):276−279. SHAO Q Q, LIN Y, ZHANG Y H, et al. The research progress on pharmacological effects of active components in royal jelly[J]. The Food Industry, 2018, 39(4): 276-279.
[4]	PARK M J, KIM B Y, PARK H G, et al. Major royal jelly protein 2 acts as an antimicrobial agent and antioxidant in royal jelly[J]. Journal of Asia-Pacific Entomology,2019,22(3):684−689. doi: 10.1016/j.aspen.2019.05.003
[5]	KOKOTOU M G, MANTZOURANI C, BABAITI R, et al. Study of the royal jelly free fatty acids by liquid chromatography-high resolution mass spectrometry (LC-HRMS)[J]. Metabolites,2020,10(1):40. doi: 10.3390/metabo10010040
[6]	XUE X, WU L, WANG K. Chemical composition of royal jelly[M]. Bee Products-chemical and Biological Properties Springer, 2017: 181−190.
[7]	OKAMOTO I, TANIGUCHI Y, KUNIKATA T, et al. Major royal jelly protein 3 modulates immune responses in vitro and in vivo[J]. Life Sciences,2003,73(16):2029−2045. doi: 10.1016/S0024-3205(03)00562-9
[8]	KUNUGI H, MOHAMMED ALI A. Royal jelly and its components promote healthy aging and longevity: From animal models to humans[J]. International Journal of Molecular Sciences,2019,20(19):4662.
[9]	CHI X P, LIU Z G, WEI W, et al. Selenium-rich royal jelly inhibits hepatocellular carcinoma through PI3K/AKT and VEGF pathways in H22 tumor-bearing mice[J]. Food & Function,2021,12(19):9111−9127.
[10]	ZHANG S, SHAO Q Q, GENG H Y, et al. The effect of royal jelly on the growth of breast cancer in mice[J]. Oncology Letters,2017,14(6):7615−7621.
[11]	BAYRAM N E, CEBI N, CELIK S, et al. Turkish royal jelly: Amino acid, physicochemical, antioxidant, multi-elemental, antibacterial and fingerprint profiles by analytical techniques combined with chemometrics[J]. Journal of Apicultural Research,2021,60(5):751−764. doi: 10.1080/00218839.2021.1889222
[12]	杨远帆, 叶兴乾, 倪辉, 等. 蜂王浆抗菌作用的研究进展[J]. 食品科学,2007,28(11):611−614. [YANG Y F, YE X Q, NI H, et al. Research progress on the antibacterial rffect of royal jelly[J]. Food Science,2007,28(11):611−614. doi: 10.3321/j.issn:1002-6630.2007.11.149 YANG Y F, YE X Q, NI H, et al. Research progress on the antibacterial rffect of royal jelly [J]. Food Science, 2007, 28(11): 611-614. doi: 10.3321/j.issn:1002-6630.2007.11.149
[13]	RAMANATHAN A N K G, NAIR A J, SUGUNAN V S. A review on royal jelly proteins and peptides[J]. Journal of Functional Foods,2018,44:255−264. doi: 10.1016/j.jff.2018.03.008
[14]	PAN Y, RONG Y, YOU M, et al. Royal jelly causes hypotension and vasodilation induced by increasing nitric oxide production[J]. Food Science & Nutrition,2019,7(4):1361−1370.
[15]	刘一冰, 吴德群, 蔺哲广, 等. 蜂王浆生物学功能研究进展[J]. 畜牧兽医学报,2021,52(6):1498−1510. [LIU Y B, WU D Q, LIN Z G, et al. Review on biological function of royal jelly[J]. Acta Veterinaria Et Zootechnica Sinica,2021,52(6):1498−1510. doi: 10.11843/j.issn.0366-6964.2021.06.006 LIU Y B, WU D Q, LIN Z G, et al. Review on biological function of royal jelly[J]. Acta Veterinaria Et Zootechnica Sinica, 2021, 52(6): 1498-1510. doi: 10.11843/j.issn.0366-6964.2021.06.006
[16]	LIU J R, YANG Y C, SHI L S, et al. Antioxidant properties of royal jelly associated with larval age and time of harvest[J]. J Agric Food Chem,2008,56(23):11447−11452. doi: 10.1021/jf802494e
[17]	谌迪, 肖朝耿, 盛玲冬, 等. 不同采收时期蜂王浆抗氧化活性研究[J]. 核农学报,2020,34(7):1491−1496. [CHEN D, XIAO C G, SHENG L D, et al. Antioxidant activity of royal jelly in different collection times[J]. Journal of Nuclear Agricultural Sciences,2020,34(7):1491−1496. doi: 10.11869/j.issn.100-8551.2020.07.1491 CHEN D, XIAO C G, SHENG L D, et al. Antioxidant activity of royal jelly in different collection times[J]. Journal of Nuclear Agricultural Sciences, 2020, 34(7): 1491-1496. doi: 10.11869/j.issn.100-8551.2020.07.1491
[18]	CHEN Y F, YOU M M, LIU Y C, et al. Potential protective effect of Trans-10-hydroxy-2-decenoic acid on the inflammation induced by lipoteichoic acid[J]. Journal of Functional Foods,2018,45:491−498. doi: 10.1016/j.jff.2018.03.029
[19]	YANG Y C, CHOU W M, WIDOWATI D A, et al. 10-hydroxy-2-decenoic acid of royal jelly exhibits bactericide and anti-inflammatory activity in human colon cancer cells[J]. BMC Complement Altern Med,2018,18(1):202. doi: 10.1186/s12906-018-2267-9
[20]	KOCOT J, KIELCZYKOWSKA M, LUCHOWSKA-KOCOT D, et al. Antioxidant potential of propolis, bee pollen, and royal jelly: Possible medical application[J]. Oxidative Medicine and Cellular Longevity,2018,2018:7074209.
[21]	杨相甫, 韩书亮, 李发启, 等. 华北蜜粉源植物的研究[J]. 广西植物,2005,25(1):33−39. [YANG X F, HAN S L, LI F Q, et al. A study on the nectar and pollen plants in North China[J]. A study on the Nectar and Pollen Plants in North China,2005,25(1):33−39. doi: 10.3969/j.issn.1000-3142.2005.01.007 YANG X F, HAN S L, LI F Q, et al. A study on the nectar and pollen plants in North China[J]. A study on the Nectar and Pollen Plants in North China, 2005, 25(1): 33-39. doi: 10.3969/j.issn.1000-3142.2005.01.007
[22]	胡元强, 周萍. 转地蜂场生产蜂王浆10-HDA含量调查研究[J]. 蜜蜂杂志,2020,40(5):2−7. [HU Y Q, ZHOU P. Investigation and research on 10-HDA content of royal jelly produced in transitional apiary[J]. Journal of Bee,2020,40(5):2−7. Hu Y Q, Zhou P. Investigation and Research on 10-HDA content of royal jelly produced in transitional apiary [J]. Journal of Bee, 2020, 40(5) : 2-7.
[23]	中国国家标准化管理委员会. GB 9697-2008 蜂王浆 [S]. 北京: 中国标准出版社, 2008. China National Standardization Administration. GB 9697-2008 Royal jelly [S]. Beijing: China Standards Press, 2008.
[24]	TUBEROSO C I G, ROSA A, BIFULCO E, et al. Chemical composition and antioxidant activities of Myrtus communis L. berries extracts[J]. Food Chemistry,2010,123(4):1242−1251. doi: 10.1016/j.foodchem.2010.05.094
[25]	高慧, 程妮, 贾琪, 等. 蜂王浆冻干粉体外抗氧化作用[J]. 食品科学,2011,32(21):52−55. [GAO H, CHENG N, JIA Q, et al. In vitro antioxidant activity of lyophilized rape royal jelly[J]. Food Science,2011,32(21):52−55. GAO H, CHENG N, JIA Q, et al. In vitro antioxidant activity of lyophilized rape royal jelly[J]. Food Science, 2011, 32(21): 52-55.
[26]	付中民, 李益武, 缪晓青. 不同水解度蜂王浆蛋白体外抗氧化活性的分析[J]. 食品科技,2012(9):54−57. [FU Z M, LI Y W, MIAO X Q. Analysis of antioxidant activity of different hydrolysis degree of royal jelly proteins in vitro[J]. Food Science and Technology,2012(9):54−57. FU Z M, LI Y W, MIAO X Q. Analysis of antioxidant activity of different hydrolysis degree of royal jelly proteins in vitro[J]. Food Science and Technology, 2012 (9): 54-57.
[27]	PAVEL C I, MARGHITAS L A, DEZMIREAN D S, et al. Comparison between local and commercial royal jelly-use of antioxidant activity and 10-hydroxy-2-decenoic acid as quality parameter[J]. Journal of Apicultural Research,2014,53(1):116−123. doi: 10.3896/IBRA.1.53.1.12
[28]	PARK M J, KIM B Y, DENG Y J, et al. Antioxidant capacity of major royal jelly proteins of honeybee (Apis mellifera) royal jelly[J]. Journal of Asia-Pacific Entomology,2020,23(2):445−448. doi: 10.1016/j.aspen.2020.03.007
[29]	SHIMADA K, FUJIKAWA K, YAHARA K, et al. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion[J]. Journal of Agricultural and Food Chemistry,1992,40(6):945−948. doi: 10.1021/jf00018a005
[30]	朱红娟. 贮藏对蜂王浆的品质、抗氧化活性以及美拉德褐变的影响[D]. 西安: 西北大学, 2013. ZHU H J. Effects of storage on the quality, antioxidant activity and Maillard browning of royal jelly [D]. Xi 'an: Northwest University, 2013.
[31]	BENZIE I F, STRAIN J J. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay[J]. Anal Biochem,1996,239(1):70−76. doi: 10.1006/abio.1996.0292
[32]	缪卓宁, 胡福良. 蜂王浆特有成分10-HDA的研究进展[J]. 蜜蜂杂志,2020,40(2):4−9. [MIAO Z N, HU F L. Research progress in the unique component 10-HDA in royal jelly[J]. Journal of Bee,2020,40(2):4−9. MIAO Z N, HU F L. Research progress in the unique component 10-HDA in royal jelly[J]. Journal of Bee, 2020, 40(2): 4-9.
[33]	王艳辉, 余玉生, 卢焕仙, 等. 不同品种蜜蜂及不同花期生产蜂王浆癸烯酸含量的差异[J]. 江苏农业科学,2012,40(12):337−338. [WANG Y H, YU Y S, LU H X, et al. The difference of decenoic acid content in royal jelly produced by different species of bees and different flowering periods[J]. Jiangsu Agricultural Sciences,2012,40(12):337−338. doi: 10.3969/j.issn.1002-1302.2012.12.135 WANG Y H, YU Y S, LU H X, et al. The difference of decenoic acid content in royal jelly produced by different species of bees and different flowering periods [J]. Jiangsu Agricultural Sciences, 2012, 40(12): 337-338. doi: 10.3969/j.issn.1002-1302.2012.12.135
[34]	李肖. 饲料蛋白源对意大利蜜蜂繁蜂效果及蜂王浆品质的影响[D]. 泰安: 山东农业大学. 2014. LI X. The effect of feed protein source on the reproduction effect of Italian bees and the quality of royal jelly [D]. Taian: Shandong Agricultural University, 2014.
[35]	路璐, 李露, 庞倩, 等. 不同糖饲料对蜂王浆主要成分含量的影响[J]. 中国蜂业,2015,66(10):21−23. [LU L, LI L, PANG Q, et al. Effects of different sugar diets on the main components of royal jelly[J]. Apiculture of China,2015,66(10):21−23. doi: 10.3969/j.issn.0412-4367.2015.10.011 LU L, LI L, PANG Q, et al. Effects of different sugar diets on the main components of royal jelly[J]. Apiculture of China, 2015, 66(10): 21-23. doi: 10.3969/j.issn.0412-4367.2015.10.011
[36]	胡元强. 西方蜜蜂生产蜂王浆中10-HDA含量调查研究[J]. 蜜蜂杂志,2016,36(9):5−8. [HU Y Q. Investigation on 10-HDA content in royal jelly produced by western bees[J]. Journal of Bee,2016,36(9):5−8. doi: 10.3969/j.issn.1003-9139.2016.09.002 HU Y Q. Investigation on 10-HDA content in royal jelly produced by western bees [J]. Journal of Bee, 2016, 36(9): 5-8. doi: 10.3969/j.issn.1003-9139.2016.09.002
[37]	敖塘堰, 熊亮, 马振刚. 中华蜜蜂与意大利蜜蜂访花行为特点与区别[J]. 蜜蜂杂志,2021,41(3):1−4. [AO T Y, XIONG L, MA Z G. Review on the characteristics and differences of visiting behavior between honeybee apis cerana and apis mellifera[J]. Journal of Bee,2021,41(3):1−4. AO T Y, XIONG L, MA Z G. Review on the characteristics and differences of visiting behavior between honeybee apis cerana and apis mellifera[J]. Journal of Bee, 2021, 41(3): 1-4.
[38]	罗建能. 中华蜜蜂与意大利蜜蜂温室授粉行为与效果的研究[D]. 杭州: 浙江大学, 2008. LUO J N. Study on pollination behavior and effect of Chinese honeybee and Italian honeybee in greenhouse[D]. Hangzhou: Zhejiang University, 2008.