Inhibition Effect and Molecular Mechanism of Hypericin on Xanthine Oxidase

WANG Min; XU Guohui; ZHAO Yiling; WANG Gangqiang; HUANG Ganhui

doi:10.13386/j.issn1002-0306.2021090207

Volume 43 Issue 12

Jun. 2022

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Science and Technology of Food Industry > 2022 > 43(12): 92-99. > DOI: 10.13386/j.issn1002-0306.2021090207

WANG Min, XU Guohui, ZHAO Yiling, et al. Inhibition Effect and Molecular Mechanism of Hypericin on Xanthine Oxidase[J]. Science and Technology of Food Industry, 2022, 43(12): 92−99. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090207.

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Inhibition Effect and Molecular Mechanism of Hypericin on Xanthine Oxidase

1.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
2.
Jiangxi Fuzhou Inspection, Testing and Certification Center, Fuzhou 344000, China
3.
Sichuan Leshan Tianma Chenda Agricultural Co., Ltd., Leshan 614500, China

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Received Date: September 15, 2021
Available Online: April 11, 2022

Graphical Abstract

Abstract

Abstract

Dietary flavonoid has been reported to possess a high potential for inhibition xanthine oxidase (XOD). Herein, hypericin was investigated for its inhibition effect and interaction mechanism on XOD by enzyme inhibition kinetics, multi-spectroscopy and molecular docking. Enzyme kinetics analysis indicated that hypericin reversibly inhibited XOD in a mix-competitive manner with IC₅₀ value of (162.059±2.291) μmol/L and inhibition constant (Ki) value of (18.079±0.154) μmol/L. Multi-spectroscopy displayed that hypericin bound to XOD with high affinity, and the interaction was predominately driven by hydrogen bond and van der Waals forces. Circular dichroism (CD) demonstrated that hypericin induced the conformational change of XOD with increased α-helix and Random coil while reduced β-sheet and β-turn. Molecular docking suggested that hypericin interacted with residues located within the active pocket of XOD (e.g. GLN767, GLY797 and PHE798). These results would provide experimental and theoretical basis for hypericin in development of functional food or drug.
- hypericin,
- xanthine oxidase,
- enzyme kinetics,
- multi-spectroscopy,
- molecular docking

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References

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