Optimization of Enzymatic Hydrolysis Process of Immune Active Peptide in Chub Mackerel

LIAO Huiqi; CAO Shaoqian; YANG Hua; QI Xiangyang

doi:10.13386/j.issn1002-0306.2021090087

Volume 43 Issue 14

Jul. 2022

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Science and Technology of Food Industry > 2022 > 43(14): 163-170. > DOI: 10.13386/j.issn1002-0306.2021090087

LIAO Huiqi, CAO Shaoqian, YANG Hua, et al. Optimization of Enzymatic Hydrolysis Process of Immune Active Peptide in Chub Mackerel[J]. Science and Technology of Food Industry, 2022, 43(14): 163−170. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090087.

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Optimization of Enzymatic Hydrolysis Process of Immune Active Peptide in Chub Mackerel

Zhejiang Wanli University, Ningbo 315100, China

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Received Date: September 06, 2021
Available Online: May 10, 2022

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Abstract

In order to optimize the enzymatic extraction process of chub mackerel immune active peptide, taking the proliferation rate and degree of hydrolysis of mouse spleen lymphocytes as indicators, on the basis of single factor tests, the enzyme addition amount, enzymatic hydrolysis time and enzymatic hydrolysis temperature were selected as the investigation factors, and the Box-Behnken method was used for three factors and three levels response surface test design to determine the best enzymatic preparation process of chub mackerel immune active peptide. The amino acid composition of the obtained immune active peptide of chub mackerel was analyzed. The results showed that neutral protease was the best protease, and the best enzymatic hydrolysis conditions were enzyme addition 8800 U/g, time 7 h and temperature 52 ℃. The verification experiment found that the relative proliferation rate of mouse spleen lymphocytes obtained under these conditions was 48.11%±2.67%, which was close to the predicted value of regression equation. At the same time, chub mackerel immune active peptide was rich in amino acids, including 45.52% essential amino acids and 32.00% hydrophobic amino acids. The results would provide a theoretical basis for enzymatic preparation of immune active peptide for chub mackerel, and a reference for further realization of high value utilization of chub mackerel resources.
- chub mackerel,
- immune active peptide,
- enzymolysis,
- response surface experiment,
- amino acid composition

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[1]	徐永霞, 孟德飞, 赵洪雷, 等. 即食鲐鱼肉干的调味配方及微波-热风干燥工艺优化[J]. 食品工业科技,2021,42(16):171−176. [XU Y X, MENG D F, ZHAO H L, et al. Optimization of seasoning formula and microwave-hot air drying technology for ready-to-eat dried mackerel[J]. Science and Technology of Food Industry,2021,42(16):171−176. XU Y X, MENG D F, ZHAO H L, et al. Optimization of seasoning formula and microwave-hot air drying technology for ready-to-eat dried mackerel[J]. Science and Technology of Food Industry, 2021, 42(16): 171-176.
[2]	王晓龙. 鲐鱼鱼油提取精制及抗氧化活性研究[D]. 舟山: 浙江海洋大学, 2014. WANG X L. Extraction and purification of fish oil from mackerel processing byproduct and its antioxidant activity[D]. Zhoushan: Zhejiang Ocean University, 2014.
[3]	陈海桂, 王阳光. 酶解鲐鱼蛋白制取功能性鱼蛋白粉加工工艺研究[J]. 现代农业科技,2008(20):227−228. [CHEN H G, WANG Y G. Preparation of functional fish protein powder by enzymatic hydrolysis of mackerel protein[J]. Modern Agricultural Science and Technology,2008(20):227−228. doi: 10.3969/j.issn.1007-5739.2008.20.156 CHEN H G, WANG Y G. Preparation of functional fish protein powder by enzymatic hydrolysis of mackerel protein[J]. Xiandai Nong Ye Keji, 2008(20): 227-228. doi: 10.3969/j.issn.1007-5739.2008.20.156
[4]	ROCHA M D, ALEMAN A, BACCAN G, et al. Anti-inflammatory, antioxidant, and antimicrobial effects of underutilized fish protein hydrolysate[J]. Journal of Aquatic Food Product Technology,2018,27(5):592−608. doi: 10.1080/10498850.2018.1461160
[5]	李致瑜. 大黄鱼内脏抗氧化肽的制备, 分离纯化及其理化性质研究[D]. 福州: 福建农林大学, 2016. LI Z Y. Study on the preparation and purification of antioxidant peptides from large yellow croaker viscera protein and its structure and physicochemical properties[D]. Fuzhou: Fujian Agriculture and Forestry University, 2016.
[6]	江锟. 鲈鱼蛋白的酶解工艺优化及活性肽的功能特性研究[D]. 武汉: 华中农业大学, 2013. JIANG K. Studies of bass protein hydrolysis process optimization and active peptide functional properties[D]. Wuhan: Huazhong Agricultural University, 2013.
[7]	XU B, YE L, TANG Y, et al. Preparation and purification of an immunoregulatory peptide from Stolephorus chinensis of the East Sea of China[J]. Process Biochemistry,2020,98:151−159. doi: 10.1016/j.procbio.2020.08.011
[8]	LOURDES S L, ADRIAN H M, BELINDA V C, et al. Food-derived immunomodulatory peptides[J]. Journal of the Science of Food & Agriculture,2016,96:3631−3641.
[9]	LIU P, LIAO W, QI X, et al. Identification of immunomodulatory peptides from zeinhydrolysates[J]. European Food Research and Technology,2020,246(5):931−937. doi: 10.1007/s00217-020-03450-x
[10]	MERAM C, YU W L, WU J P. Immunomodulatory and anticancer protein hydrolysates (peptides) from food proteins: A review[J]. Food Chemistry,2018,245:205−222. doi: 10.1016/j.foodchem.2017.10.087
[11]	XU Z, MAO T M, HUANG L, et al. Purification and identification immunomodulatory peptide from rice protein hydrolysates[J]. Food and Agricultural Immunology,2019,30(1):150−162. doi: 10.1080/09540105.2018.1553938
[12]	纪丽娜. 金枪鱼头酶解物免疫活性肽的分离及对小鼠腹腔巨噬细胞功能的影响[D]. 湛江: 广东海洋大学, 2012. JI L N. Studies on separation of immune activity peptide from tuna head hydrolysates and its effect on mouse peritoneal macrophages[D]. Zhanjiang: Guangdong Ocean University, 2012.
[13]	侯虎. 鳕鱼免疫活性肽的可控制备及其免疫活性研究[D]. 青岛: 中国海洋大学, 2011. HOU H. The controllable preparation of pollock immunomodulating peptide and its immunity activity[D]. Qingdao: Ocean University of China, 2011.
[14]	邓志程. 马氏珠母贝全脏器免疫活性肽的制备及其免疫活性的研究[D]. 湛江: 广东海洋大学, 2015. DENG Z C. Study on the preparation of Pinctada martensii immunomodulating peptide and its immunity function[D]. Zhanjiang: Guangdong Ocean University, 2015.
[15]	房新平. 牛胎盘免疫活性肽提取与酶法制备研究[D]. 无锡: 江南大学, 2007. FANG X P. Studies on extraction and enzymatic preparation of the immunoactive peptides from bovine placenta[D]. Wuxi: Jiangnan University, 2007.
[16]	YANG R Y, ZHANG Z F, PEI X R, et al. Immunomodulatory effects of marine oligopeptide preparation from Chum Salmon (Oncorhynchus keta) in mice[J]. Food Chemistry,2009,113(2):464−470. doi: 10.1016/j.foodchem.2008.07.086
[17]	YANG Q, CAI X, HUANG M, et al. A specific peptide with immunomodulatory activity from Pseudostellaria heterophylla and the action mechanism[J]. Journal of Functional Foods,2020,68:103887. doi: 10.1016/j.jff.2020.103887
[18]	王雪芹. 鲐鱼多肽的抗氧化活性与抗疲劳作用研究[D]. 北京: 中国科学院大学, 2014. WANG X Q. Study on the antioxidant activity and antifatigue effect of mackerel peptides[D]. Beijing: University of Chinese Academy of Sciences, 2014.
[19]	中华人民共和国国家卫生和计划生育委员会. GB 5009.3-2016食品安全国家标准食品中水分的测定[S]. 北京: 中国标准出版社, 2016. National Health and Family Planning Commission of the People's Republic of China. GB 5009.3-2016 National food safety standards. Determination of moisture in food[S]. Beijing: China Standards Press, 2016.
[20]	中华人民共和国国家卫生和计划生育委员会. GB 5009.4-2016食品安全国家标准食品中灰分的测定[S]. 北京: 中国标准出版社, 2016. National Health and Family Planning Commission of the People's Republic of China. GB 5009.4-2016 National foodsafety standards. Determination of ash content in food[S]. Beijing: China Standards Press, 2016.
[21]	中华人民共和国国家卫生和计划生育委员会. GB 5009.5-2016食品安全国家标准食品中蛋白质的测定[S]. 北京: 中国标准出版社, 2016. National Health and Family Planning Commission of the People's Republic of China. GB 5009.5-2016 National foodsafety standards. Determination of protein in food[S]. Beijing: China Standards Press, 2016.
[22]	中华人民共和国国家卫生和计划生育委员会. GB 5009.6-2016食品安全国家标准食品中脂肪的测定[S]. 北京: 中国标准出版社, 2016. National Health and Family Planning Commission of the People's Republic of China. GB 5009.6-2016 Nationalfood safety standards. Determination of fat in food[S]. Beijing: China Standards Press, 2016.
[23]	. 胡旭阳. 日本黄姑鱼肉活性肽的制备及其免疫调节作用研究[D]. 舟山: 浙江海洋大学, 2019. HU X Y. Studies on the preparation and immunoregulatory effect of peptides from Nibea japonica[D]. Zhoushan: Zhejiang Ocean University, 2019.
[24]	中华人民共和国国家卫生和计划生育委员会. GB 5009.235-2016食品安全国家标准食品中氨基酸态氮的测定[S]. 北京: 中国标准出版社, 2016. National Health and Family Planning Commission of the People’s Republic of China. GB 5009.235-2016 National food safety standards. Determination of amino acids nitrogen in food[S]. Beijing: China Standards Press, 2016.
[25]	董晓泽. 日本黄姑鱼皮活性肽的制备及其免疫作用研究[D]. 舟山: 浙江海洋大学, 2019. DONG X Z. Preparation and immunological study of active peptides from Nibea japonica skin[D]. Zhoushan: Zhejiang Ocean University, 2019.
[26]	李志永. 蚕蛹蛋白免疫肽的分离纯化、结构鉴定及功能分析[D]. 镇江: 江苏科技大学, 2019. LI Z Y. Purification, identification, and functional analysis of the novel immunomodulatory peptides from silkworm pupa protein[D]. Zhenjiang: Jiangsu University of Science and Technology, 2019.
[27]	中华人民共和国国家卫生和计划生育委员会. GB 5009.124-2016食品安全国家标准食品中氨基酸的测定[S]. 北京: 中国标准出版社, 2016. National Health and Family Planning Commission of the People's Republic of China. GB 5009.124-2016 National food safety standards. Determination of amino acids in food[S]. Beijing: China Standards Press, 2016.
[28]	林凯, 韩雪, 张兰威, 等. ACE抑制肽构效关系及其酶法制备的研究进展[J]. 食品科学,2017,38(3):261−270. [LIN K, HAN X, ZHANG L W, et al. Progress in structure-activity relationship and enzymatic preparation of ACE inhibitory peptides[J]. Food Science,2017,38(3):261−270. LIN K, HAN X, ZHANG L W, et al. Progress in structure-activity relationship and enzymatic preparation of ACE inhibitory peptides[J]. Food Science, 2017, 38(3): 261-270.
[29]	屈帅杰, 刘淑集, 苏永昌, 等. 响应面法优化菊黄东方鲀肌肉多肽制备工艺[J]. 食品工业科技,2021,42(12):133−138. [QU S J, LIU S J, SU Y C, et al. Optimization of polypeptides extraction from Takifugu flavidus by response surface methodology[J]. Science and Technology of Food Industry,2021,42(12):133−138. QU S J, LIU S J, SU Y C, et al. Optimization of polypeptides extraction from Takifugu flavidus by response surface methodology[J]. Science and Technology of Food Industry, 2021, 42(12): 133-138.
[30]	程镇燕, 陈韶阳, 乔秀亭. 鱼类功能性氨基酸营养免疫研究进展[J]. 饲料研究,2014(9):53−57. [CHENG Z Y, CHEN S Y, QIAO X T. Research progress on nutritional immunity of functional amino acids in fish[J]. Feed Research,2014(9):53−57. CHENG Z Y, CHEN S Y, QIAO X T. Research progress on nutritional immunity of functional amino acids in fish[J]. Feed Research, 2014(9): 53-57.
[31]	LI W, YE S, ZHANG Z, et al. Purification and characterization of a novel pentadecapeptide from protein hydrolysates of Cyclinasinensis and its immunomodulatory effects on RAW264.7 cells[J]. Marine Drugs,2019,17(1):30. doi: 10.3390/md17010030
[32]	王凯凯. 绿豆肽的结构鉴定及对小鼠巨噬细胞免疫活性物质的影响作用研究[D]. 大庆: 黑龙江八一农垦大学, 2016. WANG K K. Structure identification of mung bean peptides and its effect on the immunoreactive substances of macrophage in mice[D]. Daqing: Heilongjiang Bayi Agricultural University, 2016.
[33]	张亚飞. 小麦蛋白Alcalase水解物中免疫活性肽的研究[D]. 无锡: 江南大学, 2008. ZHANG Y F. The study on immunopeptides from alcalase-hydrolyzed wheat proteins[D]. Wuxi: Jiangnan University, 2008.
[34]	杨磊. 螺旋藻活性肽的制备及其免疫调节功能研究[D]. 南宁: 广西大学, 2009. YANG L. Study on preparation of active peptides from spirulina mud and its immune regulating function[D]. Nanning: Guangxi University, 2009.

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