Analysis of Active Constituents, Antioxidant and Enzyme Inhibitory Activities of <i>Epimedium sagittatum</i> Extract

WANG Qinjun; WANG Cuiping; LIU Jianxin; CHEN Chen

doi:10.13386/j.issn1002-0306.2021100171

Volume 43 Issue 12

Jun. 2022

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Science and Technology of Food Industry > 2022 > 43(12): 354-360. > DOI: 10.13386/j.issn1002-0306.2021100171

WANG Qinjun, WANG Cuiping, LIU Jianxin, et al. Analysis of Active Constituents, Antioxidant and Enzyme Inhibitory Activities of Epimedium sagittatum Extract[J]. Science and Technology of Food Industry, 2022, 43(12): 354−360. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100171.

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Analysis of Active Constituents, Antioxidant and Enzyme Inhibitory Activities of Epimedium sagittatum Extract

1.
College of Biological Science and Engineering, Shaanxi University of Science and Technology/Shaanxi Key Laboratory of Resource Biology/Collaborative Innovation Center for Comprehensive Development of Biological Resources in Qinba Mountain area of Southern Shaanxi/Key Laboratory of Qinba Biological Resources and Ecological Environment of Shaanxi Province (Cultivation), Hanzhong 723000, China
2.
Hanzhong Natural Valley Biotechnology Co., Ltd., Hanzhong 723000, China

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Received Date: October 17, 2021
Available Online: April 14, 2022

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Abstract

Objective: To study and evaluate the active components, contents and in vitro biological activities of Epimedium sagittatum extract. Methods: Contents of total polyphenols, total flavonoids, total polysaccharides and proanthocyanidins in the extract of Epimedium sagittatum were determined by spectrophotometry, the contents of five flavonoids in the extract were determined by high performance liquid chromatography (HPLC), the antioxidant activity of Epimedium sagittatum extract was evaluated by scavenging DPPH, OH, ABTS⁺ free radical method, and the inhibitory effects on pancreatic lipase, tyrosinase, acetylcholinesterase and butyrylcholinesterase were determined. Results: The contents of total polyphenols, total flavonoids, total polysaccharides and proanthocyanidins in the extract of Epimedium sagittatum were 105.60±0.92, 70.09±0.75, 18.45±1.27, 8.79±0.13 mg/g, respectively. HPLC detected five kinds of flavonoids in the extract. Among them, Icariin and epimedin C contained the highest content, which were 266.16±4.22 and 43.35±0.67 mg/g, respectively. Baohuoside I contained the least, which was 2.54±0.07 mg/g. Epimedium sagittatum extracts had certain free radical scavenging ability. The half-clearing concentrations (IC₅₀) of Epimedium sagittatum extracts on DPPH·, ·OH, and ABTS⁺· were 4.43±0.40, 2.83±0.09 and 2.04±0.08 mg/mL, respectively. The scavenging ability was concentration-dependent. Meanwhile, The IC₅₀ of Epimedium sagittatum extract on lipase, tyrosinase and acetyl/butyrylcholinesterase were 5.97±0.04, 2.27±0.23, 9.27±0.07 and 7.41±0.26 mg/mL, respectively. Conclusion: Epimedium sagittatum extracts was rich in active substances, had a good scavenging effect on DPPH, OH, ABTS⁺ free radicals, and had certain inhibition on pancreatic lipase, tyrosinase, acetylcholinesterase and butyrylcholinesterase effect.
- Epimedium,
- active ingredients,
- content,
- free radical scavenging,
- enzyme inhibition

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References

[1]	张华峰, 杨晓华. 淫羊藿在食品工业中的应用现状与展望[J]. 食品工业科技,2010,31(5):390−393. [ZHANG H F, YANG X H. Application of herba Epimedii in food industry: Current status and prospect[J]. Science and Technology of Food Industry,2010,31(5):390−393. ZHANG H F, YANG X H. Application of herba Epimedii in food industry: current status and prospect[J]. Science and Technology of Food Industry, 2010, 31(5): 390-393.
[2]	殷涛, 李云峰, 徐宁, 等. 淫羊藿人工种植现状及思考[J]. 时珍国医国药,2020,31(6):1468−1471. [YIN T, LI Y F, XU N, et al. Current situation and thinking of artificial cultivation of Epimedium[J]. Lishizhen Medicine and Materia Medica Research,2020,31(6):1468−1471. doi: 10.3969/j.issn.1008-0805.2020.06.063 YIN T, LI Y F, XU N, et al. Current situation and thinking of artificial cultivation of Epimedium[J]. Lishizhen Medicine and Materia Medica Research, 2020, 31(6): 1468-1471. doi: 10.3969/j.issn.1008-0805.2020.06.063
[3]	孟宁, 孔凯, 李师翁. 淫羊藿属植物化学成分及药理活性研究进展[J]. 西北植物学报,2010,30(5):1063−1073. [MENG N, SUN K, LI S W, et al. Advances in studies on chemical constituents and pharmaceutical activities in species of Epimedium[J]. Acta Botanica Boreali-Occidentalia Sinica,2010,30(5):1063−1073. MENG N, SUN K, LI S W, et al. Advances in studies on chemical constituents and pharmaceutical activities in species of Epimedium[J]. Acta Botanica Boreali-Occidentalia Sinica, 2010, 30(5): 1063-1073.
[4]	欧阳慧子, 何俊. 淫羊藿化学成分分析及其药代动力学研究进展[J]. 天津中医药大学学报,2019,38(3):219−227. [OUYANG H Z, HE J. Research progress on the quantitative analysis of chemical constituents in Epimedium herb and its pharmacokinetics[J]. Journal of Tianjin University of Traditional Chinese Medicine,2019,38(3):219−227. OUYANG H Z, HE J. Research progress on the quantitative analysis of chemical constituents in Epimedium herb and its pharmacokinetics[J]. Journal of Tianjin University of Traditional Chinese Medicine, 2019, 38(3): 219-227.
[5]	ZHANG H, WANG H, WEI J, et al. Comparison of the active compositions between raw and processed Epimedium from different species[J]. Molecules,2018,23(7):1656. doi: 10.3390/molecules23071656
[6]	袁航, 曹树萍, 陈抒云, 等. 淫羊藿的化学成分及质量控制研究进展[J]. 中草药,2014,45(24):3630−3640. [YUAN H, CAO S P, CHEN S Y, et al. Research progress on chemical constituents and quality control of Epimedii folium[J]. Chinese Traditional and Herbal Drugs,2014,45(24):3630−3640. doi: 10.7501/j.issn.0253-2670.2014.24.024 YUAN H, CAO S P, CHEN S Y, et al. Research progress on chemical constituents and quality control of Epimedii Folium[J]. Chinese Traditional and Herbal Drugs, 2014, 45(24): 3630-3640. doi: 10.7501/j.issn.0253-2670.2014.24.024
[7]	付亮, 袁璟亚, 杨瑞武, 等. 正交试验优化淫羊藿总黄酮和多糖的分步提取工艺优化[J]. 食品科学,2012,33(24):56−60. [FU L, YUAN J Y, YANG R W, et al. Orthogonal array design for the optimization of successive extraction of total flavonoids and polysaccharides from Epimedium brevicornu Maxim[J]. Food Science,2012,33(24):56−60. doi: 10.7506/spkx1002-6630-201224012 FU L, YUAN J Y, YANG R W, et al. Orthogonal array design for the optimization of successive extraction of total flavonoids and polysaccharides from Epimedium brevicornu Maxim[J]. Food Science, 2012, 33(24): 56-60. doi: 10.7506/spkx1002-6630-201224012
[8]	国家药典委员会. 中华人民共和国药典: 一部[M]. 北京: 中国医药科技出版社, 2020: 340 National Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China: Part one [M]. Beijing: China Medical Science and Technology Press, 2020: 340.
[9]	徐艳琴, 李仁清, 张华雁, 等. 箭叶淫羊藿的资源分布及质量特征研究进展[J]. 中草药,2020,51(23):6119−6132. [XU Y Q, LI R Q, ZHANG Y H, et al. Distribution of Epimedium sagittatum and its research progress on quality characteristics[J]. Chinese Traditional and Herbal Drugs,2020,51(23):6119−6132. doi: 10.7501/j.issn.0253-2670.2020.23.027 XU Y Q, LI R Q, ZHANG Y H, et al. Distribution of Epimedium sagittatum and its research progress on quality characteristics[J]. Chinese Traditional and Herbal Drugs, 2020, 51(23): 6119-6132. doi: 10.7501/j.issn.0253-2670.2020.23.027
[10]	侯旭东, 胡情, 马丽娟, 等. 源于中药的胰脂肪酶抑制剂研究进展[J]. 药学学报,2020,55(7):1478−1493. [HOU X D, HU Q, MA L J, et al. Recent advances on pancreatic lipase inhibitors from Chinese herbs[J]. Acta Pharmaceytica Sinica,2020,55(7):1478−1493. HOU X D, HU Q, MA L J, et al. Recent advances on pancreatic lipase inhibitors from Chinese herbs[J]. Acta Pharmaceytica Sinica, 2020, 55(7): 1478-1493.
[11]	马正月, 张仕国, 张元功, 等. 硫色满-4-酮类乙酰胆碱酯酶抑制剂的设计、合成及活性评价[J]. 中国药学杂志,2013,48(20):1774−1778. [MA Z Y, ZHANG S G, ZHANG Y G, et al. Design, synthesis and evaluation of thiochroman-4-ones as acetylcholinesterase inhibitors[J]. Chinese Pharmaceytica Journal,2013,48(20):1774−1778. MA Z Y, ZHANG S G, ZHANG Y G, et al. Design, synthesis and evaluation of Thiochroman-4-ones as acetylcholinesterase inhibitors[J]. Chinese Pharmaceytica Journal, 2013, 48(20): 1774-1778.
[12]	毕云枫, 宋凤瑞, 刘志强. 天然酪氨酸酶抑制剂的种类及其对酪氨酸酶抑制作用的研究进展[J]. 吉林大学学报(医学版),2014,40(2):454−459. [BI Y F, SONG F R, LIU Z Q, et al. Research progress on types of natural tyrosinase inhibitors and their inhibitory effects on tyrosinase[J]. Journal of Jilin University (Medicine Edition),2014,40(2):454−459. BI Y F, SONG F R, LIU Z Q, et al. Research progress on types of natural tyrosinase inhibitors and their inhibitory effects on tyrosinase[J]. Journal of Jilin University(Medicine Edition), 2014, 40(2): 454-459.
[13]	谭莉, 陈瑞战, 常清泉, 等. 淫羊藿叶多糖工艺优化及抗氧化活性[J]. 食品科学,2017,38(2):255−263. [TAN L, CHEN R Z, CHANG Q Q, et al. Optimization of extraction and antioxidant activity of polysaccharides from Epimedium leaves[J]. Food Science,2017,38(2):255−263. doi: 10.7506/spkx1002-6630-201702040 TAN L, CHEN R Z, CHANG Q Q, et al. Optimization of extraction and antioxidant activity of polysaccharides from Epimedium leaves[J]. Food Science, 2017, 38(2): 255-263. doi: 10.7506/spkx1002-6630-201702040
[14]	席晓志, 崔晓伟, 郭弘, 等. 响应面分析法优化后淫羊藿提取物抗氧化活性研究[J]. 辽宁中医药大学学报,2018,20(4):63−67. [XI X Z, CUI X W, GUO H, et al. Research of response surface methodology for optimization on extraction conditions of antioxidant activity from Epimedium brevicornu Maxim[J]. Journal of Liaoning University of Traditional Chinese Medicine,2018,20(4):63−67. XI X Z, CUI X W, GUO H, et al. Research of response surface methodology for optimization on extraction conditions of antioxidant activity from Epimedium Brevicornu Maxim[J]. Journal of Liaoning University of Traditional Chinese Medicine, 2018, 20(4): 63-67.
[15]	张运辉, 周小青, 伍大华, 等. 基于网络药理学的淫羊藿治疗阿尔茨海默病作用机制研究[J]. 中国现代应用药学,2021,38(16):1945−1951. [ZHANG Y H, ZHOU X Q, WU D H, et al. Study on the mechanism of Epimedii folium in treating Alzheimer’s disease based on network pharmacology[J]. Chinese Journal of Modern Applied Pharmacy,2021,38(16):1945−1951. ZHANG Y H, ZHOU X Q, WU D H, et al. Study on the mechanism of Epimedii Folium in treating Alzheimer’s disease based on network pharmacology[J]. Chinese Journal of Modern Applied Pharmacy, 2021, 38(16): 1945-1951.
[16]	WANG D, XU W, CHEN X, et al. Icariin induces cell differentiation and cell cycle arrest in mouse melanoma B16 cells via Erk1/2-p38-JNK-dependent pathway[J]. Oncotarget,2017,8(59):99504−99513. doi: 10.18632/oncotarget.20118
[17]	WANG X L, WANG N, ZHENG L Z, et al. Phytoestrogenic molecule desmethylicaritin suppressed adipogenesis via Wnt/β-catenin signaling pathway[J]. European Journal of Pharmacology,2013,714(1−3):254−260.
[18]	BAHADORI M B, VALIZADEH H, ASGHARI B, et al. Chemical composition and antimicrobial, cytotoxicity, antioxidant and enzyme inhibitory activities of Salvia spinosa L.[J]. Journal of Functional Foods,2015,18:727−736.
[19]	王平, 李雪松, 任晓峰, 等. 一种从淫羊藿属植物制备高含量(98%)淫羊藿甙的技术: 中国, 102807595A [P]. 2012-12-05. WANG P, LI X S, REN X F, et al A method for preparing high content Epimedium from Epimedium plants Technology of icariin (98%): China, 102807595a [P]. 2012-12-05.
[20]	AKTUMSEK A, ZENGING, GULER G O, et al. Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species[J]. Food and Chemical Toxicology,2013,55:290−296. doi: 10.1016/j.fct.2013.01.018
[21]	XI W P, LU J F, QUN J P, et al. Characterization of phenolic profile and antioxidant capacity of different fruit part from lemon (Citrus limon Burm.) cultivars[J]. Journal of Food Science and Technology,2017,54(5):1108−1118. doi: 10.1007/s13197-017-2544-5
[22]	陈琛, 李鑫鑫, 徐尤美, 等. 天麻多糖的分离纯化与抗氧化活性研究[J]. 中国临床药理学杂志,2018,34(18):2203−2206. [CHEN C, LI X X, XU Y M, et al. Extraction, purification and antioxidant activity of polysaccharides from Gastrodia elata B1[J]. The Chinese Journal of Clinical Pharmacology,2018,34(18):2203−2206. CHEN C, LI X X, XU Y M, et al. Extraction, purification and antioxidant activity of polysaccharides from Gastrodia elata B1[J]. The Chinese Journal of Clinical Pharmacology, 2018, 34(18): 2203-2206.
[23]	石磊, 高哲, 刘丽南, 等. 四种原花青素含量测定方法比较[J]. 食品工业科技,2019,40(15):242−247,253. [SHI L, GAO Z, LIU L N, et al. Comparison of four methods for quantitation of proanthocyanidin[J]. Science and Technology of Food Industry,2019,40(15):242−247,253. SHI L, GAO Z, LIU L N, et al. Comparison of four methods for quantitation of proanthocyanidin[J]. Science and Technology of Food Industry, 2019, 40(15): 242-247, 253.
[24]	王燕萍, 贾旭森, 王艳, 等. 不同干燥方式对淫羊藿黄酮类成分含量及抗氧化活性影响[J]. 中草药,2021,52(14):4193−4200. [WANG Y P, JIA X S, WANG Y, et al. Effects of different drying methods on flavonoids content and antioxidant activity of Epimedii folium[J]. Chinese Traditional and Herbal Drugs,2021,52(14):4193−4200. doi: 10.7501/j.issn.0253-2670.2021.14.009 WANG Y P, JIA X S, WANG Y, et al. Effects of different drying methods on flavonoids content and antioxidant activity of Epimedii Folium[J]. Chinese Traditional and Herbal Drugs, 2021, 52(14): 4193-4200. doi: 10.7501/j.issn.0253-2670.2021.14.009
[25]	CHEN X W, QI Y J, ZHU C H, et al. Effect of ultrasound on the properties and antioxidant activity of hawthorn pectin[J]. International Journal of Biological Macromolecules,2019,131:273−281. doi: 10.1016/j.ijbiomac.2019.03.077
[26]	ZHAO Y, DU S K, WANG H X, et al. In vitro antioxidant activity of extracts from common legumes[J]. Food Chemistry,2014,152:462−466. doi: 10.1016/j.foodchem.2013.12.006
[27]	ROZI P, ABUDUWAILI A, MUTAILIFU P, et al. Sequential extraction, characterization and antioxidant activity of polysaccharides from Fritillaria pallidiflora Schrenk[J]. International Journal of Biological Macromolecules,2019,131:97−106. doi: 10.1016/j.ijbiomac.2019.03.029
[28]	李鑫鑫, 王一棠, 陈琛, 等. 12种植物粗多糖的体外抗肥胖及抗氧化活性研究[J]. 食品科技,2018,43(2):222−227. [LI X X, WANG Y T, CHEN C, et al. The anti-obesity and anti-oxidant activities of 12 crude polysaccharide[J]. Food Science and Technology,2018,43(2):222−227. LI X X, WANG Y T, CHEN C, et al. The anti-obesity and anti-oxidant activities of 12 crude polysaccharide[J]. Food Science and Technology, 2018, 43(2): 222-227.
[29]	袁晓擘, 李世刚, 张宏岐, 等. 五峰绿茶的体外抗氧化活性研究[J]. 食品研究与开发,2019,40(3):51−55. [YUAN X B, LI S G, ZHANG H Q, et al. Study on antioxidative activity of total extracted from Wu-feng green tea in vitro[J]. Food Research and Development,2019,40(3):51−55. doi: 10.3969/j.issn.1005-6521.2019.03.009 YUAN X B, LI S G, ZHANG H Q, et al. Study on antioxidative activity of total extracted from Wu-feng green tea in vitro[J]. Food Research and Development, 2019, 40(3): 51-55. doi: 10.3969/j.issn.1005-6521.2019.03.009
[30]	ORHAN I E, SENOL F S, OZTURK N, et al. Phytochemical contents and enzyme inhibitory and antioxidant properties of Anethum graveolens L. (Dill) samples cultivated under organic and conventional agricultural conditions[J]. Food and Chemical Toxicology,2013,59:96−103. doi: 10.1016/j.fct.2013.05.053
[31]	廖莉玲, 刘程程, 韦万丽, 等. 7种黔产补益类中草药抗氧化成分的测定[J]. 时珍国医国药,2012,23(10):2498−2500. [LIAO L L, LIU C C, WEI W L, et al. Determination of the contents of main antioxidant compositions in seven Chinese tonic herbs collected in Guizhou province[J]. Lishizhen Medicine and Materia Medica Research,2012,23(10):2498−2500. doi: 10.3969/j.issn.1008-0805.2012.10.048 LIAO L L, LIU C C, WEI W L, et al. Determination of the contents of main antioxidant compositions in seven Chinese tonic herbs collected in Guizhou province[J]. Lishizhen Medicine and Materia Medica Research, 2012, 23(10): 2498-2500. doi: 10.3969/j.issn.1008-0805.2012.10.048
[32]	李倩, 李晓, 魏悦, 等. 淫羊藿总黄酮的碱提工艺研究[J]. 天然产物研究与开发,2014,26(7):1141−1144. [LI Q, LI X, WEI Y, et al. Extraction of total flavonoid from herba Epimedii maximusing alkali solution[J]. Natural Product Research and Development,2014,26(7):1141−1144. LI Q, LI X, WEI Y, et al. Extraction of total flavonoid from herba Epimedii Maximusing alkali solution[J]. Natural Product Research and Development, 2014, 26(7): 1141-1144.
[33]	付亮, 袁璟亚, 丁春邦, 等. 淫羊藿药渣中多糖的提取及其体外抗氧化活性评价[J]. 食品工业科技,2016,37(1):116−120. [FU L, YUAN J Y, DING C B, et al. Polysaccharides extraction from residue of Epimedium and its antioxidant activity evaluation[J]. Science and Technology of Food Industry,2016,37(1):116−120. FU L, YUAN J Y, DING C B, et al. Polysaccharides extraction from residue of Epimedium and its antioxidant activity evaluation[J]. Science and Technology of Food Industry, 2016, 37(1): 116-120.
[34]	党亚锋, 袁洪超, 张圣文, 等. 巫山淫羊藿不同药用部位有效成分分析研究[J]. 山东化工,2021,50(14):107−108. [DANG Y F, YUAN H C, ZHANG S W, et al. Analysis and study on the effective components of different parts of Epimedium Wushan[J]. Shandong Chemical Industry,2021,50(14):107−108. doi: 10.3969/j.issn.1008-021X.2021.14.043 DANG Y F, YUAN H C, ZHANG S W, et al. Analysis and study on the effective components of different parts of Epimedium Wushan[J]. Shandong Chemical Industry, 2021, 50(14): 107-108. doi: 10.3969/j.issn.1008-021X.2021.14.043
[35]	张翔, 张华峰. 淫羊藿黄酮的超高压提取工艺研究[J]. 食品科技,2019,44(4):250−254. [ZHANG X, ZHANG H F. Optimization of flavonoids extraction from Epimedium sagittatum by ultra high pressure extraction[J]. Food Science and Technology,2019,44(4):250−254. ZHANG X, ZHANG H F. Optimization of flavonoids extraction from Epimedium sagittatum by ultra high pressure extraction[J]. Food Science and Technology, 2019, 44(4): 250-254.
[36]	于雪娥, 秦建平, 李家春, 等. 一测多评法同时测定淫羊藿总黄酮胶囊中7种黄酮类成分[J]. 中国实验方剂学杂志,2017,23(7):79−85. [YU X E, QIN J P, LI J C, et al. Simultaneous determination of 7 flavonoid compounds Yinyanghuo zonghuangtong capsule by quantitative analysis of multi-components with a single-marker[J]. Chinese Journal of Experimental Traditional Medical Formulae,2017,23(7):79−85. YU X E, QIN J P, LI J C, et al. Simultaneous determination of 7 flavonoid compounds Yinyanghuo zonghuangtong capsule by quantitative analysis of multi-components with a single-marker[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2017, 23(7): 79-85.
[37]	杨娟, 张华峰, 牛丽丽, 等. 不同地区商品淫羊藿中朝藿定、淫羊藿苷、总黄酮含量变异及抗氧化活性分析[J]. 食品工业科技,2014,35(16):96−100. [YANG J, ZHANG H F, NIU L L, et al. Variation of epimedins, icariin and total flavonoids and evaluation of antioxidant activities of Epimedii folium in different areas of China[J]. Science and Technology of Food Industry,2014,35(16):96−100. YANG J, ZHANG H F, NIU L L, et al. Variation of epimedins, icariin and total flavonoids and evaluation of antioxidant activities of Epimedii Folium in different areas of China[J]. Science and Technology of Food Industry, 2014, 35(16): 96-100.
[38]	ZHAO Y, CHEN S, WANG Y, et al. Effect of drying processes on prenylflavonoid content and antioxidant activity of Epimedium koreanum Nakai[J]. J Food Drug Anal,2018,26(2):796−806. doi: 10.1016/j.jfda.2017.05.011

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