Research Progress on Structure-activity Relationship and Application of <i>α</i>-Amylase Inhibitors

LI Mengyang; CHANG Yuan; FENG Guojun; SUN Qingshen

doi:10.13386/j.issn1002-0306.2021080293

Volume 43 Issue 12

Jun. 2022

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Science and Technology of Food Industry > 2022 > 43(12): 475-485. > DOI: 10.13386/j.issn1002-0306.2021080293

LI Mengyang, CHANG Yuan, FENG Guojun, et al. Research Progress on Structure-activity Relationship and Application of α-Amylase Inhibitors[J]. Science and Technology of Food Industry, 2022, 43(12): 475−485. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021080293.

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Research Progress on Structure-activity Relationship and Application of α-Amylase Inhibitors

LI Mengyang^{1, 2,},
CHANG Yuan^{1, 2},
FENG Guojun^{1, 2, 3, ,},
SUN Qingshen^{1, 4, ,}

1.
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500, China
2.
Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080, China
3.
College of Advanced Agricultural, Ecological and Environmental Sciences, Heilongjiang University, Harbin 150080, China
4.
Key Laboratory of Molecular Biology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080, China

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Received Date: August 25, 2021
Available Online: April 13, 2022

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Abstract

Abstract

: α-amylase inhibitors (α-AI) are a kind of active substances which show inhibitory activities to human, insect pancreas and saliva. α-AI can reduce blood glucose index in human body and kill insects, thus widely used in medicine and agriculture. This review summarizes the source, structure and action mechanisms of protein and non-protein-based α-AI, focuses on the mechanisms from the view of structure-function relationship. The applications of α-AI in disease prevention, agricultural production and plant protection are also summarized. This review may provide new insights into the comprehensive exploitation and application of α-AI from different sources.
- α-amylase inhibitor,
- structure-activity relationships,
- classification,
- mechanisms of action,
- application

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References (71)

References

[1]	BARRETT M L, UDANI J K. A proprietary alpha-amylase inhibitor from white bean (Phaseolus vulgaris): A review of clinical studies on weight loss and glycemic control[J]. Nutrition Journal,2011,10:24. doi: 10.1186/1475-2891-10-24
[2]	孟鹏, 周秀秀. 链霉菌产α-葡萄糖苷酶和α-淀粉酶抑制剂等降糖药物研究概述[J]. 国外医药(抗生素分册),2016,37(4):145−153. [MENG P, ZHOU X X. Study on α-glucosidase and α-amylase inhibitors produced by Streptomyces[J]. World Notes on Antibiotics,2016,37(4):145−153. MENG P, ZHOU X X. Study on α-glucosidase and α-amylase inhibitors produced by Streptomyces[J]. World Notes on Antibiotics, 2016, 37(4): 145-153.
[3]	AISPURO-PEREZ A, LOPEZ-VALOS J, GARCIA-PAEZ F, et al. Synthesis and molecular docking studies of imines as α-glucosidase and α-amylase inhibitors[J]. Bioorganic Chemistry,2019,94:103491.
[4]	QIN G, WANG F, LIANG H, et al. Subchronic study of a white kidney bean (Phaseolus vulgaris) extract with alpha-amylase inhibitory activity[J]. Biomed Research International,2019,2019:9272345.
[5]	PRIYANKA R, YADAV P R. Anti-diabesity potential of various multifunctional natural molecules[J]. Journal of Herbal Medicine,2021,27:100430. doi: 10.1016/j.hermed.2021.100430
[6]	UDANI J, TAN O, MOLINA J. Systematic review and meta-analysis of a proprietary alpha-amylase inhibitor from white bean (Phaseolus vulgaris L.) on weight and fat loss in humans[J]. Foods,2018,7(4):63. doi: 10.3390/foods7040063
[7]	吕凤霞, 陆兆新. α-淀粉酶抑制剂的研究进展[J]. 食品科学,2002,23(3):152−155. [LYU F X, LU Z X. Research progress of α-amylase inhibitors[J]. Food Science,2002,23(3):152−155. doi: 10.3321/j.issn:1002-6630.2002.03.043 LV F X, LU Z X. Research progress of α-amylase inhibitors[J]. Food Science, 2002, 23(3): 152-155. doi: 10.3321/j.issn:1002-6630.2002.03.043
[8]	杨明琰. 白芸豆中α-淀粉酶抑制剂糖蛋白的提取纯化、组成结构及生物活性研究[D]. 西安: 西北大学, 2008. YANG M Y. Extraction, purification, structure and biological activity of α-amylase inhibitor glycoprotein from kidney bean[D]. Xi’an: Northwest University, 2008.
[9]	BEIDOKHTI M N, JAGER A K. Review of antidiabetic fruits, vegetables, beverages, oils and spicescommonly consumed in the diet[J]. Journal of Ethnopharmacology,2017,201:26−41. doi: 10.1016/j.jep.2017.02.031
[10]	PAPOUTSIS K, ZHANG J, BOWYER M C, et al. Fruit, vegetables, and mushrooms for the preparation of extracts with α-amylase and α-glucosidase inhibition properties: A review[J]. Food Chemistry,2021,338(11):128119.
[11]	魏鹏娟, 王鲁峰, 徐晓云, 等. α-淀粉酶蛋白类抑制剂的研究进展[J]. 食品科学,2011,32(9):312−318. [WEI P J, WANG L F, XYU X Y, et al. Research progress of α-amylase protein inhibitors[J]. Food Science,2011,32(9):312−318. WEI P J, WANG L F, XV X Y, et al. Research progress of α-amylase protein inhibitors[J]. Food Science, 2011, 32(9): 312-318.
[12]	寇秀颖. α-淀粉酶抑制剂制备方法研究进展[J]. 食品工业科技,2008,29(11):287−288. [KOU X Y. Research progress on preparation methods of α-amylase inhibitors[J]. Technology of Food Industry,2008,29(11):287−288. KOU X Y. Research progress on preparation methods of α-amylase inhibitors[J]. Technology of Food Industry, 2008, 29(11): 287-288.
[13]	VERTESY L, OEDING V, BENDER R, et al. Tendamistat (HOE 467), a tight-binding-amylase inhibitor from Streptomyces tendae 4158: Isolation, biochemical properties[J]. European Journal of Biochemistry,1984,141:505−512. doi: 10.1111/j.1432-1033.1984.tb08221.x
[14]	孙庆申, 朱国庆, 王璇, 等. 紫冠豆角(Phaseolus vulgaris L. )种子中α-淀粉酶抑制剂的提取及其性质研究[J]. 食品工业科技, 42(6): 137-143. SUN Q S, ZHU G Q, WANG X, et al. Extraction and properties of α-amylase inhibitors from Phaseolus vulgaris L. seeds[J]. Technology of Food Industry, 42(6): 137-143.
[15]	THAYUMANAVAN S. Inhibition of insect pest α-amylases by little and finger millet inhibitors[J]. Pesticide Biochemistry and Physiology,2006,85:155−160. doi: 10.1016/j.pestbp.2005.11.008
[16]	贾光锋. α-淀粉酶抑制剂的制备及检测[J]. 食品与药品,2007,9(2):34−36. [JIA G F. Preparation and determination of α-amylase inhibitor[J]. Food and Drug,2007,9(2):34−36. doi: 10.3969/j.issn.1672-979X.2007.02.013 JIA G F. Preparation and determination of α-amylase inhibitor[J]. Food and Drug, 2007, 9(2): 34-36. doi: 10.3969/j.issn.1672-979X.2007.02.013
[17]	刘华珍. 科科鲁链霉菌产生的新的蛋白质α-淀粉酶抑制剂[J]. 国外药学(抗生素分册),1985,47(2):315−316. [LIU H Z. New protein α-amylase inhibitor produced by Streptomyces corrugus[J]. World Notes on Antibiotics,1985,47(2):315−316. LIU H Z. New Protein α-amylase inhibitor produced by Streptomyces corrugus[J]. World Notes on Antibiotics, 1985, 47(2): 315-316.
[18]	刘华珍, 王嶽, 刘锜英. 微生物产生的酶抑制剂研究——Ⅴ. 产生淀粉酶抑制剂的链霉菌属的一个新变种[J]. 中国抗生素杂志,1985,10(5):281−284. [LIU H Z, WANG Y, LIU Q Y. Study on enzyme inhibitors produced by microorganisms —V. A new variety of Streptomyces producing amylase inhibitors[J]. Chinese Journal of Antibiotics,1985,10(5):281−284. LIU H Z, WANG Y, LIU Q Y. Study on enzyme inhibitors produced by microorganisms — V. A new variety of Streptomyces producing amylase inhibitors[J]. Chinese Journal of Antibiotics, 1985, 10(5): 281-284.
[19]	刘英, 刘华珍, 江宏磊. 微生物产生的淀粉酶抑制剂研究Ⅲ淀粉酶抑制剂抑制动力学研究[J]. 中国抗生素杂志,1994,19(1):17−20. [LIU Y, LIU H Z, JIANG H L. Study on amylase inhibitors produced by microorganisms III inhibition kinetics of amylase inhibitors[J]. Chinese Journal of Antibiotics,1994,19(1):17−20. LIU Y, LIU H Z, JIANG H L. Study on amylase inhibitors produced by microorganisms III inhibition kinetics of amylase inhibitors[J]. Chinese Journal of Antibiotics, 1994, 19(1): 17-20.
[20]	谢丽源. 菌源性α-淀粉酶抑制剂研究进展[J]. 四川食品与发酵,2002,38(4):14−16. [XIE L Y. Research progress of fungal α-amylase inhibitors[J]. Sichuan Food and Fermentation,2002,38(4):14−16. XIE L Y. Research progress of fungal α-amylase inhibitors[J]. Sichuan Food and Fermentation, 2002, 38(4): 14-16.
[21]	MENG P, GUO Y, ZHANG Q, et al. A novel amino-oligosaccharide isolated from the culture of Streptomyces strain PW638 is a potent inhibitor of α-amylase[J]. Carbohydrate Research,2011,346(13):1898−1902. doi: 10.1016/j.carres.2011.06.005
[22]	耿鹏. 天蓝黄链霉菌及其产生的新型α-淀粉酶抑制剂研究[D]. 天津: 南开大学, 2008. GENG P. Study on Streptomyces coelicolor and its new α-amylase inhibitor[D]. Tianjin: Nankai University, 2008.
[23]	王顺, 高瑞昶. 产α-淀粉酶抑制剂的药用植物内生放线菌的筛选[J]. 化工进展,2015,34(2):500−507,518. [WANG S, GAO R C. Screening of endophytic actinomycetes from medicinal plants producing α-amylase inhibitors[J]. Chemical Industry and Engineering Progress,2015,34(2):500−507,518. WANG S, GAO R C. Screening of endophytic actinomycetes from medicinal plants producing α-amylase inhibitors[J]. Chemical Industry and Engineering Progress, 2015, 34(2): 500-507, 518.
[24]	孙志斌. 产纤维蛋白酶和淀粉酶抑制剂放线菌Streptomyces sp. CC5的筛选及其产物的特性研究[D]. 南京: 南京农业大学, 2016. SUN Z B. Screening of Streptomyces sp. CC5 producing protease and amylase inhibitors and study of its characteristics[D]. Nanjing: Nanjing Agricultural University, 2016.
[25]	FRANCOISE P. Structural basis for the inhibition of mammalian and insect alpha-amylases by plant protein inhibitors[J]. Biochimica Et Biophysica Acta,2004,1696(2):171−180. doi: 10.1016/j.bbapap.2003.10.012
[26]	SALES P M, SOUZA P M, SIMEONI L A, et al. α-Amylase inhibitors: A review of raw material and isolated compounds from plant source[J]. Journal of Pharmacy & Pharmaceutical Sciences,2012,15(1):141−183.
[27]	POOVITHA S, PARANI M. In vitro and in vivo α-amylase and α-glucosidase inhibiting activities of the protein extracts from two varieties of bitter gourd (Momordica charantia L.)[J]. BMC Complementary and Alternative Medicine,2016,16(1):1−8.
[28]	CHEN X, HE X, ZHANG B, et al. Wheat gluten protein inhibits α-amylase activity more strongly than a soy protein isolate based on kinetic analysis[J]. International Journal of Biological Macromolecules,2019,129(1):433−441.
[29]	CHAGPLLALOPEZ A, BLANCOLABRA A, PATTHY A, et al. A novel α-amylase inhibitor from amaranth (Amaranthus hypocondriacus) seeds[J]. Journal of Biological Chemistry,1994,269(38):23675. doi: 10.1016/S0021-9258(17)31568-5
[30]	SUN L, WARREN F J, GIDLEY M. Natural products for glycaemic control: Polyphenols as inhibitors of alpha-amylase[J]. Trends in Food Science & Technology,2019,91(7):262−273.
[31]	XIAO J, NI X, KAI G, et al. A review on structure–activity relationship of dietary polyphenols inhibiting α-amylase[J]. Critical Reviews in Food Science and Nutrition,2013,53(5):497−506. doi: 10.1080/10408398.2010.548108
[32]	GOMES J, MBIAKOP U C, OLIVEIRA R L, et al. Polyphenol-rich extract and fractions of Terminalia phaeocarpa eichler possess hypoglycemic effect, reduce the release of cytokines, and inhibit lipase, α-glucosidase, and α-amylase enzymes[J]. Journal of Ethnopharmacology,2021:113847.
[33]	FIGUEIREDO-GONZÁLEZ M, GROSSO C, VALENTÃO P, et al. α-Glucosidase and α-amylase inhibitors from Myrcia spp. : A stronger alternative to acarbose?[J]. Journal of Pharmaceutical and Biomedical Analysis,2016,118:322−327. doi: 10.1016/j.jpba.2015.10.042
[34]	TKACZ K, WOJDYLO A, TURKIEWICZ I P, et al. Anti-oxidant and anti-enzymatic activities of sea buckthorn (Hippophaë rhamnoides L.) fruits modulated by chemical components[J]. Antioxidants,2019,8(12):1−17.
[35]	WANG K J, ZHAO J L, et al. Corn silk (Zea mays L.), a source of natural antioxidants with α-amylase, α-glucosidase, advanced glycation and diabetic nephropathy inhibitory activities[J]. Biomedicine & Pharmacotherapy,2018,110:510−517.
[36]	TADERA K, MINAMI Y, TAKAMATSU K, et al. Inhibition of α-glucosidase and α-amylase by flavonoids[J]. Journal of Nutritional Ence and Vitaminology,2007,52(2):149−153.
[37]	ZHENG Y, TIAN J, YANG W, et al. Inhibition mechanism of ferulic acid against α-amylase and α-glucosidase[J]. Food Chemistry,2020,317:126346. doi: 10.1016/j.foodchem.2020.126346
[38]	SALES P M D, SOUZA P M D, DARTORA M, et al. Pouteria torta epicarp as a useful source of α-amylase inhibitor in the control of type 2 diabetes[J]. Food and Chemical Toxicology,2017,109:962−969. doi: 10.1016/j.fct.2017.03.015
[39]	ZAHARUDIN N, SALMEAN A A, DRAGSTED L O. Inhibitory effects of edible seaweeds, polyphenolics and alginates on the activities of porcine pancreatic α-amylase[J]. Food Chemistry,2018,245(15):1196.
[40]	ELENA L P, HOLGER S, NATHALIE F, et al. Flavonoids for controlling starch digestion: Structural requirements for inhibiting human alpha-amylase[J]. Journal of Medicinal Chemistry,2008,51(12):3555−3561. doi: 10.1021/jm800115x
[41]	TODOROVA T Z, TRAYKOV M G, TADJER A V, et al. Structure of flavones and flavonols. Part I: Role of substituents on the planarity of the system[J]. Computational and Theoretical Chemistry,2013,1017(13):85−90.
[42]	HUI X D, GANG W, DUO H, et al. The effects of bioactive compounds from blueberry and blackcurrant powders on the inhibitory activities of oat bran pastes against α-amylase and α-glucosidase linked to type 2 diabetes[J]. Food Research International,2020,138:109756. doi: 10.1016/j.foodres.2020.109756
[43]	TIAN J L, SI X, WANG Y H, et al. Bioactive flavonoids from rubus corchorifolius inhibit α-glucosidase and α-amylase to improve postprandial hyperglycemia[J]. Food Chemistry,2020,341:128149.
[44]	ANIGBORO A A, AVWIOROKO O J, OHWOKEVWO O A, et al. Phytochemical profile, antioxidant, α-amylase inhibition, binding interaction and docking studies of Justicia carnea bioactive compounds with α-amylase[J]. Biophysical Chemistry,2020,269:106529.
[45]	QUARESMA D M O, JUSTINO A B, SOUSA R M F, et al. Antioxidant compounds from Banisteriopsis argyrophylla leaves as α-amylase, α-glucosidase, lipase, and glycation inhibitors[J]. Bioorganic Chemistry,2020,105:104335. doi: 10.1016/j.bioorg.2020.104335
[46]	HUANG Y M, PENG W, JIAN Y B, et al. Mechanistic study on inhibition of porcine pancreatic α-amylase using the flavonoids from dandelion[J]. Food Chemistry,2020,344:128610.
[47]	LIU S, LI D, HUANG B, et al. Inhibition of pancreatic lipase, α-glucosidase, α-amylase, and hypolipidemic effects of the total flavonoids fromNelumbo nucifera leaves[J]. Journal of Ethnopharmacology,2013,149(1):263−269. doi: 10.1016/j.jep.2013.06.034
[48]	WANG M, SHI J, WANG L, et al. Inhibitory kinetics and mechanism of flavonoids from lotus (Nelumbo nucifera.Gaertn) leaf against pancreatic α-amylase[J]. International Journal of Biological Macromolecules,2018,120:2589−2596. doi: 10.1016/j.ijbiomac.2018.09.035
[49]	KWON Y I, APOSTOLIDIS E, SHETTY K. In vitro studies of eggplant (Solanum melongena) phenolics as inhibitors of key enzymes relevant for type 2 diabetes and hypertension[J]. Bioresource Technology,2008,99(8):.2981−2988. doi: 10.1016/j.biortech.2007.06.035
[50]	HYUK J J, EUN P J, HAN J S. Scopoletin inhibits α-glucosidase in vitro and alleviates postprandial hyperglycemia in mice with diabetes[J]. European Journal of Pharmacology,2018,834:152−156. doi: 10.1016/j.ejphar.2018.07.032
[51]	RASOULI H, YARANI R, POCIOT F, et al. Anti-diabetic potential of plant alkaloids: Revisiting current findings and future perspectives[J]. Pharmacological Research,2020,155:104723. doi: 10.1016/j.phrs.2020.104723
[52]	OCHIENG C O, NYONGESA D W, YAMO K O, et al. α-Amylase and α-glucosidase inhibitors from Zanthoxylum chalybeum Engl. root bark[J]. Fitoterapia,2020,146(12):104719.
[53]	AGATONOVIC-KUSTRIN S, KUSTRIN E, GEGECHKORI V, et al. Bioassay-guided identification of α-amylase inhibitors in herbal extracts[J]. Journal of Chromatography A,2020,1620:460970. doi: 10.1016/j.chroma.2020.460970
[54]	HARA Y, HONDA M. The inhibition of α-amylase by tea polyphenols[J]. Japan Society for Bioscience, Biotechnology, and Agrochemistry,1990,54(8):1939−1945.
[55]	D'COSTA A S, BORDENAVE N. Inhibition of starch digestion by flavonoids: Role of flavonoid-amylase binding kinetics[J]. Food Chemistry,2021,341(2):128256.
[56]	BERNAL C A, CASTELLANOS L, ARAGON D M, et al. Peruvioses A to F, sucrose esters from the exudate of Physalis peruviana fruit as α-amylase inhibitors[J]. Carbohydrate Research,2018,461:4−10. doi: 10.1016/j.carres.2018.03.003
[57]	TUNDIS R, MENICHINI F, BONESI M, et al. Antioxidant and hypoglycaemic activities and their relationship to phytochemicals in Capsicum annuum cultivars during fruit development[J]. LWT-Food Science and Technology,2013,53(1):370−377. doi: 10.1016/j.lwt.2013.02.013
[58]	CANSIGNO C L, HERNANDEZ-DOMINGUEZ E, LICEA A, et al. Screening of Mexican tropical seaweeds as sources of α-amylase and α-glucosidase inhibitors[J]. Algal Research,2020,49:101954. doi: 10.1016/j.algal.2020.101954
[59]	DAOUDI N E, BOUHRIM M, OUASSOU H, et al. Inhibitory effect of roasted/unroasted Argania spinosa seeds oil on α-glucosidase, α-amylase and intestinal glucose absorption activities[J]. South African Journal of Botany,2020,135:413−420. doi: 10.1016/j.sajb.2020.09.020
[60]	OGASAWARA M, YOSHII K, WADA J, et al. Identification of guanine, guanosine, and inosine for α-amylase inhibitors in the extracts of the earthworm Eisenia fetida and characterization of their inhibitory activities against porcine pancreatic α-amylase[J]. Enzyme and Microbial Technology,2020,142:109693. doi: 10.1016/j.enzmictec.2020.109693
[61]	龙楚媚, 付强, 王琪, 等. 罗汉果内生菌的分离及α-淀粉酶抑制剂产生菌的筛选[J]. 中国酿造,2019,38(1):71−74. [LONG C M, FU Q, WANG Q, et al. Isolation of endophytic bacteria from Siraitia grosvenorii and screening of α-amylase inhibitor producing bacteria[J]. China Brewing,2019,38(1):71−74. doi: 10.11882/j.issn.0254-5071.2019.01.014 LONG C M, FU Q, WANG Q, et al. Isolation of endophytic bacteria from Siraitia grosvenorii and screening of α-amylase inhibitor producing bacteria[J]. China Brewing, 2019, 38(1): 71-74. doi: 10.11882/j.issn.0254-5071.2019.01.014
[62]	杨宁, 赵燕云, 施文彩, 等. 进餐前服用白芸豆提取物对餐后血糖的改善功效[J]. 医学研究杂志,2020,49(4):24−28. [YANG N, ZHAO Y Y, SHI W C, et al. The improvement effect of white kidney bean extract on blood glucose after meal before meal[J]. Journal of Medical Research,2020,49(4):24−28. YANG N, ZHAO Y Y, SHI W C, et al. The improvement effect of white kidney bean extract on blood glucose after meal before meal[J]. Journal of Medical Research, 2020, 49(4): 24-28.
[63]	叶晓平, 宋纯清, 袁萍, 等. 糖尿病常用中药中常见成分的α-葡萄糖苷酶和α-淀粉酶活性抑制作用[J]. 中国天然药物,2010,8(5):349−352. [YE X P, SONG C Q, YUAN P, et al. Inhibitory effects of α-glucosidase and α-amylase activities of common components in traditional Chinese medicine for diabetes[J]. Chinese Journal of Natural Medicines,2010,8(5):349−352. doi: 10.1016/S1875-5364(10)60041-6 YE X P, SONG C Q, YUAN P, et al. Inhibitory effects of α-glucosidase and α-amylase activities of common components in traditional Chinese medicine for diabetes[J]. Chinese Journal of Natural Medicines, 2010, 8(5): 349-352. doi: 10.1016/S1875-5364(10)60041-6
[64]	于雪慧. 新疆奶花芸豆中α-淀粉酶抑制剂及凝集素的提取、分离、鉴定[D]. 石河子: 石河子大学, 2018. YU X H. Extraction, Isolation and identification of α-amylase inhibitor and lectin from Xinjiang Dairy Bean[D]. Shihezi: Shihezi University, 2018.
[65]	SHI Z X, ZHU Y Y, TENG C, et al. Anti-obesity effects of α-amylase inhibitor enriched-extract from white common beans (Phaseolus vulgaris L.) associated with the modulation of gut microbiota composition in high-fat diet-induced obese rats[J]. Food & Function,2020,11(2):1624−1634.
[66]	薛士科, 冯利. 红芸豆α-淀粉酶抑制剂和中草药组合饲料的应用价值[J]. 中国饲料,2020,24(3):52−54. [XUE S K, FENG L. Application value of α-amylase inhibitor of red kidney bean and Chinese herbal compound feed[J]. China Feed,2020,24(3):52−54. XUE S K, FENG L. Application value of α-amylase inhibitor of red kidney bean and Chinese herbal compound feed[J]. China Feed, 2020, 24(3), 52-54.
[67]	GONZALEZ-ROIZ C R, TORO-SANCHEZ C L, CORNEJO-RAMIREZ Y I, et al. Differential biochemical and kinetic properties of α-amylases from Rhyzopertha dominica (F.) progenies reared on wheat varieties differing in α-amylase inhibitory activity[J]. Journal of Stored Products Research,2021,90:101748. doi: 10.1016/j.jspr.2020.101748
[68]	RANI A, CHAND S, THAKUR N, et al. Alpha-amylase inhibitor from local common bean selection: Effect on growth and development of Corcyra cephalonica[J]. Journal of Stored Products Research,2018,75:35−37. doi: 10.1016/j.jspr.2017.10.009
[69]	GADGE P P, WAGH S K, SHAIKH F K, et al. A bifunctional α-amylase/trypsin inhibitor from pigeonpea seeds: Purification, biochemical characterization and its bio-efficacy against Helicoverpa armigera[J]. Pesticide Biochemistry & Physiology,2015,125:17−25.
[70]	PEREIRA R, BATISTA J A N, SILVA M C M, et al. An a-amylase inhibitor gene from Phaseolus coccineus encodes a protein with potential for control of coffee berry borer (Hypothenemus hampei)[J]. Phytochemistry,2006,67(18):2009−2016. doi: 10.1016/j.phytochem.2006.06.029
[71]	王琳. 昆虫淀粉酶抑制剂的研究进展[J]. 中国农学通报,2006,22(8):397−400. [WANG L. Research progress of insect amylase inhibitors[J]. Chinese Agricultural Science Bulletin,2006,22(8):397−400. doi: 10.3969/j.issn.1000-6850.2006.08.100 WANG L. Research progress of insect amylase inhibitors[J]. Chinese Agricultural Science Bulletin, 2006, 22(8): 397-400. doi: 10.3969/j.issn.1000-6850.2006.08.100