Optimization of Extraction Technology of Total Flavonoids from <i>Passiflora edulis</i> peel by Ultrasonic Assisted with Complex Enzyme and Its Antioxidant Activity

ZHANG Xiaomei; WANG Cong; JIN Xiaolin; HOU Yini; ZHENG Liping

doi:10.13386/j.issn1002-0306.2021090175

Volume 43 Issue 12

Jun. 2022

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Science and Technology of Food Industry > 2022 > 43(12): 215-222. > DOI: 10.13386/j.issn1002-0306.2021090175

ZHANG Xiaomei, WANG Cong, JIN Xiaolin, et al. Optimization of Extraction Technology of Total Flavonoids from Passiflora edulis peel by Ultrasonic Assisted with Complex Enzyme and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(12): 215−222. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090175.

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Optimization of Extraction Technology of Total Flavonoids from Passiflora edulis peel by Ultrasonic Assisted with Complex Enzyme and Its Antioxidant Activity

1.
School of Chemical and Food Engineering, Zhengzhou Institute of Technology, Zhengzhou 450053, China
2.
Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China

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Received Date: September 13, 2021
Available Online: April 14, 2022

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Abstract

Abstract

The total flavonoids form Passiflora edulis peel were extracted by compound enzyme assisted ultrasonic method. Taking the yield of flavonoids as the index, the effects of the amount of compound enzyme, enzymatic hydrolysis time, liquid-solid ratio and ultrasonic time on the extraction of total flavonoids from Passiflora edulis peel were analyzed by single factor tests. The main influencing factors and optimal combination of flavonoids extraction were further analyzed by response surface test (Box-Behnken), and the scavenging rate and reducing power of DPPH free radical and hydroxyl free radical were detected in vitro. The results showed that the optimum extraction conditions for ultrasonic extraction of total flavonoids from Passiflora edulis peel assisted by composite enzyme were as follows: The ratio of cellulase to pectinase was 2:1, the dosage of composite enzyme was 4.8%, the enzymolysis time was 1 h, the ethanol concentration was 60%, the liquid-material radio was 30:1 mL/g, and the ultrasonic time was 41 min. Under the optimized conditions, the total flavonoids yield was (2.20%±0.05%), which was similar to the predicted yield 2.24% (<1%) of the regression model. The results of antioxidant activity showed that the scavenging rates of DPPH radical and hydroxyl radical were 90.8% and 84.1% when the concentration of flavonoids from Passiflora edulis peel reached 0.44 mg/mL and 44 µg/mL respectively. The extraction of total flavonoids from Passiflora edulis peel by complex enzyme assisted ultrasound could provide a way for the resource utilization of thyme peel. Based on the above research results, the total flavonoids from Passiflora edulis peel had good antioxidant activity and were an ideal natural antioxidant.
- Passiflora edulis peel,
- flavonoids,
- complex enzyme and ultrasound-assisted method,
- antioxidant activity

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References

[1]	成文韬, 袁启凤, 肖图舰, 等. 西番莲果实生物活性成分及生理功能研究进展[J]. 食品工业科技, 2018, 39(16): 346-351 CHENG W T, YUAN Q F, XIAO T J, et al. Research progress on bioactive substances and physiological functions of passion fruit[J]. Science and Technology of Food Industry, 2018, 39(16): 346-351.
[2]	COSTA C F, FUSIEGER A, ANDRETTA M, et al. Short communication: Potential use of passion fruit (Passiflora cincinnata) as a biopreservative in the production of coalho cheese, a traditional Brazilian cheese[J]. Journal of Dairy Science,2020,103(4):3082−3087. doi: 10.3168/jds.2019-17791
[3]	REIS L D, FACCO E, SALVADOR M, et al. Antioxidant potential and physicochemical characterization of yellow, purple and orange passion fruit[J]. Journal of Food Science & Technology,2018,55(3):2679−2691.
[4]	乔沛, 殷菲胧, 李静, 等. 百香果采后贮藏保鲜研究进展[J]. 保鲜与加工,2020,20(4):220−225. [QIAO P, YIN F L, LI J, et al. Advances on postharvest storage and preservation of passion fruit[J]. Storage and Process,2020,20(4):220−225. doi: 10.3969/j.issn.1009-6221.2020.04.035 QIAO P, YIN F L, LI J, et al. Advances on postharvest storage and preservation of passion fruit[J]. Storage and Process, 2020, 20(4): 220-225. doi: 10.3969/j.issn.1009-6221.2020.04.035
[5]	ZHANG W, NIU X L, YANG J Y. First report of postharvest fruit rot on passion fruit (Passiflora edulis) caused by Lasiodiplodia theobromae in Mainland China[J]. Plant Disease, 2020, 105(4).
[6]	MOURA F, MACAGNAN F T, KLEIN B, et al. Metabolic properties of partially hydrolyzed pectin from passion fruit peel[J]. Bioactive Carbohydrates and Dietary Fibre,2021,25(4):100256.
[7]	GUO R, TIAN S, LI X, et al. Pectic polysaccharides from purple passion fruit peel: A comprehensive study in macromolecular and conformational characterizations[J]. Carbohydrate Polymers,2019,229:115406.
[8]	许梦圆, 景继月, 朱璟君, 等. 食用西番莲中酚类物质结构与生物活性研究进展[J]. 天然产物研究与开发,2021,33(7):1251−1265. [XU M Y, JING J Y, ZHU J J, et al. Research progress on structures and bioactivities of the phenolic compounds in edible Passiflora linn J]. Natural Product Research and Development,2021,33(7):1251−1265.
[9]	FAVERI A D, FAVERI R D, BROERING M F, et al. Effects of passion fruit peel flour (Passiflora edulis f. flavicarpa O. Deg.) in cafeteria diet-induced metabolic disorders[J]. Journal of Ethnopharmacology,2019,250:112482.
[10]	周葵. 西番莲综合加工关键技术研究[D]. 南宁: 广西大学, 2015 ZHOU K. Key technology research in integrated processing of passion fruit[D]. Nanning: Guangxi University, 2015.
[11]	史斌斌, 袁启凤, 李仕品. 西番莲营养及功能性成分的研究进展[J]. 贵州农业科学,2019,47(12):95−98. [SHI B B, YUAN Q F, LI S P. Research progress on nutrition and functional components of Passiflora coerulea[J]. Guizhou Agricultural Sciences,2019,47(12):95−98. doi: 10.3969/j.issn.1001-3601.2019.12.021 SHI B B, YUAN Q F, LI S P. Research progress on nutrition and functional components of Passiflora coerulea[J]. Guizhou Agricultural Sciences, 2019, 47(12): 95-98. doi: 10.3969/j.issn.1001-3601.2019.12.021
[12]	DOMINGUEZ-RODRIGUEZ G, GARCIA M C, PLAZA M, et al. Revalorization of Passiflora species peels as a sustainable source of antioxidant phenolic compounds[J]. Science of the Total Environment,2019,696:134030. doi: 10.1016/j.scitotenv.2019.134030
[13]	DKRH A, NJW B, APD B, et al. Garlic passion fruit (Passiflora tenuifila Killip): Assessment of eventual acute toxicity, anxiolytic, sedative, and anticonvulsant effects using in vivo assays[J]. Food Research International,2020,128:108813. doi: 10.1016/j.foodres.2019.108813
[14]	罗文珊, 吴佩佩, 谭强, 等. 百香果深加工技术研究进展[J]. 农产品加工,2018(4):69−74. [LUO WS, WU P P, TAN Q, et al. Research progress on deep processing technology of passion fruit[J]. Farm products Processing,2018(4):69−74. LUO WS, WU P P, TAN Q, et al. Research progress on deep processing technology of passion fruit[J]. Farm products Processing, 2018(4): 69-74.
[15]	戴钰洁. 百香果深加工工艺研究进展[J]. 漳州职业技术学院学报,2020,22(4):93−98. [DAI Y J. Progress in research on the deep processing technology for passion fruit[J]. Journal of Zhangzhou Institute of Technology,2020,22(4):93−98. DAI Y J. Progress in research on the deep processing technology for passion fruit[J]. Journal of Zhangzhou Institute of Technology, 2020, 22 (4): 93-98.
[16]	李欣燃, 张兴, 翁贵英, 等. 百香果黄酮提取工艺优化及其抗氧化活性研究[J]. 食品工业科技,2020,41(24):106−112. [LI X R, ZHANG X, WENG G Y, et al. Optimization of extraction process of total flavonoids from passion fruit and its antioxidant activity[J]. Science and Technology of Food Industry,2020,41(24):106−112. LI X R, ZHANG X, WENG G Y, et, al. Optimization of extraction process of total flavonoids from passion fruit and its antioxidant activity[J]. Science and Technology of Food Industry, 2020, 41(24): 106-112.
[17]	廖兰, 吴俊良. 逆流法提取百香果果皮中总黄酮的工艺研究[J]. 广西科技大学学报,2018,29(2):91−96. [LIAO L, WU J L. Study on countercurrent extraction of total flavonoids from Passiflora edulis peel[J]. Journal of Guangxi University of Science and Technology,2018,29(2):91−96. LIAO L, WU J L. Study on countercurrent extraction of total flavonoids from Passiflora edulis peel[J]. Journal of Guangxi University of Science and Technology, 2018, 29(2): 91-96.
[18]	余启明, 陈昌盛, 廖娟, 等. 百香果果皮的微波预处理-超声波提取工艺及其抗氧化活性研究[J]. 化工技术与开发,2019,48(11):18−21. [YU Q M, CHEN C S, LIAO J, et al. Microwave pretreatment-ultrasonic extraction technology of Passiflora edulis sims and its antioxidant activity[J]. Technology & Development of Chemical Industry,2019,48(11):18−21. doi: 10.3969/j.issn.1671-9905.2019.11.005 YU Q M, CHEN C S, LIAO J, et al. Microwave pretreatment-ultrasonic extraction technology of passiflora edulis sims and its antioxidant activity[J]. Technology & Development of Chemical Industry, 2019, 48(11): 18-21. doi: 10.3969/j.issn.1671-9905.2019.11.005
[19]	徐玉霞, 王华斌. 酶法提取海红果总黄酮工艺及海红果黄酮粗提物对HeLa细胞的增殖作用[J]. 中国农业大学学报,2013,18(1):119−127. [XU Y X, WANG H B. Effects of extracting technology of total flavonoids in malus micromalus Makino by enzymic treatment and its flavonoid crude extract on proliferation of hela cells in vitro[J]. Journal of China Agricultural University,2013,18(1):119−127. XU Y X, WANG H B. Effects of extracting technology of total flavonoids in malus micromalus Makino by enzymic treatment and its flavonoid crude extract on proliferation of hela cells in vitro[J]. Journal of China Agricultural University, 2013, 18(01): 119-127.
[20]	张丽静, 付劢, 张文会, 等. 复合酶辅助超声提取西藏芜菁总黄酮工艺优化及抗氧化活性分析[J]. 食品工业科技,2021,42(6):174−180. [ZHANG L J, FU M, ZHANG W H, et al. Enzyme-assisted ultrasonic extraction of total flavonoids from Brassica rapa L. optimization and its antioxidant activity analysis[J]. Science and Technology of Food Industry,2021,42(6):174−180. ZHANG L J, FU M, ZHANG W H, et, al. Enzyme-assisted ultrasonic extraction of total flavonoids from Brassica rapa L. optimization and its antioxidant activity analysis[J]. Science and Technology of Food Industry, 2021, 42(6): 174-180.
[21]	王洪彪. 乌拉草黄酮化学成分分析及其抗氧化和抑菌活性研究[D]. 吉林: 吉林化工学院, 2020 WANG H B. Study on the chemical constituents and antioxidant and bacteriostatic activities of flavonoids from Carex meyeriana kunth[D]. Jilin: Jilin Institute of Chemical Technology, 2020.
[22]	张会香, 杨世军, 蓝华生, 等. 超声波辅助法提取百香果壳中黄酮的工艺及其抗氧化性研究[J]. 食品研究与开发,2020,41(1):123−129. [ZHANG H X, YANG S J, LAN H S, et al. Study on extraction and antioxidant activity of flavonoids from shells of passion fruit with ultrasonic-assisted method[J]. Food Research and Development,2020,41(1):123−129. ZHANG H X, YANG S J, LAN H S, et al. Study on extraction and antioxidant activity of flavonoids from shells of passion fruit with ultrasonic-assisted method[J]. Food Research and Development, 2020, 41(1): 123-129.
[23]	杨宗玲, 李晗, 范方宇, 等. 超声辅助酶法提取无籽刺梨果渣中黄酮的工艺优化及其抗氧化活性[J]. 食品工业科技,2021,42(13):184−192. [YANG Z L, LI H, FAN F Y, et al. Ultrasound-assisted enzymatic extraction of flavonoids from Rosa sterilis prmace and its antioxidant activity[J]. Science and Technology of Food Industry,2021,42(13):184−192. YANG Z L, LI H, FAN F Y, et, al. Ultrasound-assisted enzymatic extraction of flavonoids from Rosa sterilis prmace and its antioxidant activity[J]. Science and Technology of Food Industry, 2021, 42(13): 184-192.
[24]	张倩茹. 玉米芯多糖的菌酶协同发酵工艺及其体外活性研究[D]. 呼和浩特: 内蒙古农业大学, 2018 ZHANG Q R. Fermentation of corncob by microorganism and enzyme to produce polysaccharides and its in vitro activity[D]. Huhhot: Inner Mongolia Agricultural University, 2018.
[25]	王家皓, 贲蕾洁, 符茜, 等. 南苜蓿叶总黄酮提取及抑菌抗氧化活性[J]. 江苏农业科学,2020,48(17):201−205,214. [WANG J H, BI L J, FU Q, et al. Extraction of total flavonoids from leaves of Medicage hispida and its antibacterial and antioxidant activities[J]. Jiangsu Agricultural Sciences,2020,48(17):201−205,214. WANG J H, BI L J, FU Q, et al. Extraction of total flavonoids from leaves of Medicage hispida and its antibacterial and antioxidant activities[J]. Jiangsu Agricultural Sciences, 2020, 48(17): 201-205, 214.
[26]	史建俊, 李伟伟, 王免, 等. 超声波协同酶法提取黄山贡菊总黄酮及其抗氧化活性[J]. 安徽农业科学,2020,48(24):174−177. [SHI J J, LI W W, WANG M, et al. Ultrasonic assisted enzymatic extraction of total flavonoids from Huangshan Gongju and antioxidant activity[J]. Journal of Anhui Agricultural Sciences,2020,48(24):174−177. doi: 10.3969/j.issn.0517-6611.2020.24.049 SHI J J, LI W W, WANG M, et al. Ultrasonic assisted enzymatic extraction of total flavonoids from Huangshan Gongju and antioxidant activity[J]. Journal of Anhui Agricultural Sciences, 2020, 48(24): 174-177. doi: 10.3969/j.issn.0517-6611.2020.24.049
[27]	刘媛洁, 张良. 响应面法优化复合酶辅助超声波提取柚子皮总黄酮工艺[J]. 食品工业科技,2019,40(23):143−150. [LIU Y J, ZHANG L. Optimization of enzymatic assisted ultrasonic extraction of total flavonoids from grapefruit peel by response surface methodology[J]. Science and Technology of Food Industry,2019,40(23):143−150. LIU Y J, ZHANG L. Optimization of enzymatic assisted ultrasonic extraction of total flavonoids from grapefruit peel by response surface methodology[J]. Science and Technology of Food Industry, 2019, 40(23): 143-150.
[28]	童汉清, 海金萍, 李树长, 等. 响应面法优化超声辅助溶剂提取香蕉5-羟色胺的工艺[J]. 食品研究与开发,2020,41(20):124−130. [TONG H Q, HAI J P, LI S C, et al. Optimization of ultrasound-assisted solvent extraction of 5-hydroxytryptamine from banana by response surface methodology[J]. Food Research and Development,2020,41(20):124−130. doi: 10.12161/j.issn.1005-6521.2020.20.021 TONG H Q, HAI J P, LI S C, et al. Optimization of ultrasound-assisted solvent extraction of 5-Hydroxytryptamine from banana by response surface methodology[J]. Food Research and Development, 2020, 41(20): 124-130. doi: 10.12161/j.issn.1005-6521.2020.20.021

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