Sedative, Hypnotic and Anti-anxiety Effects of Lignans from <i>Schisandra chinensis</i> Vine Stem in Mice

CHEN Hongxu; YUAN Liwei; ZHANG Junxiong; WANG Wenxin; LIU Jiale; WANG Chunmei; SUN Jinghui; ZHANG Chengyi; LI He; CHEN Jianguang

doi:10.13386/j.issn1002-0306.2021100209

Volume 43 Issue 14

Jul. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(14): 385-391. > DOI: 10.13386/j.issn1002-0306.2021100209

CHEN Hongxu, YUAN Liwei, ZHANG Junxiong, et al. Sedative, Hypnotic and Anti-anxiety Effects of Lignans from Schisandra chinensis Vine Stem in Mice[J]. Science and Technology of Food Industry, 2022, 43(14): 385−391. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100209.

Citation:

PDF (2463 KB)

Sedative, Hypnotic and Anti-anxiety Effects of Lignans from Schisandra chinensis Vine Stem in Mice

1.
College of Pharmacy, Beihua University, Jilin 132000, China
2.
Jilin Central Hospital, Jilin 132000, China

More Information

Received Date: October 20, 2021
Available Online: May 08, 2022

Graphical Abstract

Abstract

Abstract

Objective: To study thesedative, anti-anxiety and hypnotic effects of Schisandra chinensis vine stem lignans (SSL) and its underlying mechanism. Methods: One hundred and fifty ICR male mice were evenly and randomly divided into 5 groups, namely the blank control group, the SSL low-dose group (35 mg/kg), middle-dose group (70 mg/kg), and high-dose group (140 mg/kg), and diazepam positive control group (4 mg/kg). All treatments were administered intragastrically, 0.1 mL/10 g and once daily for 7 days. The autonomous activities of mice were used to observe the sedative effect, the elevated plus maze and elevated zero Maze were used to observe the anti-anxiety effect. The synergistic sleep test of pentobarbital sodium at subthreshold and threshold doses was used to observe the hypnotic effect. Enzyme-linked immunosorbent assay (ELISA) was used to detect the content of γ-aminobutyric acid (GABA) and glutamate (Glu) in mouse brain tissue. Results: In comparison to the blank control group, the numbers of standing and spontaneous activities of the mice was significantly reduced (P<0.01) in all SSL groups. The open arm time, the numbers of open arm entry and open arm probe significantly increased (P<0.05 or P<0.01) in SSL middle- and high-dose groups. The number of sleep mice under the subthreshold dose of pentobarbital sodium increased in all SSL groups, the sleep latency was shortened and sleep time increased in mice under the threshold dose of pentobarbital sodium in all SSL groups (P<0.05 or P<0.01). The content of GABA in brain tissue significantly increased (P<0.01) in all SSL groups, while Glu content significantly decreased (P<0.01) and GABA/Glu ratio significantly increased (P<0.01) in SSL middle- and high-dose groups, and the expression of GABA_Aα1 protein in brain tissue of mice significantly increased (P<0.01) in SSL high-dose group. Conclusion: SSL had sedative, hypnotic and anti-anxiety effects in mice, the mechanism might be related to the regulation of GABA content in the brain tissue.
- Schisandra chinensis vine stem,
- lignan,
- sedative and hypnotic,
- anti-anxiety

FullText(HTML)

References (32)

References

[1]	KORKUTATA M, SAITOH T, CHERASSE Y, et al. Enhancing endogenous adenosine A receptor signaling induces slow-wave sleep without affecting body temperature and cardiovascular function[J]. Neuropharmacology,2019,144:122−132. doi: 10.1016/j.neuropharm.2018.10.022
[2]	SHI Y, DONG J W, ZHAO J H, et al. Herbal insomnia medications that target GABAergic systems: A review of the psychopharmacological evidence[J]. Curr Neuropharmacol,2014,12:289−302. doi: 10.2174/1570159X11666131227001243
[3]	SHI M, PIAO J, XU X, et al. Chinese medicines with sedative–hypnotic effects and their active components[J]. Sleep Medicine Reviews,2016,9:108−118.
[4]	SHRGI J L, NI X, S, ARRIS J, et al. Ziziphus spinosa seeds for insomnia: A review of chemistry and psychopharmacology[J]. Phytomedicine,2017,34:38−43. doi: 10.1016/j.phymed.2017.07.004
[5]	余黄合, 李鑫, 杨珍, 等. 中药五味子药理作用研究进展[J]. 环球中医药,2019,12(07):1133−1138. [YU H H, LI X, YANG Z, et al. Research progress in pharmacological effects of Fructus Schisandra Chinensis[J]. Global Traditional Chinese Medicine,2019,12(07):1133−1138. doi: 10.3969/j.issn.1674-1749.2019.07.042 YU H H, LI X, YANG Z, et al. Research progress in pharmacological effects of Fructus Schisandra Chinensis[J]. Global Traditional Chinese Medicine, 2019, 12(07): 1133-1138. doi: 10.3969/j.issn.1674-1749.2019.07.042
[6]	牛佳牧, 刘嘉玮, 林慧娇, 等. 五味子酸性多糖对小鼠抗疲劳作用的实验研究[J]. 北华大学学报(自然科学版),2020,21(6):730−733. [NIU J M, LIU J W, LIN H J, et al. Experimental study on anti-fatigue effect of Schisandra chinensis acid polysaccharide on mice[J]. Journal of Beihua University (Natural Science),2020,21(6):730−733. NIU J M, LIU J W, LIN H J, et al. Experimental study on anti-fatigue effect of Schisandra chinensis acid polysaccharide on mice[J]. Journal of Beihua University (Natural Science), 2020, 21(6): 730-733.
[7]	YANG B Y, HAN W, HAN H, et al. Effects of Lignans from schisandra chinensis rattan stems against Aβ1-42-induced memory impairment in rats and neurotoxicity in primary neuronal cells[J]. Molecules,2018,23(4):870. doi: 10.3390/molecules23040870
[8]	李薇, 于佳卉, 王新婷, 等. 北五味子总木脂素对环磷酰胺诱导的免疫功能低下小鼠的调节作用[J]. 吉林大学学报(医学版),2019,45(2):294−299,472. [LI W, YU J H, WANG X T, et al. Immunomodulatory effect Schisandra chinensis lignans in cyclophosphamide-induced immunocompromised mice[J]. Journal of Jilin University (Medicine Edition),2019,45(2):294−299,472. LI W, YU J H, WANG X T, et al. Immunomodulatory effect Schisandra chinensis lignans in cyclophosphamide-induced immunocompromised mice[J]. Journal of Jilin University (Medicine Edition), 2019, 45(2): 294-299, 472.
[9]	ZHU H Y, ZHANG L N, WANG G L, et al. Sedative and hypnotic effects of supercritical carbon dioxide fluid extraction from Schisandra chinensis in mice[J]. Journal of Food and Drug Analysis,2016,24(4):831−838. doi: 10.1016/j.jfda.2016.05.005
[10]	陶雪, 车金营, 杨硕, 等. 五味子多糖对2型糖尿病大鼠肝脏的保护作用[J]. 北华大学学报(自然科学版),2019,20(1):43−46. [TAO X, CHE J Y, YANG S, et al. Protective Effects of Schisandra chinensis Polysaccharides on Liver in Rats with T2DM[J]. Journal of Beihua University (Natural Science),2019,20(1):43−46. TAO X, CHE J Y, YANG S, et al. Protective Effects of Schisandra chinensis Polysaccharides on Liver in Rats with T2DM[J]. Journal of Beihua University (Natural Science), 2019, 20(1): 43-46.
[11]	胡波, 孙红霞, 李贺, 等. 五味子有效成分保护心室重构的研究进展[J]. 北华大学学报(自然科学版),2018,19(1):34−38. [HU B, SUN H X, LI H, et al. Research advances of active ingredients of Schisandra chinensis on the protection of ventricular remodeling[J]. Journal of Beihua University (Natural Science),2018,19(1):34−38. HU B, SUN H X, LI H, et al. Research advances of active ingredients of Schisandra chinensis on the protection of ventricular remodeling[J]. Journal of Beihua University (Natural Science), 2018, 19(1): 34-38.
[12]	ZHON S, LIU X D, NIE Y C, et al. Antitussive activity of the Schisandra chinensis fruit polysaccharide (SCFP-1) in guinea pigs models[J]. Journal of ethnopharmacology,2016,194:378−385. doi: 10.1016/j.jep.2016.08.008
[13]	石绘, 万丽华, 李贺, 等. 北五味子木脂素对小鼠镇静催眠作用的实验研究[J]. 中国老年保健医学,2012,10(5):27−28. [SHI H, WAN L H, LI H, et al. Experimental study on sedative and hypnotic effects of Schisandra chinensis Lignans on mice[J]. Chinese Journal of Geriatric Care,2012,10(5):27−28. doi: 10.3969/j.issn.1672-4860.2012.05.011 SHI H, WAN L H, LI H, et al. Experimental study on sedative and hypnotic effects of Schisandra chinensis Lignans on mice[J]. Chinese Journal of Geriatric Care, 2012, 10(5): 27-28. doi: 10.3969/j.issn.1672-4860.2012.05.011
[14]	李贺, 李宁, 刘聪, 等. 五味子乙素对ICR小鼠的抗焦虑作用及其作用机制[J]. 吉林大学学报(医学版),2018,44(1):78−82. [LI H, LI N, LIU C, et al. Antianxiety effect of schizandrin B in ICR mice and its mechanism[J]. Journal of Jilin University (Medicine Edition),2018,44(1):78−82. LI H, LI N, LIU C, et al. Antianxiety effect of Schizandrin B in ICR mice and its mechanism[J]. Journal of Jilin University (Medicine Edition), 2018, 44(1): 78-82.
[15]	王春梅, 李贺, 孙靖辉, 等. 北五味子多糖抗焦虑和镇静催眠作用[J]. 食品科学,2015,36(13):239−242. [WANG C M, LI H, SUN J H, et al. Antianxiety, sedative and hypnotic effects of Schisandra chinensis polysaccharides[J]. Food Science,2015,36(13):239−242. doi: 10.7506/spkx1002-6630-201513044 WANG C M, LI H, SUN J H, et al. Antianxiety, sedative and hypnotic effects of Schisandra chinensis polysaccharides[J]. Food Science, 2015, 36(13): 239-242. doi: 10.7506/spkx1002-6630-201513044
[16]	中国药典委员会(编). 中华人民共和国药典[M].北京: 国家药典科学技术出版社, 2020. Chinese Pharmacopoeia Committee (edited). Pharmacopoeia of the People's Republic of China [M]. Beijing: National Pharmacopoeia Science and Technology Press, 2020.
[17]	吕辰鹏, 王丽斐, 何泉泉, 等. 改善睡眠功能保健食品的研究进展[J]. 食品工业,2017,38(8):238−243.]. [HE Q Q, et al. Research progress of health food with effect of improving sleeping[J]. Food Science,2017,38(8):238−243.]. HE Q Q, et al. Research progress of health food with effect of improving sleeping[J]. Food Science, 2017, 38(8): 238-243. ]
[18]	姜瑞平, 邵信儒, 孙海涛, 等. 北五味子藤茎调味粉的研制[J]. 中国调味品,2017,42(12):134−137. [[JIANG R P, SHAO X R, SUN H T, et al. Study on seasoning powder of caulis of Schisandra chinensis (Turcz. ) Baill[J]. China Condiment,2017,42(12):134−137. doi: 10.3969/j.issn.1000-9973.2017.12.030 [JIANG R P, SHAO X R, SUN H T, et al. Study on seasoning powder of caulis of Schisandra chinensis (Turcz. ) Baill[J]. China Condiment, 2017, 42(12): 134-137. doi: 10.3969/j.issn.1000-9973.2017.12.030
[19]	李国成, 邱凯锋, 刘恩桂, 等. 北五味子藤茎的化学成分研究[J]. 中药材,2006(10):1045−1047. [LI G C, QIU K F, LIU E G, et al. Study on the chemical constituents of the cane of Schisandra chinensis[J]. Journal of Chinese Medicinal Materials,2006(10):1045−1047. doi: 10.3321/j.issn:1001-4454.2006.10.019 LI G C, QIU K F, LIU E G, et al. Study on the chemical constituents of the cane of Schisandra chinensis[J]. Journal of Chinese Medicinal Materials, 2006(10): 1045-1047. doi: 10.3321/j.issn:1001-4454.2006.10.019
[20]	买文丽, 王琼, 刘新民, 等. 小鼠自主活动实验中的评价指标[J]. 中国实验动物学报,2008(3):172−175,244. [MAI W L, WANG Q, LIU X M, et al. Evaluation index in the experiment of autonomous activity in mice[J]. Chinese Journal of Experimental Animal,2008(3):172−175,244. doi: 10.3969/j.issn.1005-4847.2008.03.003 MAI W L, WANG Q, LIU X M, et al. Evaluation index in the experiment of autonomous activity in mice[J]. Chinese Journal of Experimental Animal, 2008(3): 172-175, 244. doi: 10.3969/j.issn.1005-4847.2008.03.003
[21]	TCHEKALAROVA J, KUBOVA H, MARES P. Postnatal caffeine exposure: effects on motor skills and locomotor activity during ontogenesis[J]. Behavioural Brain Research,2005,160(1):99−106. doi: 10.1016/j.bbr.2004.11.018
[22]	于泽鹏, 高佳琪, 刘聪, 等. 五味子醋镇静催眠抗焦虑作用及其作用机制[J]. 中国实验方剂学杂志,2018,24(11):139−143. [YU Z P, GAO J Q, LIU C, et al. Sedative, hypnotic and anxiolytic effects ang mechanism of Schisandra chinensis fructus vinegar[J]. Chinese Journal of Experimental Traditional Medical Formulae,2018,24(11):139−143. YU Z P, GAO J Q, LIU C, et al. Sedative, hypnotic and anxiolytic effects ang mechanism of Schisandra chinensis fructus vinegar[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2018, 24(11): 139-143.
[23]	PELLOW S, CHOPIN P, FILE S E, et al. Validation of open: Closed arm entries in an elevated plus-maze as a measure of anxiety in the rat[J]. Elsevier,1985,14(3):149−167.
[24]	张燕, 郑伟, 丁莹, 等. BALB/c和C57BL/6J小鼠高架十字迷宫焦虑表现行为比较[J]. 疾病预防控制通报,2012,27(3):18−20. [ZHANG Y, ZHENG W, DING Y, et al. Comparison of anxiety-like behaviors in elevated plus maze test between BALB/c and C57BL/6J mice[J]. Bulletin of Disease Control & Prevention (China),2012,27(3):18−20. ZHANG Y, ZHENG W, DING Y, et al. Comparison of anxiety-like behaviors in elevated plus maze test between BALB/c and C57BL/6J mice[J]. Bulletin of Disease Control & Prevention (China), 2012, 27(3): 18-20.
[25]	TUCKER L B, MCCABE J T. Measuring anxiety-like behaviors in rodent models of traumatic brain injury[J]. Front Behav Neurosci,2021,15:682935. doi: 10.3389/fnbeh.2021.682935
[26]	叶少剑, 黄念芳. 戊巴比妥钠多种药理效应的阈剂量观察[J]. 卫生职业教育,2014,32(24):94−95. [YE S J, HUNANG N Fg. The threshold dose observation of various pharmacological effects of pentobarbital sodium[J]. Health Vocational Education,2014,32(24):94−95. YE S J, HUNANG N Fg. The threshold dose observation of various pharmacological effects of pentobarbital sodium[J]. Health Vocational Education, 2014, 32(24): 94-95.
[27]	刘海云, 宋渺渺, 肖爱娇. 菟丝子提取物对失眠大鼠学习记忆能力及神经递质的影响[J]. 江西中医药,2018,49(9):60−62. [LIU H Y, SONG M M, XIAO A J. Effects of Cuscuta seed extract on learning and memory ability and neurotransmitters in insomnia rats[J]. Jiangxi Traditional Chinese Medicine,2018,49(9):60−62. LIU H Y, SONG M M, XIAO A J. Effects of Cuscuta seed extract on learning and memory ability and neurotransmitters in insomnia rats[J]. Jiangxi Traditional Chinese Medicine, 2018, 49(9): 60-62.
[28]	饶曼妮, 常翠, 赵奎, 等. 五味子宁神口服液对大鼠脑组织中GABA和Glu含量的影响[J]. 临床医药实践杂志,2008,1(16):739−740. [RAO M N, CHANG C, ZHAO K, et al. Effects of Wuweizi Ningshen oral liquid on GABA and Glu contents in brain tissue of rats[J]. Journal of Clinical Medicine Practice,2008,1(16):739−740. RAO M N, CHANG C, ZHAO K, et al. Effects of Wuweizi Ningshen oral liquid on GABA and Glu contents in brain tissue of rats[J]. Journal of Clinical Medicine Practice, 2008, 1(16): 739-740.
[29]	桂丽, 章功良, 张景行, 等. 兴奋和损毁外侧缰核对大鼠睡眠觉醒周期的影响[J]. 安徽医科大学学报,2006(5):511−513. [GUI L, ZHANG G L, ZHANG J X, et al. The effect of excitement and damage to the lateral habenula on sleep and wake cycle in rats[J]. Journal of Anhui Medical University,2006(5):511−513. doi: 10.3969/j.issn.1000-1492.2006.05.009 GUI L, ZHANG G L, ZHANG J X, et al. The effect of excitement and damage to the lateral habenula on sleep and wake cycle in rats[J]. Journal of Anhui Medical University, 2006(5): 511-513. doi: 10.3969/j.issn.1000-1492.2006.05.009
[30]	李求实, 王升旭. REM睡眠剥夺后大鼠脑组织氨基酸类神经递质含量的变化[J]. 华南国防医学杂志,2002(3):1−3. [LI Q S, WANG S X. Changes in the content of amino acid neurotransmitters in the brain tissue of rats after REM sleep deprivation[J]. South China Medical Journal of National Defence,2002(3):1−3. doi: 10.3969/j.issn.1009-2595.2002.03.001 LI Q S, WANG S X. Changes in the content of amino acid neurotransmitters in the brain tissue of rats after REM sleep deprivation[J]. South China Medical Journal of National Defence, 2002(3): 1-3. doi: 10.3969/j.issn.1009-2595.2002.03.001
[31]	MODIRROUSTA, M, L MAINVILE, B E J. Gabaergic neurons with α2-adrenergic receptors in basal forebrain and preoptic area express c-Fos during sleep. Neuroscience, 2004. 129(3): 803-810.
[32]	WATSON C J, LYDIC R, BAGHDOYAN H A. Sleep and GABA levels in the oral part of rat pontine reticular formation are decreased by local and systemic administration of morphine[J]. Neuroscience, 2007, 144(1):375-386.