Optimization of Extraction Process for Total Flavonoids from <i>Abelmoschus manihot</i> and Its Inhibitory Effect on Xanthine Oxidase

WANG Min; XU Guohui; ZHAO Yiling; WANG Gangqiang; ZHU Xiangyue; HUANG Ganhui

doi:10.13386/j.issn1002-0306.2021090191

Volume 43 Issue 12

Jun. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(12): 223-230. > DOI: 10.13386/j.issn1002-0306.2021090191

WANG Min, XU Guohui, ZHAO Yiling, et al. Optimization of Extraction Process for Total Flavonoids from Abelmoschus manihot and Its Inhibitory Effect on Xanthine Oxidase[J]. Science and Technology of Food Industry, 2022, 43(12): 223−230. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090191.

Citation:

PDF (3323 KB)

Optimization of Extraction Process for Total Flavonoids from Abelmoschus manihot and Its Inhibitory Effect on Xanthine Oxidase

1.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
2.
Jiangxi Fuzhou Inspection, Testing and Certification Center, Fuzhou 344000, China
3.
Sichuan Leshan Tianma Chenda Agricultural Co., Ltd., Leshan 614500, China

More Information

Received Date: September 14, 2021
Available Online: April 14, 2022

Graphical Abstract

Abstract

Abstract

Total flavonoids from Abelmoschus corolla were extracted by sodium dodecyl sulfate (SDS)-assisted ultrasonic wave, and its inhibition of xanthine oxidase (XOD) was studied, so as to achieve the efficient utilization of the resources of Abelmoschus manihot. Taking the content of total flavonoids as the index, the solid/liquid ratio, ultrasonic power and concentration of SDS were determined by single factor experiment. The extraction process of total flavonoids was optimized by combining Box-Behnken center and response surface analysis, and the effect of the extract on inhibiting XOD was determined. The results showed that the optimal extraction method was as follows: The solid/liquid ratio was 1:48 g/mL, the ultrasonic power was 320 W, the concentration of SDS was 0.05 g/mL. Under these conditions, the total flavonoids in Abelmoschus corolla were 4.63 mg/g. In the range of inhibitor concentration of 0.38~1.92 mg/mL, the inhibition rate of extracting solution was 98.35%, which had a good inhibitory effect on XOD.
- Abelmoschus manihot,
- total flavonoids,
- sodium dodecyl sulfate,
- extraction process,
- xanthine oxidase,
- inhibitory effect

FullText(HTML)

References (30)

References

[1]	李春洁. 黄蜀葵栽培技术[J]. 河北农业,2021(3):59−60. [LI Chunjie. Cultivation technology of Abelmoschus manihot[J]. Hebei Agriculture,2021(3):59−60. LI Chunjie. Cultivation technology of Abelmoschus manihot[J]. Hebei agriculture, 2021(3): 59-60.
[2]	张俊, 章方珺, 宋必卫. 黄蜀葵花总黄酮的质量标准及指纹图谱研究[J]. 海峡药学,2020,32(7):30−34. [ZHANG Jun, ZHANG Fangjun, SONG Biwei. Study on the quality standard and fingerprint of total flavonoids from Abelmoschus manihot (L.) medic flowers[J]. Channel of Pharmaceutical,2020,32(7):30−34. doi: 10.3969/j.issn.1006-3765.2020.07.011 ZHANG Jun, ZHANG Fangjun, SONG Biwei. Study on the quality standard and fingerprint of total flavonoids from Abelmoschus manihot (L. ) medic flowers[J]. Channel of Pharmaceutical, 2020, 32(07): 30-34. doi: 10.3969/j.issn.1006-3765.2020.07.011
[3]	董靖雯, 况泽安, 殷明晓, 等. 金丝桃苷通过下调PD-L1表达发挥抗非小细胞肺癌作用研究[J/OL]. 药学学报: 1-16[2021-09-10] DONG Jingwen, KUANG Zean, YIN Mingxiao, et al. Hyperoside exerts anti-tumor activity by reducing the PD-L1 level in non-small cell lung cancer[J/OL]. Acta Pharmaceutica Sinica, 1-16[2021-09-10].
[4]	郑传痴, 杨艳, 韦余, 等. 金丝桃苷对小鼠的抗疲劳作用及机制研究[J/OL]. 食品工业科技: 1−14[2021-09-10]. ZHANG Chuanchi, YANG Yan, WEI Yu, et al. Study on the effects and mechanism of hyperoside on anti-fatigue in mice[J]. Science and Technology of Food Industry, 1−14[2021-09-10].
[5]	李婧, 刘玉翠, 高宇丹, 等. 黄蜀葵和秋葵不同部位黄酮类成分含量测定方法研究[J]. 江西中医药,2020,51(7):68−70. [LI Jin, LIU Yucui, GAO Yudan, et al. Study on the determination method of flavonoids in different parts of Abelmoschus manihot and okra[J]. Jiangxi Journal of Traditional Chinese Medicine,2020,51(7):68−70. LI Jin, LIU Yucui, GAO Yudan, et al. Study on the determination method of flavonoids in different parts of Abelmoschus manihot and okra[J]. Jiangxi Journal of Traditional Chinese Medicine, 2020, 51(7): 68-70.
[6]	王海东, 谢建祥, 林伟青. Eupatilin对黄嘌呤氧化酶活性抑制的实验研究[J]. 实用中西医结合临床,2015,15(9):87−89. [WANG Haidong, XIE Jianxiang, LIN Weiqing. Study on inhibitory effect of eupatilin on xanthine oxidase[J]. Clinical Practice of Integrated Traditional Chinese and Western Medicine,2015,15(9):87−89. WANG Haidong, XIE Jianxiang, LIN Weiqing. Study on inhibitory effect of eupatilin on xanthine oxidase[J]. Clinical practice of integrated Traditional Chinese and Western medicine, 2015, 15(9): 87-89.
[7]	WANG Yajie, ZHANG Guowen, PAN Junhui, et al. Novel insights into the inhibitory mechanism of kaempferol on xanthine oxidase[J]. Journal of Agricultural and Food Chemistry,2015,63:526−534. doi: 10.1021/jf505584m
[8]	YAN Jiakai, ZHANG Guowen, HU Yuting, et al. Effect of luteolin on xanthine oxidase: Inhibition kinetics and interaction mechanism merging with docking simulation[J]. Food Chemistry,2013,141:2766−2773.
[9]	王鹏, 王玉, 梅菊, 等. 黄蜀葵花中金丝桃苷的提取工艺研究[J]. 食品与发酵科技,2021,57(3):76−80,89. [WANG Peng, WANG Yu, MEI Ju, et al. Study on extraction technology of hyperin from Abelmoschus manihot (L)[J]. Food and Fermentation Sciences & Technology,2021,57(3):76−80,89. WANG Peng, WANG Yu, MEI Ju, et al. Study on extraction technology of hyperin from Abelmoschus manihot (L)[J]. Food and Fermentation Sciences & Technology, 2021, 57(3): 76-80, 89.
[10]	宋健刚, 钟方丽, 王晓林, 等. 提取黑果腺肋花楸果中金丝桃苷的工艺[J]. 食品工业,2019,40(7):113−119. [SONG Jiangang, ZHONG Fangli, WANG Xiaolin, et al. Extraction of hyperin from Aronia melanocarpa fruit by lonic liquid ultrasonic assisted extraction[J]. Food Industry,2019,40(7):113−119. SONG Jiangang, ZHONG Fangli, WANG Xiaolin, et al. Extraction of hyperin from Aronia melanocarpa fruit by lonic liquid ultrasonic assisted extraction[J]. Food Industry, 2019, 40(7): 113-119.
[11]	刘可福. 银杏叶有效成分超临界提取技术[J]. 海峡药学,2021,33(3):49−52. [LIU Kefu. Supercritical extraction technology of effective components from Ginkgo biloba leaves[J]. Channel of Pharmaceutical,2021,33(3):49−52. doi: 10.3969/j.issn.1006-3765.2021.03.014 LIU Kefu. Supercritical extraction technology of effective components from Ginkgo biloba leaves[J]. Channel of Pharmaceutical, 2021, 33(3): 49-52. doi: 10.3969/j.issn.1006-3765.2021.03.014
[12]	彭洁, 吴菲菲, 李化强, 等. 超声辅助酸性乙醇提取紫甘薯花青素的响应面法优化工艺[J]. 食品安全质量检测学报,2021,12(7):2773−2781. [PENG Jie, WU Feifei, LI Huaqiang, et al. Optimization of the ultrasonic-assisted acid ethanol extraction process of anthocyanin from Ipomoea batatas (L.) lam. using response surface methodology[J]. Journal of Food Safety and Quality,2021,12(7):2773−2781. PENG Jie, WU Feifei, LI Huaqiang, et al. Optimization of the ultrasonic-assisted acid ethanol extraction process of anthocyanin from Ipomoea batatas (L. ) lam. using response surface methodology[J]. Journal of Food Safety and Quality, 2021, 12(7): 2773-2781.
[13]	丁梁斌, 马春梅, 赵苹苹, 等. 响应面法优化酶解-超声辅助提取鸡血藤多糖工艺研究[J]. 中国食品添加剂,2021,32(7):88−96. [DING Liangbin, MA Chunmei, ZHAO Pingping, et al. Optimization of enzymatic hydrolysis-ultrasonic assisted extraction of polysaccharides from spatholobi by response surface methodology[J]. China Food Additives,2021,32(7):88−96. DING Liangbin, MA Chunmei, ZHAO Pingping, et al. Optimization of enzymatic hydrolysis-ultrasonic assisted extraction of polysaccharides from spatholobi by response surface methodology[J]. China Food Additives, 2021, 32(7): 88-96.
[14]	王敏, 田珍燕, 王蔚新. 板栗壳多酚的提取及其抗氧化性能的研究[J]. 食品研究与开发,2018,39(22):66−72. [WANG Min, TIAN Zhenyan, WANG Weixin. Study on the extraction and antioxidant properties of polyphenols from chestnut shell[J]. Food Research and Development,2018,39(22):66−72. doi: 10.3969/j.issn.1005-6521.2018.22.012 WANG Min, TIAN Zhenyan, WANG Weixin. Study on the extraction and antioxidant properties of polyphenols from chestnut shell[J]. Food Research and Development, 2018, 39(22): 66-72. doi: 10.3969/j.issn.1005-6521.2018.22.012
[15]	陈铁壁, 赵昌会, 何福林, 等. 超声波—表面活性剂协同辅助提取江永香柚籽多糖工艺的优化[J]. 食品与机械,2016,32(7):161−165. [CHEN Tiebi, ZHAO Huichang, HE Fulin, et al. Optimization on extraction of polysaccharides from seeds of Citrus grandis using surfactant assisted ultrasonic extraction[J]. Food & Machinery,2016,32(7):161−165. CHEN Tiebi, ZHAO Huichang, HE Fulin, et al. Optimization on extraction of polysaccharides from seeds of Citrus grandis using surfactant assisted ultrasonic extraction[J]. Food & Machinery, 2016, 32(7): 161-165.
[16]	朱欣, 郭红英, 谭兴和, 等. 超声波-阴/阳离子表面活性剂协同提取茅岩莓总黄酮的工艺研究[J]. 食品工业科技,2016,37(1):213−218. [ZHU Xin, GUO Hongying, TAN Xinghe, et al. Ultrasonic-assisted anionic and cationic surfactant systems extraction of total flavonoids from Ampelopsis grossedentata[J]. Science and Technology of Food Industry,2016,37(1):213−218. ZHU Xin, GUO Hongying, TAN Xinghe, et al. Ultrasonic-assisted anionic and cationic surfactant systems extraction of total flavonoids from Ampelopsis grossedentata[J]. Science and Technology of Food Industry, 2016, 37(1): 213-218.
[17]	李娇, 李江, 付鹏, 等. 十二烷基硫酸钠强化柴胡皂苷的提取工艺[J]. 昆明医科大学学报,2020,41(6):15−22. [LI Jiao, LI Jiang, FU Peng, et al. Study on the process of enhanced of Saikosaponin by surfactant[J]. Journal of Kunming Medical University,2020,41(6):15−22. doi: 10.3969/j.issn.1003-4706.2020.06.004 LI Jiao, LI Jiang, FU Peng, et al. Study on the process of enhanced of Saikosaponin by surfactant[J]. Journal of Kunming Medical University, 2020, 41(6): 15-22. doi: 10.3969/j.issn.1003-4706.2020.06.004
[18]	张馨心, 宫福帅, 欧小女, 等. 表面活性剂强化超声波提取龙牙楤木齐墩果酸的研究[J]. 人参研究,2021,33(2):44−46. [ZHANG Xinxin, GONG Fushuai, OU Xiaonv, et al. Study on oleanolic acid abstracted from Aralia elata of surfactant and ultrasonic[J]. Ginseng Research,2021,33(2):44−46. ZHANG Xinxin, GONG Fushuai, OU Xiaonv, et al. Study on oleanolic acid abstracted from Aralia elata of surfactant and ultrasonic[J]. Ginseng Research, 2021, 33(2): 44-46.
[19]	吴睿, 穆俐俐, 张丹, 等. 超声波辅助提取薯皮中去氢表雄酮的工艺研究[J]. 化学研究与应用,2021,33(8):1577−1581. [WU Rui, MU Lili, ZHANG Dang, et al. Technical study for ultrasonic-assisted extraction of dehydroepiandrosterone from sweet potatoes skin[J]. Chemical Research and Application,2021,33(8):1577−1581. doi: 10.3969/j.issn.1004-1656.2021.08.021 WU Rui, MU Lili, ZHANG Dang, et al. Technical study for ultrasonic-assisted extraction of dehydroepiandrosterone from sweet potatoes skin[J]. Chemical Research and Application, 2021, 33(8): 1577-1581. doi: 10.3969/j.issn.1004-1656.2021.08.021
[20]	王志伟, 兰圆圆, 李琦玲, 等. 响应面法优化超声辅助提取三七花总黄酮工艺研究[J]. 广东化工,2021,48(3):27−29. [WANG Zhiwei, LAN Yuanyuan, LI Qiling, et al. Optimization of extraction process for total flavonoid with ultrasound assisted from Panax notoginseng flowers by response surface methodology[J]. Guangdong Chemical Industry,2021,48(3):27−29. doi: 10.3969/j.issn.1007-1865.2021.03.013 WANG Zhiwei, LAN Yuanyuan, LI Qiling, et al. Optimization of extraction process for total flavonoid with ultrasound assisted from Panax notoginseng flowers by response surface methodology[J]. Guangdong Chemical Industry, 2021, 48(3): 27-29. doi: 10.3969/j.issn.1007-1865.2021.03.013
[21]	刘宏, 夏慧敏, 李娜, 等. 响应面法优化荞麦芽黄酮的提取工艺及其抗氧化性和稳定性的研究[J]. 世界科学技术-中医药现代化,2020,22(8):2870−2882. [LIU Hong, XIA Huimin, LI Na, et al. Optimization of extraction process and antioxidant and stability of flavonoids from Fagopyrum esculentum Moench by response surface methodology[J]. Modernization of Traditional Chinese Medicine and Materia Materia-World Science and Technology,2020,22(8):2870−2882. LIU Hong, XIA Huimin, LI Na, et al. Optimization of extraction process and antioxidant and stability of flavonoids from Fagopyrum esculentum Moench by response surface methodology[J]. Modernization of Traditional Chinese Medicine and Materia Materia-World Science and Technology, 2020, 22(8): 2870-2882.
[22]	陈海龙, 江帆, 丁小艳, 等. 响应面分析法优化黄蜀葵花中金丝桃苷、异槲皮苷、槲皮素提取工艺的研究[J]. 食品工业科技,2015,36(24):216−221. [CHEN Hailong, JIANG Fan, DING Xiaoyan, et al. Optimization of extraction technology for hyperoside, isoquercitrin, quercetin in Abelmoschus manihot (L.) medic. by response surface method[J]. Science and Technology of Food Industry,2015,36(24):216−221. CHEN Hailong, JIANG Fan, DING Xiaoyan, et al. Optimization of extraction technology for hyperoside, isoquercitrin, quercetin in Abelmoschus manihot (L. ) medic. by response surface method[J]. Science and Technology of Food Industry, 2015, 36(24): 216-221.
[23]	ANDRIJA C, BOR K, DAVID H, et al. Response surface methodology and artificial neural network approach for the optimization of ultrasound-assisted extraction of polyphenols from garlic[J]. Food and Chemical Toxicology,2020,135:110976. doi: 10.1016/j.fct.2019.110976
[24]	HARTINI, MEGA RENOVASI TARA, KUN HARISMAH, et al. Optimization of batch extraction of functional ingredients from stevia leaves with response surface methodology[J]. Journal of Physics: Conference Series, 2021. 1858(1).
[25]	MALIHE S K, SOMAYEH F. Evaluation of the extraction process of Arenaria hispanica L. using response surface methodology on amounts of total phenolic content, total flavonoid content and the antioxidant activity[J]. Advances in Traditional Medicine, 2021.
[26]	李艳艳, 王倩倩, 苏圆圆, 等. 响应面法优化提取曼地亚红豆杉总黄酮及抗氧化活性[J]. 北方园艺,2021(5):94−102. [LI Yanyan, WANG Qianqian, SU Yuanyuan, et al. Optimization of extraction and antioxidant activity of total flavonoids from Taxus media by response surface methodology[J]. Northern Horticulture,2021(5):94−102. LI Yanyan, WANG Qianqian, SU Yuanyuan, et al. Optimization of extraction and antioxidant activity of total flavonoids from Taxus media by response surface methodology[J]. Northern Horticulture, 2021(5): 94-102.
[27]	杨海涛, 曹小燕. 酶-超声辅助提取苦荞秆中总黄酮及抗氧化活性研究[J]. 中国酿造,2016,35(9):72−76. [YANG Haitao, CAO Xiaoyan. Enzymatic-ultrasonic assisted extraction of total flavonoids from Fagopyrum tataricum straw and its antioxidant activity[J]. China Brewing,2016,35(9):72−76. doi: 10.11882/j.issn.0254-5071.2016.09.017 YANG Haitao, CAO Xiaoyan. Enzymatic-ultrasonic assisted extraction of total flavonoids from Fagopyrum tataricum straw and its antioxidant activity[J]. China Brewing, 2016, 35(9): 72-76. doi: 10.11882/j.issn.0254-5071.2016.09.017
[28]	朱德艳, 陈晗. 超声波辅助提取葛根中总黄酮及其体外抗氧化性的研究[J]. 食品研究与开发,2017,38(2):62−65. [ZHU Deyan, CHEN Han. Ultrasonic-assisted extraction of total flavonoids from Pueraria radix and its antioxidantion[J]. Food Research and Development,2017,38(2):62−65. doi: 10.3969/j.issn.1005-6521.2017.02.013 ZHU Deyan, CHEN Han. Ultrasonic-assisted Extraction of Total Flavonoids from Pueraria radix and Its Antioxidantion[J]. Food Research and Development, 2017, 38(2): 62-65. doi: 10.3969/j.issn.1005-6521.2017.02.013
[29]	T J LIN. 过量表面活性剂在乳化中的反作用[J]. 日用化学品科学,1986(1):35−40. [T J LIN. Reaction of excess surfactant in emulsification[J]. Daily Chemical Science,1986(1):35−40. T. J. LIN. Reaction of excess surfactant in emulsification[J]. Daily Chemical Science, 1986(1): 35-40.
[30]	王冉, 李健, 黎晨晨, 等. 响应面法优化东北红松针总黄酮的超声辅助乙醇提取工艺[J]. 食品工业科技,2018,39(4):143−149. [WANG Ran, LI Jian, LI Chenchen, et al. Optimization of ultrasonic-assisted ethanol extraction of total flavonoids from Pinus koraiensis needles by response surface methodology[J]. Science and Technology of Food Industry,2018,39(4):143−149. WANG Ran, LI Jian, LI Chenchen, et al. Optimization of ultrasonic-assisted ethanol extraction of total flavonoids from Pinus koraiensis needles by response surface methodology[J]. Science and Technology of Food Industry, 2018, 39(4): 143-149.

Supplements (0)

Cited By

Cited by

Periodical cited type(12)

1.	林柳雁，王丽，萧绮莉，蒋小红，陈惠冰. 成人炎症性肠病患者经口营养管理的证据总结. 护理实践与研究. 2025(02): 244-251 .
2.	雷露，吕沛然，张宁，梁越，赵耀，赵娴. 中医治疗炎症性肠病机制的研究进展. 中医药学报. 2024(02): 116-121 .
3.	李振，崔艳红，刘长忠，袁梦. 枯草芽孢杆菌对环境耐受性和产中性蛋白酶能力研究. 黑龙江畜牧兽医. 2024(04): 81-87+92 .
4.	耿英杰，戚浩龙，李园，蔡晓青. 益生菌治疗炎症性肠病的研究进展. 中国医药工业杂志. 2024(08): 1069-1077 .
5.	李文，刘夫锋. 基于益生菌口服药物递送系统的研究进展. 微生物学报. 2024(11): 4086-4105 .
6.	曾馨苑，吴严玮. 益生菌联合复方谷氨酰胺和马来酸曲美布汀治疗腹泻型肠易激综合征的疗效观察. 中国处方药. 2024(11): 105-107 .
7.	乌云，浩斯，巴达玛斯仁，锡林其其格，斯琴. 蒙医大肠宝如病与炎症性肠病的相关研究进展. 中国民族医药杂志. 2023(01): 65-67 .
8.	王秋珍，邓自腾，兰静，刘雪连，范祥伟，薛毅，马云飞. 干酪乳杆菌对DSS诱导结肠炎小鼠焦虑样行为的影响及对结肠的保护作用. 中国农业大学学报. 2023(04): 139-146 .
9.	杨硕，唐宗馨，段勃帆，陈禹含，郭欢新，孟祥晨. 双歧杆菌及其制剂对炎症性肠病作用机制研究进展. 食品科学. 2023(05): 275-281 .
10.	侯保秋，董怡文，刘方. 一株分离自牦牛乳中的副干酪乳杆菌ZZ102对溃疡性结肠炎的缓解作用研究. 中国乳品工业. 2023(10): 21-25 .
11.	刘柘君，刘振权，孙文燕. 复方益生菌粉提高小鼠免疫力的实验研究. 食品与药品. 2023(05): 456-461 .
12.	杨敏琪，张吉翔，董卫国. 益生菌治疗炎性肠病的研究进展. 疑难病杂志. 2022(11): 1202-1205 .