Establishment of Highly Sensitive Determination Method for Mineral Oil Contaminants in Vegetable Oil-based Canned Fish Products

YANG Mengqi; LIU Lingling; LI Bingning; WU Yanwen

doi:10.13386/j.issn1002-0306.2021100291

Volume 43 Issue 12

Jun. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(12): 317-324. > DOI: 10.13386/j.issn1002-0306.2021100291

YANG Mengqi, LIU Lingling, LI Bingning, et al. Establishment of Highly Sensitive Determination Method for Mineral Oil Contaminants in Vegetable Oil-based Canned Fish Products[J]. Science and Technology of Food Industry, 2022, 43(12): 317−324. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100291.

Citation:

PDF (2763 KB)

Establishment of Highly Sensitive Determination Method for Mineral Oil Contaminants in Vegetable Oil-based Canned Fish Products

Institute of Analysis and Testing, Beijing Academy of Science and Technology (Beijing Center for Physical and Chemical Analysis), Beijing Food Safety Analysis and Testing Engineering Research Center, Beijing 100094, China

More Information

Received Date: October 27, 2021
Available Online: April 14, 2022

Graphical Abstract

Abstract

Abstract

A method for the high-sensitive determination of mineral oil hydrocarbons (MOH) in vegetable oil-based canned fish was established, and applied in some commercial products. The samples were extracted and purified by saponification and epoxidation, and were fractionated, concentrated and quantitatively determined by on-line high performance liquid chromatography coupled with gas chromatography (HPLC-GC) for mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH). The results showed that the limit of quantification (LOQ) of the method was 0.5 mg/kg and the spiked recovery was 90.0%~106.0%, and the precision (RSD) was 1.6%~12.7%, which met the requirements of the European Joint Research Center (JRC) for the analysis of MOH in the higher-fat/oil-content foods. This method was applied to detect ten canned fish products sold in Beijing. It was found that all of those contained MOSH with contents of 2.6~53.7 mg/kg, of which five detected MOAH with a content of 0.7~5.5 mg/kg. The MOH chromatographic profiles indicated that the contaminants were closely related to the sources of the vegetable oils and fish meat in those products.

FullText(HTML)

References (30)

References

[1]	European Food Safety Authority (EFSA). Scientific opinion on mineral oil hydrocarbons in food[J]. EFSA Journal,2012,10(6):2704.
[2]	GROB K. Mineral oil hydrocarbons in food–a review[J]. Food Additives & Contaminants:Part A,2018,35:9,1845−1860.
[3]	黄华, 武彦文, 李冰宁, 等. 食用植物油中矿物油污染物的分析进展[J]. 中国油脂,2018,43(7):97−101. [HUANG H, WU Y W, LI B N, et al. Progress in analysis of mineral oil contaminants in edible vegetable oils[J]. China Oils and Fats,2018,43(7):97−101. doi: 10.3969/j.issn.1003-7969.2018.07.022 HUANG H, WU Y W, LI B N, et al. Progress in analysis of mineral oil contaminants in edible vegetable oils[J]. China Oils and Fats. 2018, 43(7): 97-101. doi: 10.3969/j.issn.1003-7969.2018.07.022
[4]	BRÜHL L. Occurrence, determination, and assessment of mineral oils in oil seeds and vegetable oils[J]. European Journal of Lipid Science and Technology,2016,118(3):361−372. doi: 10.1002/ejlt.201400528
[5]	LIU L L, HUANG H, WU Y W, et al. Offline solid-phase extraction large volume injection gas chromatography for the analysis of mineral oil saturated hydrocarbons in commercial vegetable oils[J]. Journal of Oleo Science,2017,66(9):981−990. doi: 10.5650/jos.ess17081
[6]	GÓMEZ-COCA R B, CERT R, PÉREZ-CAMINO M C, et al. Determination of saturated aliphatic hydrocarbons in vegetable oils[J]. Grasas Aceites,2016,67(2):e127. doi: 10.3989/gya.0627152
[7]	李冰宁, 刘玲玲, 武彦文. 固相萃取-程序升温-气相色谱法与大容量固相萃取-气相色谱法测定食用油脂中饱和烃矿物油的比较[J]. 分析实验室,2018,37(6):701−705. [LI B N, LIU L L, WU Y W. Comparison of solid phase extraction-programmed temperature vaporizer-gas-chromatography and large scale-solid phase extraction-gas chromatography for the determination of mineral oil saturated hydrocarbons in edible oils and fats[J]. Chinese Journal of Analysis Laboratory,2018,37(6):701−705. LI B N, LIU L L, WU Y W. Comparison of solid phase extraction-programmed temperature vaporizer-gas-chromatography and large scale-solid phase extraction-gas chromatography for the determination of mineral oil saturated hydrocarbons in edible oils and fats[J]. Chinese Journal of Analysis Laboratory, 2018, 37(6): 701-705.
[8]	ISO technical committees. ISO 17780-2015 Animal and vegetable fats and oils-determination of aliphatic hydrocarbons in vegetable oils[S]. Switzerland: ISO copyright office, 2015.
[9]	European Committee for Standardization (CEN) European standard (EN) 16995-2017 Foodstuffs-Vegetable oils and food-stuff on basis of vegetable oils-Determination of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) with on-line HPLC-GC-FID analysis[S]. Brussels: BSI standards Limited, 2017.
[10]	Food Federation Germany, Consumer Protection Consortium of the Federal States. Benchmark levels for mineral oil hydrocarbons (MOH) in foods [EB/OL]. [2019-05-23].https://www.lebensmittelverband.de/de/aktuell/20190502-veroeffentlichung-moh-orientierungswerte.
[11]	中华人民共和国国家质量监督检验检疫总局. GB 17378.4-2007 海洋监测规范第4部分: 海水分析[S]. 北京: 中国标准出版社, 2007 General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China. GB 17378.4-2007 The specification for marine monitoring—part 4: Seawater analysis[S]. Beijing: China Standard Press, 2007.
[12]	中华人民共和国国家质量监督检验检疫总局. GB 17378.6-2007 海洋监测规范第6部分: 生物体分析[S]. 北京: 中国标准出版社, 2007 General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China. GB 17378.6-2007 The specification for marine monitoring—part 6: Organism analysis[S]. Beijing: China Standard Press, 2007.
[13]	生态环境部. HJ 637-2018 水质石油类和动植物油类的测定红外分光光度法[S]. 北京: 中国标准出版社, 2018 Ministry of Ecology and Environment. HJ 637-2018 Water quality-determination of petroleum, animal fats and vegetable oils-infrared spectrophotometry[S]. Beijing: China Standard Press, 2018.
[14]	环境保护部. HJ 894-2017 水质可萃取性石油烃(C₁₀-C₄₀)的测定气相色谱法[S]. 北京: 中国标准出版社, 2017 Ministry of Environmental Protection. HJ 894-2017 Water quality-determination of extractable petroleum hydrocarbons(C₁₀-C₄₀)-gas chromatography[S]. Beijing: China Standard Press, 2017.
[15]	LIU L L, LI B N, YANG D Y, et al. Survey of mineral oil hydrocarbons in Chinese commercial complementary foods for infants and young children[J]. Food Additives & Contaminants: Part A,2021,38(9):1441−1455.
[16]	SRBINOVSKA A, CONCHIONE C, LUCCI P, et al. On-line HP(LC)-GC-FID determination of hydrocarbon contaminants in fresh and packaged fish and fish products[J]. Journal of AOAC International,2021,104(2):267−273. doi: 10.1093/jaoacint/qsaa144
[17]	MONDELLO L, ZOCCALI M, PURCARO G, et al. Determination of saturated-hydrocarbon contamination in baby foods by using on-line liquid–gas chromatography and off-line liquid chromatography-comprehensive gas chromatography combined with mass spectrometry[J]. Journal of Chromatography A,2012,1259:221−226. doi: 10.1016/j.chroma.2012.03.096
[18]	BIEDERMANN M, GROB K. On-line coupled high performance liquid chromatography–gas chromatography for the analysis of contamination by mineral oil. Part 1: Method of analysis[J]. Journal of Chromatography A,2012,1255:56−75. doi: 10.1016/j.chroma.2012.05.095
[19]	武彦文, 李冰宁, 刘玲玲, 等. 基于高效液相色谱-气相色谱联用技术的矿物油分析研究进展[J]. 分析化学,2021,49(3):341−349. [WU Y W, LI B N, LIU L L, et al. Research progress of mineral oil analysis based on high performance liquid chromatography-gas chromatography[J]. Chinese Journal of Analytical Chemistry,2021,49(3):341−349. doi: 10.1016/S1872-2040(21)60084-1 WU Y W, LI B N, LIU L L, et al. Research progress of mineral oil analysis based on high performance liquid chromatography-gas chromatography[J]. Chinese Journal of Analytical Chemistry, 2021, 49(03): 341-349. doi: 10.1016/S1872-2040(21)60084-1
[20]	ARES. 2019. Summary of the roundtable workshop on the determination of MOAH in infant formula[C]. European Commission Joint Research Centre. 7564336-09/12/2019.
[21]	BRATINOVA S, HOEKSTRA E. Guidance on sampling, analysis and data reporting for the monitoring of mineral oil hydrocarbons in food and food contact materials[Z]. Luxembourg: Publications Office of the European Union, 2019, ISBN 978-92-76-00172-0,doi:10.2760/208879, JRC115694.
[22]	BIEDERMANN M, FISELIER K, GROB K. Aromatic hydrocarbons of mineral oil origin in foods: Method for determining the total concentration and first results[J]. Journal of Agricultural and Food Chemistry,2009,57(19):8711−8721. doi: 10.1021/jf901375e
[23]	NESTOLA M, SCHMIDT T C. Determination of mineral oil aromatic hydrocarbons in edible oils and fats by online liquid chromatography-gas chromatography-flame ionization detection-evaluation of automated removal strategies for biogenic olefins[J]. Journal of Chromatography A,2017,1505:69−76. doi: 10.1016/j.chroma.2017.05.035
[24]	BIEDERMANN M, MUNOZ C, GROB K. Epoxidation for the analysis of the mineral oil aromatic hydrocarbons in food. an update[J]. Journal of Chromatography A,2020,1624:461236. doi: 10.1016/j.chroma.2020.461236
[25]	刘玲玲, 武彦文, 李冰宁, 等. 固相萃取-大体积进样-气相色谱法定量分析油茶籽油中的矿物油[J]. 分析化学,2016,44(9):1419−1424. [LIU L L, WU Y W, LI B N, et al. Quantitative analysis of mineral oil in Camellia oleifera seed oil by solid phase extraction-mass injection-gas chromatography[J]. Chinese Journal of Analytical Chemistry,2016,44(9):1419−1424. doi: 10.11895/j.issn.0253-3820.160138 LIU L L, WU Y W, LI B N, et al. Quantitative analysis of mineral oil in Camellia oleifera seed oil by solid phase extraction-mass injection-gas chromatography[J]. Chinese Journal of Analytical Chemistry, 2016, 44(9): 1419-1424. doi: 10.11895/j.issn.0253-3820.160138
[26]	刘玲玲, 李冰宁, 欧阳杰, 等. 高效液相色谱-气相色谱联用法测定稻谷和大米中的饱和烃矿物油[J]. 食品科学,2019,40(16):302−307. [LIU L L, LI B N, OUYANG J, et al. Determination of mineral oil-saturated hydrocarbons in paddy and rice by on-line high performance liquid chromatography-gas chromatography[J]. Food Science,2019,40(16):302−307. doi: 10.7506/spkx1002-6630-20180627-498 LIU L L, LI B N, OUYANG J, et al. Determination of mineral oil-saturated hydrocarbons in paddy and rice by on-line high performance liquid chromatography-gas chromatography[J]. Food Science, 2019, 40(16): 302-307. doi: 10.7506/spkx1002-6630-20180627-498
[27]	BARP L, BIEDERMANN M, GROB K, et al. Accumulation of mineral oil saturated hydrocarbons (MOSH) in female Fischer344 rats: Comparison with human data and consequences for risk assessment[J]. Science of the Total Environment,2017,575(9):1263−1278.
[28]	GROB K. Toxicological assessment of mineral hydrocarbons in foods: State of present discussions[J]. Journal of Agricultural and Food Chemistry,2018,66:6968−6974. doi: 10.1021/acs.jafc.8b02225
[29]	RALPH P, ANNEGRET B, NICOLE H, et al. Mineral oil in food, cosmetic products, and in products regulated by other legislations[J]. Critical Reviews in Toxicology,2019,49(9):742−789. doi: 10.1080/10408444.2019.1694862
[30]	LI B N, WU Y W, LIU L L, et al. Determination of mineral oil-saturated hydrocarbons (MOSH) in vegetable oils by large scale off-line SPE combined with GC-FID[J]. Journal of American Oil Chemists' Society,2017,94(2):215−223. doi: 10.1007/s11746-016-2936-0

Supplements (0)

Cited By

Cited by

Periodical cited type(4)

1.	任娟，李冰宁，刘玲玲，陈婷，刘清珺，武彦文. 基于多重二阶差商滤波的矿物油检测自动判别方法. 分析化学. 2025(01): 104-114 .
2.	杨梦奇，刘鑫宇，李冰宁，欧阳雨琦，刘玲玲，李婷，武彦文. 速食食品纸桶包装冲泡过程的矿物油迁移试验研究. 食品安全质量检测学报. 2024(12): 174-181 .
3.	罗振玲，高海波，杨挺，付余. 超高效液相色谱-串联质谱法同时测定小黄花鱼中9种生物胺. 食品工业科技. 2023(05): 251-257 . 本站查看
4.	刘玲玲，李冰宁，杨梦奇，李婷，武彦文. 奶粉中饱和烃与芳香烃矿物油的高灵敏检测方法建立. 食品工业科技. 2023(20): 298-304 . 本站查看