Composition Differences of Procyanidins in Chestnut Shell and Pellicle before and after Digestion Based on <i>in Vitro</i> Digestion Model

LI Haonan; HUANG Xiaomin; FANG Yihe; XIAO Wenxin; ZHANG Ziqin; YANG Fang

doi:10.13386/j.issn1002-0306.2021100011

Volume 43 Issue 14

Jul. 2022

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Science and Technology of Food Industry > 2022 > 43(14): 1-9. > DOI: 10.13386/j.issn1002-0306.2021100011

LI Haonan, HUANG Xiaomin, FANG Yihe, et al. Composition Differences of Procyanidins in Chestnut Shell and Pellicle before and after Digestion Based on in Vitro Digestion Model[J]. Science and Technology of Food Industry, 2022, 43(14): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100011.

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Composition Differences of Procyanidins in Chestnut Shell and Pellicle before and after Digestion Based on in Vitro Digestion Model

1.
School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, China
2.
Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology, Wuhan 430205, China
3.
Engineering Research Center of Phosphorus Resources Development and Utilization of Ministry of Education, Wuhan 430073, China

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Received Date: October 10, 2021
Available Online: May 09, 2022

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Abstract

Abstract

In this study, the composition changes of procyanidins in chestnut shell and pellicle after simulated digestion were analyzed by extensive targeted metabolomics based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The results showed that a total of 33 procyanidin metabolites were identified in chestnut shell, pellicle and their metabolites. Differential metabolite analysis showed that 16 procyanidin compounds, including epicatechin, procyanidin C2 and procyanidin A1 were significantly (P<0.05) down-regulated in chestnut shell after digestion. Seventeen procyanidins, including gallocatechin-catechin-catechin, gallocatechin and gallocatechin gallate, were significantly (P<0.05) down-regulated in chestnut pellicle after digestion. Among them, the most significant (P<0.05) down-regulation was gallocatechin-catechin-catechin, which was reduced by 98.23% (chestnut shell) and 97.86% (chestnut pellicle) after gastrointestinal digestion in vitro. In conclusion, the stability of proanthocyanidins in chestnut shell and pellicle was poor during digestion. In future studies, targeted release carriers could be designed to encapsulate them, so as to improve their bioavailability.
- chestnut shell,
- chestnut pellicle,
- procyanidins,
- in vitro digestion,
- differential metabolites,
- metabonomics

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