Purification and Composition of Polysaccharides from <i>Pseudostellaria heterophylla</i> and Protective Effect on Raw 264.7 Cells Induced by LPS

XIE Meilin; XU Haiqing; LI Jingming; ZHA Daoxi; WANG Xueying; CHEN Yang; ZHU Qifa

doi:10.13386/j.issn1002-0306.2021100292

Volume 43 Issue 14

Jul. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(14): 392-400. > DOI: 10.13386/j.issn1002-0306.2021100292

XIE Meilin, XU Haiqing, LI Jingming, et al. Purification and Composition of Polysaccharides from Pseudostellaria heterophylla and Protective Effect on Raw 264.7 Cells Induced by LPS[J]. Science and Technology of Food Industry, 2022, 43(14): 392−400. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100292.

Citation:

PDF (3373 KB)

Purification and Composition of Polysaccharides from Pseudostellaria heterophylla and Protective Effect on Raw 264.7 Cells Induced by LPS

1.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
2.
Anhui Wannan Tobacco Leaf Co., Ltd., Xuancheng 242000, China

More Information

Received Date: October 27, 2021
Available Online: May 09, 2022

Graphical Abstract

Abstract

Abstract

To determine the composition and protective effect on Raw 264.7 cells of polysaccharide from Pseudostellaria heterophylla as a traditional Chinese medicinal, the main active ingredient-Pseudostellaria heterophylla polysaccharide (PHP) was extracted by water, precipitated by ethanol, and purified by anion exchange column chromatography. Gel permeation chromatography (GPC) and high performance liquid chromatography (HPLC) were used to determine molecular weight and monosaccharide composition of PHP. Ultraviolet spectroscopy (UV) and Fourier transform infrared spectroscopy (FT-IR) were used for primary structural characterization of PHP. Furthermore, the Raw 264.7 cell model was used to explore protective effect of PHP on Raw 264.7 cells induced by LPS. The results showed that the yield of crude polysaccharide of Pseudostellaria heterophylla was 8.23%, and the polysaccharide content was 51.47%. After purification by DEAE-52 column chromatography, the polysaccharide content increased to 80.52%, and the molecular weight distribution was between 500 to 92×10³ Da. The monosaccharide components of PHP include glucose, galactose, arabinose, mannose and glucuronic acid. Cell experiments showed that the safe dose of PHP was 80~640 µg/mL and could increase the survival rate of cells induced by lipopolysaccharide (LPS). At a dose of 640 µg/mL, the survival rate increased by 7.21%, and it was found that PHP could improve the morphology of Raw 264.7 cells. In summary, in this study the polysaccharides from Pseudostellaria heterophylla were purified, the structure of PHP was characterized, and it was preliminarily confirmed that PHP had potential protective effect on LPS-induced damage to Raw 264.7 cells. This laid the foundation for the follow-up exploration of functional activity, structure-effect and dose-effect of PHP.
- Pseudostellaria heterophylla polysaccharide,
- monosaccharide composition,
- Raw 264.7 cells,
- protective effects

FullText(HTML)

References (46)

References

[1]	刘训红, 阚毓铭. 太子参研究概述[J]. 时珍国医国药,2000,11(12):1131−1132. [LIU Xunhong, KAN Yuming. Overview of the research on Pesudostellaria heterophylla[J]. Lishizhen Medicine and Materia Medica Research,2000,11(12):1131−1132. doi: 10.3969/j.issn.1008-0805.2000.12.078 LIU Xunhong, KAN Yuming. Overview of the research on Pesudostellaria heterophylla[J]. Lishizhen Medicine and Materia Medica Research, 2000, 11(12): 1131-1132. doi: 10.3969/j.issn.1008-0805.2000.12.078
[2]	SUN H W, SHI K Q, QI K, et al. Pseudostellaria heterophylla extract polysaccharide H-1-2 suppresses pancreatic cancer by inhibiting hypoxia-induced AG2[J]. Molecular Therapy-Oncolytics,2020,17:61−69. doi: 10.1016/j.omto.2020.03.007
[3]	YOU S Y, LIU X W, XU G T, et al. Identification of bioactive polysaccharide from Pseudostellaria heterophylla with its anti-inflammatory effects[J]. Journal of Functional Foods,2021,78(150):104353.
[4]	陈耀金, 陈建洪, 刘伟招, 等. 太子参胶囊增强免疫作用实验研究[J]. 中医药临床杂志,2015,27(8):1172−1174. [CHEN Yaojin, WANG Gong, CHEN Jianhong, et al. Study on Taizishen capsule’s functions in enhancing immunity[J]. Clinical Journal of Traditional Chinese Medicine,2015,27(8):1172−1174. CHEN Yaojin, WANG Gong, CHEN Jianhong, et al. Study on Taizishen capsule’s functions in enhancing immunity[J]. Clinical Journal of Traditional Chinese Medicine, 2015, 27(8): 1172-1174.
[5]	张炎达, 潘慧青, 衣伟萌, 等. 太子参须提取物对小鼠免疫功能的影响[J]. 现代畜牧兽医,2021(10):46−49. [ZHANG Yanda, PAN Huiqing, YI Weimeng, et al. Effect of Pseudostellaria heterophylla fibrous root extraction on immunologic functions of mice[J]. Graziery Veterinary Sciences,2021(10):46−49. ZHANG Yanda, PAN Huiqing, YI Weimeng, et al. Effect of Pseudostellaria heterophylla fibrous root extraction on immunologic functions of mice[J]. Graziery Veterinary Sciences, 2021(10): 46-49
[6]	CHEN J L, PANG W S, KAN Y J, et al. Structure of a pectic polysaccharide from Pseudostellaria heterophylla and stimulating insulin secretion of INS-1 cell and distributing in rats by oral[J]. International Journal of Biological Macromolecules,2018,106:456−463. doi: 10.1016/j.ijbiomac.2017.08.034
[7]	FANG Z H, DUAN X C, ZHAO J D, et al. Novel polysaccharide H-1-2 from Pseudostellaria heterophylla alleviates type 2 diabetes mellitus[J]. Cellular Physiology and Biochemistry,2018,49(3):996−1006.
[8]	YANG C J, YOU L T, YIN X B, et al. Heterophyllin B ameliorates lipopolysaccharide-induced inflammation and oxidative stress in Raw264.7 macrophages by suppressing the PI3K/Akt pathways[J]. Molecules,2018,23(4):717. doi: 10.3390/molecules23040717
[9]	WANG Z, LIAO S G, HE Y, et al. Protective effects of fractions from Pseudostellaria heterophylla against cobalt chloride-induced hypoxic injury in H9c2 cell[J]. Journal of Ethnopharmacology,2013,147(2):540−545. doi: 10.1016/j.jep.2013.03.053
[10]	陈小英, 曾晏萍, 刘汉儒, 等. 太子参须多糖粗提物对小鼠免疫功能的调节作用[J]. 西南大学学报(自然科学版),2020,42(4):56−64. [CHEN Xiaoying, ZENG Yanping, LIU Hanru, et al. Effects of crude polysaccharides from Radix pseudostellariae on immunoregulation function in mice[J]. Journal of Southwest University (Natural Science Edition),2020,42(4):56−64. CHEN Xiaoying, ZENG Yanping, LIU Hanru, et al. Regulating effect of crude extract of Polysaccharides from Radix Pseudoginsengensis on the immune function of mice[J]. Journal of Southwest University (Natural Science Edition), 2020, 42(4): 56-64.
[11]	冯丹, 郝思钰, 付杨, 等. 太子参多糖注射液对免疫抑制小鼠免疫功能的影响[J]. 中兽医医药杂志,2020,39(3):74−77. [FENG Dan, HAO Siyu, FU Yang, et al. Effect of Taizishen polysaccharide injection on the immune function of immunosuppressive mice[J]. Journal of Traditional Chinese Veterinary Medicine,2020,39(3):74−77. FENG Dan, HAO Siyu, FU Yang, et al. Effect of Taizishen polysaccharide injection on the immune function of immunosuppressive mice[J]. Journal of Traditional Chinese Veterinary Medicine, 2020, 39(3): 74-77.
[12]	SARAH J, CLEMENTS, SIMON R CARDING. Diet, the intestinal microbiota, and immune health in aging[J]. Critical Reviews in Food Science and Nutrition,2018,58(4):651−661. doi: 10.1080/10408398.2016.1211086
[13]	崔石阳, 姜帆, 韩建春, 等. 北五味子多糖对RAW 264.7巨噬细胞的免疫调节作用[J]. 食品科学,2017,38(19):201−205. [CUI Shiyang, JIANG Fan, HAN Jianchun, et al. The immunomodulatory effect of Schisandra chinensis polysaccharide on Raw 264.7 cells[J]. Food Science,2017,38(19):201−205. doi: 10.7506/spkx1002-6630-201719032 CUI Shiyang, JIANG Fan, HAN Jianchun, et al. The immunomodulatory effect of Schisandra chinensis polysaccharide on Raw 264.7 cells[J]. Food Science, 2017, 38 (19): 201-205. doi: 10.7506/spkx1002-6630-201719032
[14]	HU J, PANG W S, CHEN J L, et al. Hypoglycemic effect of polysaccharides with different molecular weight of Pseudostellaria heterophylla[J]. BMC Complementary and Alternative Medicine,2013,13(1):1−9. doi: 10.1186/1472-6882-13-1
[15]	野津, 张文森, 王知斌, 等. DEAE-52在中药多糖分离纯化中的应用[J]. 化学工程师,2019,33(11):43−45,22. [YE Jin, ZHANG Wensen, WANG Zhibin, et al. Application of DEAE-52 in the separation and purification of polysaccharides from traditional Chinese medicine[J]. Chemical Engineers,2019,33(11):43−45,22. YE Jin, ZHANG Wensen, WANG Zhibin, et al. Application of DEAE-52 in the separation and purification of polysaccharides from traditional Chinese medicine[J]. Chemical Engineers, 2019, 33(11): 43-45, 22.
[16]	雷思敏, 肖榕, 章莹, 等. 铁皮石斛中性多糖分离纯化及其体内免疫调节作用研究[J]. 中药新药与临床药理,2018,29(6):748−753. [LEI Simin, XIAO Rong, ZHANG Ying, et al. Isolation and purification of neutral polysaccharides from Dendrobium candidum and its immune regulation in vivo[J]. New Chinese Medicines and Clinical Pharmacology,2018,29(6):748−753. LEI Simin, XIAO Rong, ZHANG Ying, et al. Isolation and purification of neutral polysaccharides from Dendrobium candidum and its immune regulation in vivo[J]. New Chinese Medicines and Clinical Pharmacology, 2018 , 29(6): 748-753.
[17]	陈杨扬, 李娇, 王向红, 等. 滑子菇多糖的结构及体外生物活性探究[J]. 中国食品学报,2019,19(6):68−73. [CHEN Yangyang, LI Jiao, WANG Xianghong, et al. Structural characterization and bioactivities of polysaccharides from Pholiota nameko in vitro[J]. Journal of Chinese Institute of Food Science and Technology,2019,19(6):68−73. CHEN Yangyang, LI Jiao, WANG Xianghong, et al. Structural characterization and bioactivities of polysaccharides from Pholiota nameko in vitro[J]. Journal of Chinese Institute of Food Science and Technology, 2019, 19(6): 68-73.
[18]	宁慧娟. 灵芝子实体多糖碱性电解水提取工艺优化及其性质分析[D]. 北京: 中国农业大学, 2018. NING Huijuan. Optimization of alkaline electrolyzed water extraction and characterization of polysaccharide from Ganoderma lucidum fruiting bodies[D]. Beijing: China Agricultural University, 2018.
[19]	戴军, 朱松, 汤坚, 等. PMP柱前衍生高效液相色谱法分析杜氏盐藻多糖的单糖组成[J]. 分析测试学报,2007(2):206−210. [DAI Jun, ZHU Song, TANG Jian, et al. Analysis of monosaccharide compositions in polysaccharides from D. salina by precolumn derivatization high performance liquid chromatography[J]. Journal of Instrumental Analysis,2007(2):206−210. doi: 10.3969/j.issn.1004-4957.2007.02.015 DAI Jun, ZHU Song, TANG Jian, et al. Analysis of monosaccharide compositions in polysaccharides from D. Salina by precolumn derivatization high performance liquid chromatography[J]. Journal of Instrumental Analysis, 2007(2): 206-210. doi: 10.3969/j.issn.1004-4957.2007.02.015
[20]	LEE S M, Y M, P H. Protective effects of Paeonia lactiflora pall on hydrogen peroxide-inducedapoptosis in PC12 cells[J]. Bioscience, Biotechnology and Biochemistry,2008,5(72):1272−1277.
[21]	JIN S E, KIM O S, YOO S, et al. Anti-inflammatory effect and action mechanisms of traditional herbal formula Gamisoyo-san in Raw264.7 macrophages[J]. BMC Complementary and Alternative Medicine,2016,16(1):1−11.
[22]	林巧美, 庞文生, 曾洁, 等. 福建柘荣不同品种太子参浸出物及多糖含量测定[J]. 中国民族民间医药,2019,28(3):21−23. [LIN Qiaomei, PANG Wensheng, ZENG Jie, et al. Experimental study on the extracts and polysaccharides of different sarieties of Pseudostellaria heterophylla from Zhelong Fujian province[J]. Chinese Journal of Ethnomedicine and Ethnopharmacy,2019,28(3):21−23. LIN Qiaomei, PANG Wensheng, ZENG Jie, et al. Experimental study on the extracts and polysaccharides of different sarieties of Pseudostellaria heterophylla from Zhelong Fujian province[J]. Chinese Journal of Ethnomedicine and Ethnopharmacy, 2019, 28(3): 21-23.
[23]	闵思明, 赵晓瑶, 陈赛红, 等. 太子参参须多糖对免疫抑制小鼠的免疫调节作用研究[J]. 动物医学进展,2020,41(8):23−28. [MIN Siming, ZHAO Xiaoyao, CHEN Saihong, et al. Immunomodulatory effects of radix pseudostellariae fibrous root polysaccharides on cyclophosphamide-induced immunosuppressed mice[J]. Progress in Veterinary Medicine,2020,41(8):23−28. doi: 10.3969/j.issn.1007-5038.2020.08.005 MIN Siming, ZHAO Xiaoyao, CHEN Saihong, et al. Immunomodulatory effects of radix Pseudostellariae fibrous root polysaccharides on cyclophosphamide-induced immunosuppressed mice[J]. Progress in Veterinary Medicine, 2020, 41(8): 23-28. doi: 10.3969/j.issn.1007-5038.2020.08.005
[24]	陶俊, 文汉. 油茶籽多糖分离纯化和结构分析[J]. 食品工业科技,2011,32(6):132−135. [TAO Jun, WEN Han. Isolation, purification and structural analysis of Camellia seed polysaccharides[J]. Science and Technology of Food Industry,2011,32(6):132−135. TAO Jun, WEN Han. Isolation, Purification and structural analysis of Camellia seed polysaccharides[J]. Science and Technology of Food Industry, 2011, 32(6): 132-135.
[25]	张发宇, 余金卫, 张浏, 等. 巢湖蓝藻藻蓝蛋白纯化过程中紫外-可见吸收光谱特征分析[J]. 光谱学与光谱分析,2017,37(3):806−810. [ZHANG Fayu, YU Jinwei, ZHANG Liu, et al. Characteristic analysis of ultraviolet-visible absorption spectrum during purification of cyanobacterial cyanophytein in Chaohu Lake[J]. Spectroscopy and Spectral Analysis,2017,37(3):806−810. ZHANG Fayu, YU Jinwei, ZHANG Liu, et al. Characteristic analysis of ultraviolet-visible absorption spectrum during purification of cyanobacterial cyanophytein in Chaohu Lake[J]. Spectroscopy and Spectral Analysis, 2017, 37(3): 806-810.
[26]	董洲. 野葛根多糖的提取、分离纯化、结构鉴定及对小鼠巨噬细胞RAW264.7的免疫调节活性研究[D]. 广州: 华南理工大学, 2018. DONG Zhou. Extraction, isolation, purification, structural characterization and immunomodulatory activity in RAW264.7 cells of the polysaccharides from Pueraria lobate (willd. ) Ohwi root[D]. Guangzhou: South China University of Technology, 2018.
[27]	黄娜. 血清色素上皮衍生因子(PEDF)与糖尿病肾病蛋白尿的相关性及其机制研究[D]. 上海: 中国人民解放军陆军军医大学, 2019. HUANG Na. The effect and mechanism of increased levels of serum pigment epithelium-derived factor in proteinuria in diabetic kidney disease[D]. Shanghai: Army Medical University, 2019.
[28]	HAN L, SUO Y, YANG Y, et al. Optimization, characterization, and biological activity of polysaccharides from Berberis dasystachya Maxim[J]. International Journal of Biological Macromolecules,2016,85:655−666. doi: 10.1016/j.ijbiomac.2015.10.038
[29]	ZHANG L, ZHANG W, WANG Q, et al. Purification, antioxidant and immunological activities of polysaccharides from Actinidia chinensis roots[J]. International Journal of Biological Macromolecules,2015,72:975−983. doi: 10.1016/j.ijbiomac.2014.09.056
[30]	LÜ X, CHEN D, YANG L, et al. Comparative studies on the immunoregulatory effects of three polysaccharides using high content imaging system[J]. International Journal of Biological Macromolecules,2016,86:28−42. doi: 10.1016/j.ijbiomac.2016.01.048
[31]	王银平. 玉木耳多糖的制备、结构表征及其与乳清蛋白相互作用研究[D]. 长春: 吉林大学, 2020. WANG Yinping. Preparation and structural characterization of polysaccharides from Auricularia cornea var. Li. and their interactions with whey protein[D]. Changchun: Jilin University, 2020.
[32]	史文涛, 庞文生, 胡娟. 高效凝胶色谱法测定太子参均一多糖分子量[J]. 中国民族民间医药,2015,24(2):20−30. [SHI Wentao, PANG Wensheng, HU Juan. Determination of the molecular weight of uniform polysaccharides of radix pseudostellariae by high performance gel chromatography[J]. Chinese National Folk Medicine,2015,24(2):20−30. SHI Wentao, PANG Wensheng, HU Juan. Determination of the molecular weight of uniform polysaccharides of Radix Pseudostellariae by high performance gel chromatography[J]. Chinese National Folk Medicine, 2015, 24(2): 20-30.
[33]	杨斌, 庞文生, 胡娟. 太子参均一多糖的分离与表征[J]. 中国民族民间医药,2017,26(4):11−16. [YANG Bin, PANG Wensheng, HU Juan. Isolation and characterization of homogeneous polysaccharides from radix pseudostellariae[J]. Chinese National Folk Medicine,2017,26(4):11−16. YANG Bin, PANG Wensheng, HU Juan. Isolation and characterization of homogeneous polysaccharides from Radix Pseudostellariae[J]. Chinese National Folk Medicine, 2017, 26(4): 11-16.
[34]	刘艳红, 唐祥, 李娅琳, 等. 活性多糖提取纯化及结构解析的研究进展[J]. 中国民族民间医药,2020,29(3):67−73. [LIU Yanhong, TANG Xiang, LI Yalin, et al. Research progress on the extraction and purification and structure analysis of active polysaccharides[J]. Chinese Ethnic Folk Medicine,2020,29(3):67−73. LIU Yanhong, TANG Xiang, LI Yalin, et al. Research progress on the extraction and purification and structure analysis of active polysaccharides[J]. Chinese Ethnic Folk Medicine, 2020, 29(3): 67-73.
[35]	李红法, 郭松波, 满淑丽, 等. 乙醇分级沉淀提取黄芪多糖及其理化性质和抗氧化活性研究[J]. 中国中药杂志,2015,40(11):2111−2116. [LI Hongfa, GUO Songbo, MAN Shuli, et al. Extraction of Astragalus polysaccharide by ethanol fractional precipitation and its physical and chemical properties and antioxidant activity[J]. China Journal of Chinese Materia Medica,2015,40(11):2111−2116. LI Hongfa, GUO Songbo, MAN Shuli, et al. Extraction of Astragalus polysaccharide by ethanol fractional precipitation and its physical and chemical properties and antioxidant activity[J]. China Journal of Chinese Materia Medica, 2015, 40(11): 2111-2116.
[36]	郭守斌. 柱前衍生超高效液相色谱法分析太子参多糖中单糖的组成[J]. 中国现代医学杂志,2016,26(23):37−41. [GUO Shoubin. Analysis of monosaccharide composition of Pseudostellaria heterophylla polysaccharides by pre-column derivatization ultra performance liquid chromatography[J]. China Journal of Modern Medicine,2016,26(23):37−41. doi: 10.3969/j.issn.1005-8982.2016.23.008 GUO Shoubin. Analysis of monosaccharide composition of Pseudostellaria heterophylla polysaccharides by pre-column derivatization ultra performance liquid chromatography[J]. China Journal of Modern Medicine, 2016, 26(23): 37-41. doi: 10.3969/j.issn.1005-8982.2016.23.008
[37]	夏和先, 陈乃东, 姚厚军, 等. 不同种源的太子参多糖含量及其单糖组成GC-MS研究[J]. 天然产物研究与开发,2016,28(4):542−546. [XIA Hexian, CHEN Naidong, YAO Houjun, et al. Study on the polysaccharide content and monosaccharide composition of Pseudostellaria heterophylla from different provenances by GC-MS[J]. Natural Products Research and Development,2016,28(4):542−546. XIA Hexian, CHEN Naidong, YAO Houjun, et al. Study on the polysaccharide content and monosaccharide composition of Pseudostellaria heterophylla from different provenances by GC-MS[J]. Natural Products Research and Development, 2016, 28(4): 542-546.
[38]	商佳琦, 邹丹阳, 滕翔宇, 等. 5种食用菌多糖的结构特征及抗氧化活性对比[J]. 食品工业科技,2020,41(15):77−83. [SHANG Jiaqi, ZOU Danyang, TENG Xiangyu, et al. Structural characterization and antioxidant activity of five kinds of edible fungus polysaccharides[J]. Science and Technology of Food Industry,2020,41(15):77−83. SHANG Jiaqi, ZOU Danyang, TENG Xiangyu, et al. Structural characterization and antioxidant activity of five kinds of edible fungus polysaccharides[J]. Science and Technology of Food Industry, 2020, 41(15): 77-83.
[39]	宋立立, 张兆英. 食用菌多糖的生物活性研究现状[J]. 中国食用菌,2019,38(2):14−16. [SONG Lili, ZHANG Zhaoying. The status of biological activity of mushroom polysaccharides[J]. Edible Fungi of China,2019,38(2):14−16. SONG Lili, ZHANG Zhaoying. The status of biological activity of mushroom polysaccharides[J]. Edible Fungi of China, 2019, 38(2): 14-16.
[40]	张丽娟, 王珍, 廖尚高. 太子参多糖对RAW 264.7巨噬细胞免疫调节作用的初步研究[J]. 中国野生植物资源,2018,37(4):14−17. [ZHANG Lijuan, WANG Zhen, LIAO Shanggao. Preliminary study on the immunomodulatory activity of Pseudostellaria polysaccharides on macrophage cell line RAW 264.7[J]. Chinese Wild Plant Resources,2018,37(4):14−17. doi: 10.3969/j.issn.1006-9690.2018.04.003 ZHANG Lijuan, WANG Zhen, LIAO Shanggao. Preliminary study on the immunomodulatory activity of Pseudostellaria polysaccharides on macrophage cell line RAW 264.7[J]. Chinese Wild Plant Resources, 2018, 37(4): 14-17. doi: 10.3969/j.issn.1006-9690.2018.04.003
[41]	方瑶, 毛旭虎. 小鼠巨噬细胞RAW264.7的培养技巧及经验总结[J]. 现代生物医学进展,2012,12(22):4358−4359. [FANG Yao, MAO Xuhu. Cultivation skills and experience summary of mouse macrophage RAW264.7[J]. Progress in Modern Biomedicine,2012,12(22):4358−4359. FANG Yao, MAO Xuhu. Cultivation skills and experience summary of mouse macrophage RAW264.7[J]. Progress in Modern Biomedicine, 2012, 12(22): 4358-4359.
[42]	张勇. 铁皮石斛茎、叶、花多糖理化性质及抗氧化、免疫调节活性研究[D]. 杭州: 浙江大学, 2016. ZHANG Yong. Study on the physicochemical properties and antioxidant and immunomodulatory activities of polysaccharides from stems, leaves and flowers of Dendrobium candidum[D]. Hangzhou: Zhejiang University, 2016.
[43]	亢玉莹. 七种真菌多糖免疫活性的筛选及杏鲍菇多糖免疫机制的研究[D]. 长春: 东北师范大学, 2018. KANG Yuying. Screening of seven fungal polysaccharides for immune activity and study on immune mechanism of Pleurotus eryngii polysaccharide[D]. Changchun: Northeast Normal University, 2018.
[44]	王成蓉, 吴金华, 涂丽蓉, 等. 肉苁蓉多糖对鸡免疫ND苗后血液中T淋巴细胞及IFN-γ活性的影响[J]. 四川畜牧兽医,2008(8):29−30,32. [WANG Chengrong, WU Jinhua, TU Lirong, et al. Effects of Cistanche deserticola polysaccharides on T lymphocytes and IFN-γ activity in the blood of chickens immunized with ND vaccine[J]. Sichuan Animal and Veterinary Sciences,2008(8):29−30,32. doi: 10.3969/j.issn.1001-8964.2008.08.013 WANG Chengrong, WU Jinhua, TU Lirong, et al. Effects of Cistanche deserticola polysaccharides on T lymphocytes and IFN-γ activity in the blood of chickens immunized with ND vaccine[J]. Sichuan Animal and Veterinary Sciences, 2008(8): 29-30, 32. doi: 10.3969/j.issn.1001-8964.2008.08.013
[45]	郑胜眉, 周兴, 黄文涛, 等. 岩白菜素对LPS诱导RAW264.7巨噬细胞炎性因子产生及细胞形态变化的影响[J]. 中药材,2020,43(1):206−210. [ZHENG Shengmei, ZHOU Xing, HUANG Wentao, et al. Effects of Bergenin on LPS-induced inflammatory factor production and cell morphological changes in Raw264.7 cells[J]. Chinese Medicinal Materials,2020,43(1):206−210. ZHENG Shengmei, ZHOU Xing, HUANG Wentao, et al. Effects of Bergenin on LPS-induced inflammatory factor production and cell morphological changes in Raw264.7 cells[J]. Chinese Medicinal Materials, 2020, 43(1): 206-210.
[46]	陈广勇, 韩乾杰, 张玲玲, 等. 人参多糖对脂多糖刺激小鼠巨噬细胞的免疫调控作用[J]. 动物健康,2021,57(2):182−186. [CHEN Guangyong, HAN Ganjie, ZHANG Lingling, et al. The immune regulation effect of ginseng polysaccharide on lipopolysaccharide-stimulated mouse macrophages[J]. Animal Health,2021,57(2):182−186. CHEN Guangyong, HAN Ganjie, ZHANG Lingling, et al. The immune regulation effect of ginseng polysaccharide on lipopolysaccharide-stimulated mouse macrophages[J]. Animal Health, 2021, 57(2): 182-186.

Supplements (0)

Cited By

Cited by

Periodical cited type(6)

1.	郭利健. 茶多酚与表面活性剂的相互作用及其在食品工业中的应用. 安徽化工. 2025(01): 26-29 .
2.	郝吉明. 基于淀粉作用的大米品质提升策略研究. 粮油与饲料科技. 2024(08): 225-227 .
3.	郭硕，刘景圣，郑明珠. 热处理过程中食品组分与淀粉相互作用研究进展. 食品安全质量检测学报. 2023(01): 17-24 .
4.	华玮，熊丽娜，张永鑫，孙鹏，岳龙，付鸿博. 草莓多酚提取工艺优化及抗氧化性分析. 果树资源学报. 2023(06): 17-22 .
5.	李舒玥，吴昊怡，易阳，孙莹，彭凯迪，江雪玉. 多酚强化莲藕营养粉的制备与食用品质. 武汉轻工大学学报. 2023(05): 1-10 .
6.	柳丝菁，孙红男，马梦梅，木泰华. 淀粉-多酚复合物制备及其消化特性研究进展. 食品安全质量检测学报. 2023(22): 152-161 .