Optimization of Formula of Bovine Colostrum Immunoglobulin Thermal Protective Agent by Response Surface Methodology

YANG Hong; LIU Aiguo; LIU Lizeng; QIANG Feng; YANG Yi

doi:10.13386/j.issn1002-0306.2021090302

Volume 43 Issue 12

Jun. 2022

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Science and Technology of Food Industry > 2022 > 43(12): 108-116. > DOI: 10.13386/j.issn1002-0306.2021090302

YANG Hong, LIU Aiguo, LIU Lizeng, et al. Optimization of Formula of Bovine Colostrum Immunoglobulin Thermal Protective Agent by Response Surface Methodology[J]. Science and Technology of Food Industry, 2022, 43(12): 108−116. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090302.

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Optimization of Formula of Bovine Colostrum Immunoglobulin Thermal Protective Agent by Response Surface Methodology

1.
Tianjin Key Laboratory of Food and Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
2.
Tianjin Tianshi College, Tianjin 301700, China
3.
Peterris (Tianjin) Trading Co., Ltd., Tianjin 300300, China

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Received Date: September 25, 2021
Available Online: April 13, 2022

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Abstract

Abstract

In order to screen an optimal thermal protective agent for immunoglobulin G in bovine colostrum, the IgG was extracted from bovine colostrum. Based on single factor experiments, the response value was the retention rate of IgG activity, and the response factors were glycine, maltitol and inulin. Box–Behnken experimental design on three levels and three variables was used for optimization of IgG thermal protectant. The structure of IgG was characterized by Fourier transform infrared Spectroscopy and endogenous fluorescence spectroscopy. The results showed that the best formula of IgG thermal protection agent was: Glycine 0.48%, inulin 14.98%, and maltitol 12.50%. Under these conditions, the IgG activity retention rate at 75 ℃ for 5 min was 36.59%, indicating that the response surface model had high accuracy and the optimized thermal protectant formula had practical application significance. The results of Fourier transform infrared spectrum and endogenous fluorescence spectrum analysis showed that compared with bovine colostrum IgG without compound heat protectant, the β-fold content decreased significantly (P<0.05), the irregular curl content decreased significantly (P<0.05), and the secondary conformation showed a more orderly state. The fluorescence intensity decreased, and IgG molecules piled up more tightly. The results showed that the structural stability of IgG in bovine colostrum was improved after adding compound heat protection agent. This research explored a new type of bovine colostrum IgG heat protectant formula, which would provide a reference for the further development and utilization of f functional foods from bovine colostrums.
- bovine colostrum,
- immunoglobulin G,
- thermal protective agent,
- formula optimization,
- infrared spectrum,
- endogenous fluorescence spectrum,
- structural change

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References

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