GAO Shiwei, ZHU Yumeng, WANG Qilei, et al. Effects of Cold Plasma Treatment on Production and Contents of Bioactive Substances in Broccoli Sprouts[J]. Science and Technology of Food Industry, 2022, 43(12): 117−123. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090309.
Citation: GAO Shiwei, ZHU Yumeng, WANG Qilei, et al. Effects of Cold Plasma Treatment on Production and Contents of Bioactive Substances in Broccoli Sprouts[J]. Science and Technology of Food Industry, 2022, 43(12): 117−123. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090309.

Effects of Cold Plasma Treatment on Production and Contents of Bioactive Substances in Broccoli Sprouts

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  • Received Date: September 25, 2021
  • Available Online: April 14, 2022
  • In order to achieve the enrichment of sulforaphane, polyphenols, flavonoids and other bioactive substances in broccoli sprouts so as to reduce production costs, this study used plasma treatment induction method to treat broccoli sprouts. The plasma treatment conditions and its effects on the sprouts of different varieties of broccoli were studied. The results showed that the plasma treatment voltage and time had significant effects(P<0.05) on the germination state and the contents of bioactive compounds in broccoli sprouts. Under the conditions of voltage 30 kV and treatment time of 2 min, the germination rate, bud length and bud weight of broccoli sprouts after 5 days germination were significantly increased compared with the control. In addition, the sulforaphane content reached 3.24 mg/g DW, which was 6.75 times higher than that of untreated samples. There were significant differences (P<0.05) in the sulforaphane, total polyphenols, total flavonoids and antioxidant activity among different broccoli sprouts. MNL had the highest sulforaphane and glucosinolates content, while MTL and LY had the highest polyphenols and flavonoids content. After plasma treatment, WX, MNL, LY and LJ´s polyphenols and flavonoids content and antioxidation activity were all improved compared with control. In short, plasma treatment could be an effective way to enrich sulforaphane in broccoli sprouts, which would significantly improve the efficacy of sprouts and reduce the cost of sulforaphane acquisition.
  • [1]
    郭丽萍, 王志英, 杨润强, 等. 西兰花种子发芽过程中生理活性物质含量的变化[J]. 中国食品学报,2016,16(3):160−167. [GUO L, WANG Z, YANG R, et al. Changes of physiological active substances in the process of broccoli seed germination[J]. Chinese Journal of Food Science,2016,16(3):160−167.

    GUO L, WANG Z, YANG R, et al. Changes of physiological active substances in the process of broccoli seed germination[J]. Chinese Journal of Food Science, 2016, 16(3): 160-167.
    [2]
    FAHEY J W, HARISTOY X, DOLAN P M, et al. Sulforaphane inhibits extracellular, intracellular, and antibiotic-resistant strains of Helicobacter pylori and prevents benzo[a] pyrene-induced stomach tumors[J]. Proceedings of the National Academy of Sciences,2002,99(11):7610−7615. doi: 10.1073/pnas.112203099
    [3]
    TRAKA M, MITHEN R. Glucosinolates, is othiocyanates and human health[J]. Phytochemistry Reviews,2009,8(1):269−282. doi: 10.1007/s11101-008-9103-7
    [4]
    JEFFERY E H, ARAYA M. Physiological effects of broccoli consumption[J]. Phytochemistry Reviews,2008,8(1):283−298.
    [5]
    WANG G C, FARNHAM M, JEFFERY E H. Impact of thermal processing on sulforaphane yield from Broccoli (Brassica ol-eracea L. ssp. Italic)[J]. Journal of Agricultural and Food Chemistry,2012,60(27):6743−6748. doi: 10.1021/jf2050284
    [6]
    初婷, 彭畅, 郭丽萍. MgSO4处理对西兰花芽苗菜生理活性物质和抗氧化能力的影响[J]. 食品科学,2018(1):53−59. [CHU T, PENG C, GUO L, et al. Effect of MgSO4 treatment on physiological active substances and antioxidant ability of broccoli sprouts[J]. Food Science,2018(1):53−59. doi: 10.7506/spkx1002-6630-201801008

    CHU T, PENG C, GUO L, et al. Effect of MgSO4 treatment on physiological active substances and antioxidant ability of broccoli sprouts[J]. Food Science, 2018(1): 53-59. doi: 10.7506/spkx1002-6630-201801008
    [7]
    韩宇, 程雨薇, 沈敏熙, 等. 高温及其联合亚硒酸钠调控西兰花芽苗生理及异硫氰酸酯代谢研究[J]. 食品工业科技,2019,40(22):67−72. [HAN Y, CHENG Y W, SHEN M X, et al. High temperature and its combination with sodium selenite regulate broccoli sprout physiology and isothiocyanate metabolism[J]. Science and Technology of Industry,2019,40(22):67−72.

    HAN Y, CHENG Y W, SHEN M X, et al. High temperature and its combination with sodium selenite regulate broccoli sprout physiology and isothiocyanate metabolism[J]. Science and Technology of Industry. 2019, v. 40;No. 438(22): 73-78.
    [8]
    SELCUK M, OKSUZ L, BASARAN P. Decontamination of grains and legumes infected with aspergillus spp. and penicillum spp. by cold plasma treatment[J]. Bioresour. Techn.,2008,99(11):5104−5109. doi: 10.1016/j.biortech.2007.09.076
    [9]
    周芷亦, 何伟伟, 李大婧, 等. NaCl胁迫对西兰花芽苗叶黄素积累及抗氧化能力的影响[J]. 食品工业科技,2021,42(12):46−54. [ZHOU Z Y, HE W, LI D J, et al. Effects of NaCl stress on lutein accumulation and antioxidant capacity of broccoli sprouts[J]. Science and Technology of Industry,2021,42(12):46−54.

    ZHOU Z Y, HE W, LI D J, et al. Effects of NaCl stress on lutein accumulation and antioxidant capacity of broccoli sprouts[J]. Science and Technology of Industry, 2021, 42(12) : 46-54.
    [10]
    SAENGHA W, KARIRAT T, BURANRAT B, et al. Plasma technology and abiotic elicitor effectively increased isothiocyanates, bioactive compounds and cytotoxicity against Caco2 cells in mustard green microgreen extract[J]. ICoFAB2019 Proceedings, 2019.
    [11]
    KIM J W, PULIGUNDLA P, MOK C. Effect of corona discharge plasma jet on surface‐borne microorganisms and sprouting of broccoli seeds[J]. Journal of the Science of Food & Agriculture,2016:128−134.
    [12]
    吕晓桂, 王鹏, 利民, 等. 大气压氩气冷等离子体射流处理对芥菜种子发芽率及根长的影响[J]. 核聚变与等离子体物理,2019,39(2):188−192. [LÜ X G, WANG P, LI M, et al. Effect of atmospheric pressure argon cooling plasma jet treatment on the germination rate and root length of mustard seeds[J]. Nuclear Fusion and Plasma Physics,2019,39(2):188−192.

    LÜ X G, WANG P, LI M, et al. Effect of atmospheric pressure argon cooling plasma jet treatment on the germination rate and root length of mustard seeds[J]. Nuclear Fusion and Plasma Physics, 2019, 39(2): 188-192.
    [13]
    LÜ X G, MENG G L, LI W, et al. Sulforaphane and its antioxidative effects in broccoli seeds and sprouts of different cultivars[J]. Food Chemistry,2020,316:126216. doi: 10.1016/j.foodchem.2020.126216
    [14]
    李宁. 西兰花干硫苷的提取工艺优化及富集纯化研究[D]. 杭州: 浙江工商大学, 2015.

    LI N. Study on optimization of extraction process and enrichment and purification of broccoli dry glucosinolates[D]. Hangzhou: Zhejiang University of Commerce and Industry, 2015
    [15]
    吴美萍, 钟华. 等离子体浸没离子注入豌豆种子的表面形态研究[J]. 上海交通大学学报(农业科学版),1997,15(3):182−185. [WU M P, ZHONG H. Surface morphology of pea seeds implanted by plasma immersion ions[J]. Journal of Shanghai Jiao Tong University,1997,15(3):182−185.

    WU M P, ZHONG H. Surface morphology of pea seeds implanted by plasma immersion ions[J]. Journal of Shanghai Jiao Tong University. 1997, 15(3): 182-185.
    [16]
    童家赟. 空气等离子体预处理提高穿心莲种子活力的研究[D]. 广州: 广州中医药大学, 2012.

    TONG J B. Study on air plasma pretreatment to improve seed vigor of Andrographis paniculata[D]. Guangzhou: Guangzhou University of Chinese Medicine, 2012.
    [17]
    H SVEINSDÓTTIR, YAN F, ZHU Y, et al. Seed ageing-induced inhibition of germination and post-germination root growth is related to lower activity of plasma membrane H+-ATPase in maize roots[J]. Journal of Plant Physiology,2009,166(2):128−135. doi: 10.1016/j.jplph.2008.01.012
    [18]
    GUO L, YANG R, ZHOU Y, et al. Heat and hypoxia stresses enhance the accumulation of aliphatic glucosinolates and sulforaphane in broccoli sprouts[J]. European Food Research & Technology,2016,242(1):107−116.
    [19]
    GUO L P, YANG R, WANG Z, et al. Effect of NaCl stress on health-promoting compounds and antioxidant activity in the sprouts of three broccoli cultivars[J]. International Journal of Food Sciences & Nutrition,2014,65(4):476−481.
    [20]
    MADVAR A R, SARHADY S, MOHAMMADI M. Sulforaphane production and antioxidant enzyme activity in Lepidium draba seedlings in presence of Yeast extract and silver nitrate[J]. 2015.
    [21]
    WILLIAMS D J, CRITCHLEY C, PUN S, et al. Epithiospecifier protein activity in broccoli: The link between terminal alkenyl glucosinolates and sulphoraphane nitrile[J]. Phytochemistry,2008,69(16):2765−2773. doi: 10.1016/j.phytochem.2008.09.018
    [22]
    李江炜. 等离子体活化水对豆类种子萌发和生长的影响[D]. 厦门: 厦门大学, 2018

    LI J W. Effect of plasma activated water on the germination and growth of bean seeds[D]. Xiamen: Xiamen University, 2018.
    [23]
    邢新会, 张翀, 李和平, 等. 常压室温等离子体快速基因组突变技术和装置研究[C]// 全国生物化工技术发展研讨会. 中国化工学会, 2010.

    XING X H, ZHANG Z, LI H P, et al. Research on technology and device of rapid genome mutation in atmospheric and room temperature plasma[C]// National Biochemical Technology Development Seminar. China Chemical Industry Society, 2010.
    [24]
    ANDRASCH M, STACHOWIAK J, O SCHLÜTER, et al. Scale-up to pilot plant dimensions of plasma processed water generation for fresh-cut lettuce treatment[J]. Food Packaging and Shelf Life,2017:40−45.
    [25]
    赵曼如, 胡文忠, 于皎雪, 等. 茉莉酸甲酯对果蔬抗性, 抗氧化活性及品质影响的研究进展[J]. 食品工业科技,2020,41(4):328−332. [ZHAO M R, HU W Z, LI J X, et al. Research progress on the effect of methyl jasmonate on the resistance, antioxidant activity and quality of fruits and vegetables[J]. Science and Technology of Industry,2020,41(4):328−332.

    ZHAO M R, HU W Z, LI J X, et al. Research progress on the effect of methyl jasmonate on the resistance, antioxidant activity and quality of fruits and vegetables[J]. Science and Technology of Industry, 2020, 41(4): 328-332.
    [26]
    包金玉, 张聪聪, 马绍英, 等. 茉莉酸甲酯和水杨酸对西兰花毛状根增殖和萝卜硫素及信号分子含量的影响[J]. 植物生理学报,2020,56(6):1305−1312. [BAO J Y, ZHANG C C, MA S Y, et al. Effects of methyl jasmonate and salicylic acid on the proliferation of broccoli hairy roots and the content of sulforaphane and signal molecules[J]. Acta Plant Physiology,2020,56(6):1305−1312.

    BAO J Y, ZHANG C C, MA S Y, et al. Effects of methyl jasmonate and salicylic acid on the proliferation of broccoli hairy roots and the content of sulforaphane and signal molecules[J]. Acta Plant Physiology, 2020, 56(6): 1305-1312.
    [27]
    KIM H J, CHEN F, WANG X, et al. Effect of methyl jasmonate on phenolics, isothiocyanate, and metabolic enzymes in radish sprout (Raphanus sativus L.)[J]. J Agric Food Chem,2006,54(19):7263−7269. doi: 10.1021/jf060568c
    [28]
    VALGIMIGLI L, IORI R. Antioxidant and pro-oxidant capacities of ITCs[J]. Environmental & Molecular Mutagenesis,2010,50(3):222−237.
    [29]
    MITRA A, LI Y F, KLÄMPFL T G, et al. Inactivation of surface-borne microorganisms and increased germination of seed specimen by cold atmospheric plasma[J]. Food Bioprocess Tech.,2014,7(3):645−653. doi: 10.1007/s11947-013-1126-4
    [30]
    LINDSAY A, BYRNS B, KING W, et al. Fertilization of radishes, tomatoes, and marigolds using a large-volume atmospheric glow discharge[J]. Plasma Chemistry & Plasma Processing,2014,34(6):1271−1290.
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