Effects of Walnut Pulp on the Cultivation Characteristics and Anti-hypoxia Potential of <i>Lactobacillus rhamnosus</i> Enriched with Selenium

LI Yinghui; XU Fengqin; LIU Guanwen; QU Wenjuan; MA Haile; Aniqa ZIA; SHI Junling

doi:10.13386/j.issn1002-0306.2021100162

Volume 43 Issue 14

Jul. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(14): 127-137. > DOI: 10.13386/j.issn1002-0306.2021100162

LI Yinghui, XU Fengqin, LIU Guanwen, et al. Effects of Walnut Pulp on the Cultivation Characteristics and Anti-hypoxia Potential of Lactobacillus rhamnosus Enriched with Selenium[J]. Science and Technology of Food Industry, 2022, 43(14): 127−137. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100162.

Citation:

PDF (4387 KB)

Effects of Walnut Pulp on the Cultivation Characteristics and Anti-hypoxia Potential of Lactobacillus rhamnosus Enriched with Selenium

1.
School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, China
2.
Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China

More Information

Received Date: October 17, 2021
Available Online: May 07, 2022

Graphical Abstract

Abstract

Abstract

In order to develop a cheap and efficient culture system of Lactobacillus rhamnosus, walnut pulp was used to replace the nitrogen source in MRS medium. Taking cell growth as the indicator, the cultivation system was optimized in aspects of walnut pulp particle size, solid-liquid ratio, dosage, lipid removing treatment, and the shaking speed during the cultivation of Lactobacillus rhamnosus. The antibacterial activity and antioxidant activity of cell-free culture supernatant were also tested in all conditions. The obtained cells were also subjected to selenium enrichment treatment and then fed to mice for 3 d before they were exposed to simulated low-pressure hypoxia condition (altitude 5000 m) for 6 h to evaluate their anti-hypoxia potential. As results, complete replacement of the nitrogen source (beef extract and peptone) of MRS medium with the lipid-free walnut pulp filtered by 120 mesh screen at the solid-liquid ratio of 1:10 (g/mL) could reduce the cost of MRS medium by 70% and increase the cell yield by 2.5 times (from 2.6×10⁹ to 7.6×10⁹ CFU/mL), but did not affect the antibacterial activity and antioxidant activity of the fermentation broth (P>0.05). Continuous oral administration of selenium enriched bacteria for 3 days could significantly increase the hemoglobin content of mice when they were exposed to low-pressure hypoxia for 6 h (P<0.05), showing better effects than that cultivated in MRS medium. In conclusion, walnut pulp could be used to replace the nitrogen source in MRS medium for the culture of Lactobacillus rhamnosus, and effectively improve the cell growth and activity.
- Lactobacillus rhamnosus,
- selenium-enriched bacterial cells,
- walnut,
- nitrogen resource,
- liquid cultivation

FullText(HTML)

References (42)

References

[1]	QU Rui, JIANG Chunmei, WU Wanqin, et al. Conversion of DON to 3-epi-DON in vitro and toxicity reduction of DON in vivo by Lactobacillus rhamnosus[J]. Food & Function,2019,10(5):2785−2796.
[2]	LI Na, PANG Bing, LI Junjun, et al. Mechanisms for Lactobacillus rhamnosus treatment of intestinal infection by drug-resistant Escherichia coli[J]. Food & Function,2020,11(5):4428−4445.
[3]	LI Na, PANG Bing, LIU Guanwen, et al. Lactobacillus rhamnosus from human breast milk shows therapeutic function against foodborne infection by multi-drug resistant Escherichia coli in mice[J]. Food & Function,2020,11(1):435−477.
[4]	LIU Guanwen, PANG Bing, LI Na, et al. Therapeutic effect of Lactobacillus rhamnosus SHA113 on intestinal infection by multi-drug-resistant Staphylococcus aureus and its underlying mechanisms[J]. Food & Function,2020,11(7):6226−6239.
[5]	李晓军, 马跃英, 郭跃东, 等. 响应面法优化保加利亚乳杆菌发酵培养基的氮源[J]. 农产品加工,2020,4(22):56−59. [LI X J, MA Y Y, GUO Y D, et al. Optimization of nitrogen sources for Lactobacillus bulgaricus fermentation medium by response surface methodology[J]. Farm Products Processing,2020,4(22):56−59. LI X J, MA Y Y, GUO Y D, et al. Optimization of nitrogen sources for Lactobacillus bulgaricus fermentation medium by response surface methodology[J]. Farm Products Processing, 2020, 4(22): 56-59.
[6]	丛美楠. 保加利亚乳杆菌的低成本培养、保藏及初步应用研究[D]. 厦门: 集美大学, 2017 CONG M N. Low-cost culture, preservation and preliminary study of Lactobacillus bulgaricus[D]. Xiamen: Jimei University, 2017.
[7]	曹炎. 低成本乳酸菌培养基筛选及其发酵菌液对稻草发酵效果[D]. 延边: 延边大学, 2012 CAO Y. The selection of production Lactobacillus at low price and the effect of fermented straw[D]. Yanbian: Yanbian University, 2012.
[8]	邓楚津, 赖丽芬, 曾少葵, 等. 乳酸菌利用罗非鱼下脚料水解液发酵L-乳酸的探讨[J]. 南方水产科学,2012,8(1):61−66. [DENG C J, LAI L F, ZENG S K, et al. Research on L-lactic acid fermentation for hydrolysate of tilapia wastes with Lactobacillus[J]. South China Fisheries Science,2012,8(1):61−66. doi: 10.3969/j.issn.2095-0780.2012.01.010 DENG C J, LAI L F, ZENG S K, et al. Research on L-lactic acid fermentation for hydrolysate of tilapia wastes with Lactobacillus[J]. South China Fisheries Science, 2012, 8(1): 61-66. doi: 10.3969/j.issn.2095-0780.2012.01.010
[9]	MICHALCZYK A K, GARBACZEWSKA S, MORYTZ B, et al. Influence of nitrogen sources on D-lactic acid biosynthesis by Sporolactobacillus laevolacticus DSM 442 strain[J]. Fermentation-Basel,2021,7(2):78. doi: 10.3390/fermentation7020078
[10]	POLAK-BERECKA M, WASKO A, KORDOWSKA-WIATER M, et al. Application of response surface methodology to enhancement of biomass production by Lactobacillus rhamnosus E/N[J]. Brazilian Journal of Microbiology,2011,42(4):1485−1494. doi: 10.1590/S1517-83822011000400035
[11]	WU Wanqin, PANG Bing, YANG Rongrong, et al. Improvement of the probiotic potential and yield of Lactobacillus rhamnosus cells using corn steep liquor[J]. LWT,2020,131:109862. doi: 10.1016/j.lwt.2020.109862
[12]	RAJA V, AHMADS I, IRSHADM, et al. Anticandidal activity of ethanolic root extract of Juglans regia (L.): Effect on growth, cell morphology, and key virulence factors[J]. Journal De Mycologie Medicale,2017,27(4):476−486. doi: 10.1016/j.mycmed.2017.07.002
[13]	ZHOU Hongxiang, LIU Yin, TIAN Yali, et al. Optimization of the enzymolysis technology of walnut protein by trypsin using response surface methodology[J]. Medicinal Plant,2014(Z1):19−23.
[14]	刘丹丹. 核桃蛋白的抗氧化与降血压活性评价及酶解必要性研究[D]. 镇江: 江苏大学, 2020 LIU D D. Antioxidant and antihypertensive activities evaluation and the enzymatic hydrolysisnecessity of walnut protein[D]. Zhenjiang: Jiangsu University, 2020.
[15]	杨家军, 黄克和, 赵治平, 等. 富硒益生菌对小鼠生长、抗氧化力和肠道菌群的影响[J]. 食品科学,2009,30(1):242−245. [YANG J J, HUANG K H, ZHAO Z P, et al. Effects of selenium-enriched probiotics on growth performance, anti-oxidant status and resident intestinal flora in Kunming mice[J]. Food Science,2009,30(1):242−245. doi: 10.3321/j.issn:1002-6630.2009.01.057 YANG J J, HUANG K H, ZHAO Z P, et al. Effects of selenium-enriched probiotics on growth performance, anti-oxidant status and resident intestinal flora in Kunming mice[J]. Food Science, 2009, 30(1): 242-245. doi: 10.3321/j.issn:1002-6630.2009.01.057
[16]	许牡丹, 贺佳璇, 徐颖, 等. 七株富硒乳酸菌的功能特性评价及其抗氧化活性[J]. 陕西科技大学学报,2020,38(4):31−37. [XU M D, HE J X, XU Y, et al. Evaluation of functional characteristics and antioxidant activity of seven strains of selenium-enriched lactic acid bacteria[J]. Journal of Shaanxi University of Science & Technology,2020,38(4):31−37. doi: 10.3969/j.issn.1000-5811.2020.04.006 XU M D, HE J X, XU Y, et al. Evaluation of functional characteristics and antioxidant activity of seven strains of selenium-enriched lactic acid bacteria[J]. Journal of Shaanxi University of Science & Technology, 2020, 38(4): 31-37. doi: 10.3969/j.issn.1000-5811.2020.04.006
[17]	程远之. 富硒芽孢杆菌SR14的筛选、性状表征及其抗氧化功能与机制探究[D]. 杭州: 浙江大学, 2020 CHENG Y Z. Screening, characterization, antioxidant function and mechanism of selenium-rich Bacillus SR14[D]. Hangzhou: Zhejiang University, 2020.
[18]	LIU Lei, CHEN Daiwen, YU Bing, et al. Influences of selenium-enriched yeast on growth performance, immune function, and antioxidant capacity in weaned pigs exposure to oxidative stress[J]. BioMed Research International,2021,2021:5533210.
[19]	HAMID M, ABDULRAHIM Y, LIU D D, et al. Selenium enriched yeast and gum arabic combination attenuate oxidative liver damage via suppression of oxidative stress, inhibition of caspase-3 and pro-inflammatory genes expression in carbon tetrachloride-intoxicated rats[J]. Bioactive Carbohydrates and Dietary Fibre,2021(26):100267.
[20]	徐颖, 杨玉洁, 何成艳, 等. 盐胁迫鼠李糖乳杆菌富硒工艺研究[J]. 陕西科技大学学报,2020,38(1):58−63. [XU Y, YANG Y J, HE C Y, et al. Study on selenium enrichment process of Lactobacillus rhamnosus under salt stress[J]. Journal of Shaanxi University of Science & Technology,2020,38(1):58−63. doi: 10.3969/j.issn.1000-5811.2020.01.010 XU Y, YANG Y J, HE C Y, et al. Study on selenium enrichment process of Lactobacillus rhamnosus under salt stress[J]. Journal of Shaanxi University of Science & Technology, 2020, 38(1): 58-63. doi: 10.3969/j.issn.1000-5811.2020.01.010
[21]	RAJOKA M S R, MEHWISH H M, SIDDIQ M, et al. Identification, characterization, and probiotic potential of Lactobacillus rhamnosus isolated from human milk[J]. LWT-Food Science and Technology,2017,84:271−280. doi: 10.1016/j.lwt.2017.05.055
[22]	TULUMOGLU S, YUKSEKDAG Z N, BEYATLI Y, et al. Probiotic properties of Lactobacilli species isolated from children's feces[J]. Anaerobe,2013,24:36−42. doi: 10.1016/j.anaerobe.2013.09.006
[23]	CHOORUK A, PIWAT S, TEANPAISAN R. Antioxidant activity of various oral Lactobacillus strains[J]. Journal of Applied Microbiology,2017,123(1):271−279. doi: 10.1111/jam.13482
[24]	陈庭木, 孙志广, 邢运高, 等. 水稻可消化蛋白质含量测定方法研究及资源筛选[J]. 作物杂志,2020(1):61−66. [CHEN T M, SUN Z G, XING Y G, et al. Determination of digestible protein content in rice and resource screening[J]. Crops,2020(1):61−66. CHEN T M, SUN Z G, XING Y G, et al. Determination of digestible protein content in rice and resource screening[J]. Crops, 2020(1): 61-66.
[25]	蔡红梅, 田子玉. 苯酚-硫酸法测定草莓中总糖含量[J]. 吉林农业,2019(4):46. [CAI H M, TIAN Z Y. Determination of total sugar in strawberry by phenol-sulfuric acid method[J]. Agriculture of Jilin,2019(4):46. CAI H M, TIAN Z Y. Determination of total sugar in strawberry by phenol-sulfuric acid method[J]. Agriculture of Jilin, 2019(4): 46.
[26]	国家食品药品监督管理总局, 国家卫生和计划生育委员会. GB 5009.93-2017 食品安全国家标准食品中硒的测定[S]. 北京: 中国标准出版社, 2021 State Food and Drug Administration, National Health and Family Planning Commission. GB 5009.93-2017 National food safety standard. Determination of selenium in food[S]. Beijing: China Standards Press, 2021.
[27]	吴艳丽, 刘朋, 苏咏欣, 等. 嗜黏蛋白阿克曼氏菌ATCC BAA-835肠道益生作用的体外评价[J/OL]. 食品与发酵工业: 1−10. https://doi.org/10.13995/j.cnki.11-1802/ts.027445. WU Y L, LIU P, SU Y X, et al. Evaluation of the intestinal probiotic effect of Akkermansia muciniphila ATCC BAA-835 in vitro[J/OL]. Food and Fermentation Industries: 1−10. https://doi.org/10.13995/j.cnki.11-1802/ts.027445.
[28]	ZENG H, UMAR S, RUST B, et al. Secondary bile acids and short chain fatty acids in the colon: A focus on colonic microbiome, cell proliferation, inflammation, and cancer[J]. International Journal of Molecular Sciences,2019,20(5):12−14.
[29]	LIU Yuntao, LI Yiwen, KE Yu, et al. In vitro saliva-gastrointestinal digestion and fecal fermentation of Oudemansiella radicata polysaccharides reveal its digestion profile and effect on the modulation of the gut microbiota[J]. Carbohydrate Polymers,2021,251:117041. doi: 10.1016/j.carbpol.2020.117041
[30]	郑雨星, 朱慧越, 焦婷, 等. 鼠李糖乳杆菌FZJTZ46L6通过影响肠道内短链脂肪酸的水平缓解小鼠由葡聚糖硫酸钠导致的结肠炎[J]. 食品与发酵工业,2020,46(10):44−52. [ZENG Y X, ZHU H Y, JIAO T, et al. Lactobacillus rhamnosus FZJTZ46L6 alleviates colitis induced by dextran sulfate sodium in mice through short-chain fatty acids regulation[J]. Food and Fermentation Industries,2020,46(10):44−52. ZENG Y X, ZHU H Y, JIAO T, et al. Lactobacillus rhamnosus FZJTZ46L6 alleviates colitis induced by dextran sulfate sodium in mice through short-chain fatty acids regulation[J]. Food and Fermentation Industries, 2020, 46(10): 44-52.
[31]	苏娜, 伊丽, 吉日木图. 鼠李糖乳杆菌GG发酵驼乳与牛乳的发酵特性和降糖活性比较[J]. 食品工业科技,2019,40(24):14−19,27. [SU N, YI L, JI R M T. The comparison of fermentation characteristics and hypoglycemic activity of camel milk and cow milk fermented by Lactobacillus rhamnosus GG[J]. Science and Technology of Food Industry,2019,40(24):14−19,27. SU N, YI L, JI R M T. The comparison of fermentation characteristics and hypoglycemic activity of camel milk and cow milk fermented by Lactobacillus rhamnosus GG[J]. Science and Technology of Food Industry, 2019, 40(24): 14-19, 27.
[32]	朱力杰, 方怡元, 陈妍婕, 等. 发芽糙米乳中双株复合乳酸菌的发酵特性研究[J]. 食品工业科技,2016,37(22):195−199,204. [ZHU L J, FANG Y Y, CHEN Y J, et al. Fermentation characteristics of two strains of complex lactic acid bacteria in germinated brown rice milk[J]. Science and Technology of Food Industry,2016,37(22):195−199,204. ZHU L J, FANG Y Y, CHEN Y J, et al. Fermentation characteristics of two strains of complex lactic acid bacteria in germinated brown rice milk[J]. Science and Technology of Food Industry, 2016, 37(22): 195-199, 204.
[33]	杨雪娇, 张予心, 宋秋梅, 等. 鼠李糖乳杆菌发酵大豆酸乳工艺优化研究[J]. 食品工业科技,2014,35(22):184−189. [ZHANG X J, ZHANG Y X, SONG Q M, et al. Optimization of soybean yoghurt fermentation by Lactobacillus rhamnosus[J]. Science and Technology of Food Industry,2014,35(22):184−189. ZHANG X J, ZHANG Y X, SONG Q M, et al. Optimization of soybean yoghurt fermentation by Lactobacillus rhamnosus[J]. Science and Technology of Food Industry, 2014, 35(22): 184-189.
[34]	熊传帅, 张海波, 张进明, 等. 硒和硒蛋白调节动物抗氧化功能及硒在畜牧生产中应用的研究进展[J/OL]. 中国畜牧杂志: 1−11. https://doi.org/10.13995/j.cnki.11-1802/ts.027445. XIONG C S, ZHANG H B, ZHANG J M, et al. Research progress of selenium and selenoprotein in regulating antioxidant function and application of selenium in animal production[J/OL]. Chinese Journal of Animal Science: 1−11. https://doi.org/10.13995/j.cnki.11-1802/ts.027445.
[35]	李湘利, 刘静, 朱九滨, 等. 鸡枞菌饮料的体外抗氧化特性研究[J]. 食品工业科技,2017,38(3):87−90,96. [LI X L, LIU J, ZHU J B, et al. Study on the antioxidation in vitro of Termitornyces albuminosus beverages[J]. Science and Technology of Food Industry,2017,38(3):87−90,96. LI X L, LIU J, ZHU J B, et al. Study on the antioxidation in vitro of Termitornyces albuminosus beverages[J]. Science and Technology of Food Industry, 2017, 38(3): 87-90, 96.
[36]	谢海军, 马玲, 赵玉明. 益生菌发酵乳模拟胃肠液环境下抗氧化活性[J]. 中国酿造,2017,36(5):90−94. [XIE H J, MA L, ZHAO Y M. Antioxidant activity of fermented milk with probiotics in simulated gastrointestinal fluid[J]. China Brewing,2017,36(5):90−94. doi: 10.11882/j.issn.0254-5071.2017.05.019 XIE H J, MA L, ZHAO Y M. Antioxidant activity of fermented milk with probiotics in simulated gastrointestinal fluid[J]. China Brewing, 2017, 36(5): 90-94. doi: 10.11882/j.issn.0254-5071.2017.05.019
[37]	魏铭. 增高Hb氧亲和力对血液携氧特性的影响及抗缺氧效果[D]. 重庆: 重庆医科大学, 2020 WEI M. Effect of increasing oxygen affinity of Hb on blood oxygen carrying characteristics and anti-hypoxia[D]. Chongqing: Chongqing Medical University, 2020.
[38]	林松毅, 殷涌光, 刘静波, 等. 人参等复方中药功能液对小白鼠的抗缺氧作用[J]. 吉林大学学报(工学版),2005(1):106−110. [LIN S Y, YIN Y G, LIU J B, et al. Anti-anoxic effects for mice of functional liquid from Chinese traditional compound medicines[J]. Journal of Jilin University (Engineering and Technology Edition),2005(1):106−110. LIN S Y, YIN Y G, LIU J B, et al. Anti-anoxic effects for mice of functional liquid from Chinese traditional compound medicines[J]. Journal of Jilin University (Engineering and Technology Edition), 2005(1): 106-110.
[39]	陆保革, 郭志坚, 黄慧群, 等. 3种抗缺氧药物对筑路工人急进海拔4600 m不同时间血红蛋白的影响[J]. 高原医学杂志,2007(1):10−12. [LU B G, GUO Z J, HUANG H Q, et al. Effects of three compound of anti-anoxic drugs on hemoglobin of roadman acute exposed to 4600 m altitude[J]. Journal of High Altitude Medicine,2007(1):10−12. doi: 10.3969/j.issn.1007-3809.2007.01.004 LU B G, GUO Z J, HUANG H Q, et al. Effects of three compound of anti-anoxic drugs on hemoglobin of roadman acute exposed to 4600 m altitude[J]. Journal of High Altitude Medicine, 2007(1): 10-12. doi: 10.3969/j.issn.1007-3809.2007.01.004
[40]	赵少平, 薛文敏. 高原训练对游泳运动员的影响[J]. 武汉体育学院学报,2011,45(4):78−82. [ZHAO S P, XUE W M. Effect of altitude training on swimming athletes[J]. Journal of Wuhan Institute of Physical Education,2011,45(4):78−82. doi: 10.3969/j.issn.1000-520X.2011.04.015 ZHAO S P, XUE W M. Effect of altitude training on swimming athletes[J]. Journal of Wuhan Institute of Physical Education, 2011, 45(4): 78-82. doi: 10.3969/j.issn.1000-520X.2011.04.015
[41]	HABTEZION A, NGUYEN L P, HADEIBA H, et al. Leukocyte trafficking to the small intestine and colon[J]. Gastroenterology,2016,150(2):340−354. doi: 10.1053/j.gastro.2015.10.046
[42]	MULLERW A. Getting leukocytes to the site of inflammation[J]. Veterinary Pathology,2013,50(1):7−22. doi: 10.1177/0300985812469883

Supplements (0)

Cited By

Cited by

Periodical cited type(8)

1.	韩军，王怡，张开屏，田建军. 罗伊氏粘液乳杆菌JBR3生物学特性分析及保护剂对其活力的影响. 食品工业科技. 2025(03): 166-177 . 本站查看
2.	邓忠惠，谢微. 罗汉果籽吸附氟离子效果的不同预测模型研究. 食品安全质量检测学报. 2024(06): 246-255 .
3.	刘国祎，郭建章，陈星，王威强. 响应面法和人工神经网络对亚临界CO_2萃取红花籽油的建模与优化. 食品工业科技. 2024(10): 225-233 . 本站查看
4.	马诗瑜，何敬成，詹陆川，林伟杰，林思濠，胡小刚，卞晓岚. 基于人工神经网络算法的自拟清瘟方制备工艺优化探索. 中国药业. 2023(12): 56-62 .
5.	赵清香，李大军，李亚萍，姜宇纯，李庚，袁永旭. 反向传播神经网络耦联遗传算法与响应面设计烤制鸽肉工艺优化. 中国调味品. 2023(10): 128-133 .
6.	周雷进雨，马精阳，袁月明，李锦生，冯伟志，周丽娜. 干酪乳杆菌复合冻干保护剂工艺优化. 饲料工业. 2023(22): 86-93 .
7.	渠一聪，张绍绒，罗理勇，曾亮. 基于人工神经网络耦合遗传算法（BP-GA）优化茶氨酸-葡萄糖美拉德反应的条件. 食品工业科技. 2023(24): 183-192 . 本站查看
8.	靳浩文，朱巧梅. 益生菌微胶囊技术对益生菌存活率影响的研究进展. 食品安全导刊. 2022(25): 181-183 .