Quenching Effects of <i>Lactobacillus pentosus</i> DF9 on Quorum Sensing of <i>Vibrio parahaemolyticus</i>

SHANGGUAN Wendan; CHEN Ke; CHEN Qingying; ZHANG Xingguo; ZHONG Qingping

doi:10.13386/j.issn1002-0306.2021070267

Volume 43 Issue 6

Mar. 2022

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2022 > 43(6): 126-132. > DOI: 10.13386/j.issn1002-0306.2021070267

SHANGGUAN Wendan, CHEN Ke, CHEN Qingying, et al. Quenching Effects of Lactobacillus pentosus DF9 on Quorum Sensing of Vibrio parahaemolyticus[J]. Science and Technology of Food Industry, 2022, 43(6): 126−132. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070267.

Citation:

PDF (5233 KB)

Quenching Effects of Lactobacillus pentosus DF9 on Quorum Sensing of Vibrio parahaemolyticus

SHANGGUAN Wendan^{1, 2,},
CHEN Ke^{1, 2},
CHEN Qingying^{1, 2},
ZHANG Xingguo^{1, 2},
ZHONG Qingping^{1, 2, ,}

1.
SCAU (Chaozhou) Food Institute Co., Ltd., Chaozhou 521000, China
2.
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China

More Information

Received Date: July 22, 2021
Available Online: January 12, 2022

Graphical Abstract

Abstract

Abstract

The quenching effects of ethyl acetate extract of Lactobacillus pentosus DF9 as quorum sensing inhibitor on the quorum sensing (QS) of Vibrio parahaemolyticus were studied. Based on determining the effects of DF9-QSI on the growth of V. parahaemolyticus and V. harveyi BB170, the effects of DF9-QSI at sub-inhibitory concentration on QS signal molecule AI-2 activity, motility (swarming, swimming and twitching), biofilm formation and extracellular polysaccharide (EPS) synthesis of V. parahaemolyticus were investigated. The results showed that DF9-QSI had no effect on the growth of V. parahaemolyticus at the concentration lower than 0.8 mg/mL, but effectively reduced the AI-2 activity, and inhibited the movement, biofilm formation and EPS synthesis of V. parahaemolyticus in a concentration-dependent manner. The destructive effects of DF9-QSI on biofilms were further confirmed by the observations using optical microscope, scanning electron microscope and laser confocal microscope. These results indicated that DF9-QSI possessed AI-2 QS quenching activity, which might provide the theoretical basis for developing a novel QSI from lactic acid bacteria, and supply the potential microbial resources for controlling QS system of V. parahaemolyticus.
- Vibrio parahaemolyticus,
- Lactobacillus pentosus,
- quorum sensing inhibitor,
- biofilm

FullText(HTML)

References (30)

References

[1]	DANIEL P, SCHOFIELD M C, PARSEK M R, et al. An update on the sociomicrobiology of quorum sensing in gram-negative biofilm development[J]. Pathogens (Basel),2017,6(4):51. doi: 10.3390/pathogens6040051
[2]	MIZAN M F R, JAHID I K, HA S. Microbial biofilms in seafood: A food-hygiene challenge[J]. Food Microbiology,2015,49:41−55. doi: 10.1016/j.fm.2015.01.009
[3]	ASHRAFUDOULLA M, MIZAN M F R, PARK H, et al. Genetic relationship, virulence factors, drug resistance profile and biofilm formation ability of Vibrio parahaemolyticus isolated from mussel[J]. Frontiers in Microbiology,2019,10:513. doi: 10.3389/fmicb.2019.00513
[4]	LI Y, YIN H Q, XIA J, et al. Population structure and genetic diversity of Vibrio parahaemolyticus from a coastal area of China based on a multi-locus sequence typing (MLST) scheme[J]. Antonie Van Leeuwenhoek,2019,112(8):1199−1211. doi: 10.1007/s10482-019-01252-0
[5]	MARTINEZ-URTAZA J, VAN AERLE R, ABANTO M, et al. Genomic variation and evolution of Vibrio parahaemolyticus ST36 over the course of a transcontinental epidemic expansion[J]. mBio,2017,8(6):e01425−17-17.
[6]	MUKHERJEE S, BASSLER B L. Bacterial quorum sensing in complex and dynamically changing environments[J]. Nature Reviews Microbiology,2019,17(6):371−382. doi: 10.1038/s41579-019-0186-5
[7]	GUO M, FANG Z, SUN L, et al. Regulation of thermostable direct hemolysin and bofilm formation of Vibrio parahaemolyticus by quorum-sensing genes luxM and luxS[J]. Current Microbiology,2018,75(9):1190−1197. doi: 10.1007/s00284-018-1508-y
[8]	张义全. AphA和OpaR对副溶血弧菌密度感应系统相关基因的转录调控机制研究[D]. 北京: 中国人民解放军军事医学科学院, 2014 ZHANG Yiquan. Transcriptional regulation of the genes associated with quorum sensing system by AphA and OpaR in Vibrio parahaemolyticus[D]. Beijing: Academy of Military Medical Sciences, 2014.
[9]	蒋富凤, 雷涛, 吴清平, 等. 副溶血性弧菌生物被膜形成及其调控机制研究进展[J]. 微生物学报,2020,60(11):2381−2390. [JIANG Fufeng, LEI Tao, WU Qingping et al. Biofilm formation and regulation mechanism of Vibrio parahaemolyticus: Progress and trends[J]. Acta Microbiologica Sinica,2020,60(11):2381−2390.
[10]	张璐, 桂萌, 李平兰. 食品防腐中群体感应及其抑制剂的研究进展[J]. 食品工业科技,2016,37(3):364−367. [ZHANG Lu, GUI Meng, LI Pinglan. Application of quorum sensing inhibitors in food preservation[J]. Science and Technology of Food Industry,2016,37(3):364−367.
[11]	吴雅茜, 刘颖, 张瀛, 等. 4-羟基-2, 5-二甲基-3(2H)呋喃酮对单增李斯特菌AI-2类群体感应的干扰效应[J]. 食品工业科技,2018,39(22):87−92. [WU Yaqian, LIU Ying, ZHANG Ying, et al. Interferce effect of 4-hydroxy-2, 5-dimethyl-3(2H)-furanone on AI-2 quorum wensing of Listeria monocytogenes[J]. Science and Technology of Food Industry,2018,39(22):87−92.
[12]	于淑池, 邢文君, 冯紫蓝, 等. 基于群体感应研究苦丁茶多酚对荧光假单胞菌腐败特性的抑制作用[J]. 食品工业科技,2021,42(21):148−157. [YU Shuchi, XING Wenjun, FENG Zilan, et al. Inhibition of Kuding tea polyphenols against the spoilage characteristics of Pseudomonas fluorescens based on quorum sensing[J]. Science and Technology of Food Industry,2021,42(21):148−157.
[13]	GOMEZ N C, RAMIRO J M, QUECAN B X, et al. Use of potential probiotic lactic acid bacteria (LAB) biofilms for the control of Listeria monocytogenes, Salmonella typhimurium, and Escherichia coli O157: H7 biofilms formation[J]. Frontiers in Microbiology,2016,7:863.
[14]	PARK H, PARK S, YEO S, et al. Autoinducer-2 associated inhibition by Lactobacillus sakei NR28 reduces virulence of enterohaemorrhagic Escherichia coli O157: H7[J]. Food Control,2014,45:62−69. doi: 10.1016/j.foodcont.2014.04.024
[15]	黄湘湄, 吴雅茜, 刘颖, 等. 海洋源乳酸菌AI-2类群体感应抑制剂对单增李斯特菌抑制效果研究[J]. 生物技术通报,2019,35(4):36−42. [HUANG Liangxiang, WU Yaqian, LIU Ying, et al. Inhibitory effects of AI-2 quorum sensing inhibitors from marine lactic acid bacteria on Listeria monocytogenes[J]. Biotechnology Bulletin,2019,35(4):36−42.
[16]	BASSLER B L, GREENBERG E P, STEVENS A M. Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi[J]. Journal of Bacteriology,1997,179(12):4043−4045. doi: 10.1128/jb.179.12.4043-4045.1997
[17]	PELYUNTHA W, CHAIYASUT C, KANTACHOTE D, et al. Cell-free supernatants from cultures of lactic acid bacteria isolated from fermented grape as biocontrol against Salmonella typhi and Salmonella typhimurium virulence via autoinducer-2 and biofilm interference[J]. PeerJ,2019,7:e7555. doi: 10.7717/peerj.7555
[18]	O TOOLE G A, WONG G C. Sensational biofilms: Surface sensing in bacteria[J]. Current Opinion in Microbiology,2016,30:139−146. doi: 10.1016/j.mib.2016.02.004
[19]	VERSTRAETEN N, BRAEKEN K, DEBKUMARI B, et al. Living on a surface: Swarming and biofilm formation[J]. Trends in Microbiology (Regular ed. ),2008,16(10):496−506.
[20]	KOKARE C, CHAKRABORTY S, KHOPADE A, et al. Biofilm: Importance and applications[J]. Indian Journal of Biotechnology,2009,8(2):159−168.
[21]	ENOS-BERLAGE J L, GUVENER Z T, KEENAN C E, et al. Genetic determinants of biofilm development of opaque and translucent Vibrio parahaemolyticus[J]. Mol Microbiol,2005,55(4):1160−1182.
[22]	林洋, 孙梦桐, 吕欣然, 等. 滤纸片法筛选抑制嗜水气单胞菌群体感应的乳酸菌及抑制作用分析[J]. 食品科学,2018,39(14):125−131. [LIN Yang, SUN Mengtong, LU Xinran, et al. Screening of lactic acid bacteria with inhibitory activity against quorum sensing of Aeromonas hydrophila using filter paper method and their inhibitory effects[J]. Food Science,2018,39(14):125−131. doi: 10.7506/spkx1002-6630-201814019
[23]	孙梦桐, 吕欣然, 林洋, 等. 传统腌渍蔬菜中抑制嗜水气单胞菌群体感应及生物膜形成乳酸菌的筛选[J]. 中国食品学报,2020,20(7):172−180. [SUN Mengtong, LU Xinran, LIN Yang, et al. Screening of lactic acid bacteria inhibiting quorum sensing and biofilm of Aeromonas hydrophila in Chinese traditional pickled vegetables[J]. Journal of Chinese Institute of Food Science and Technology,2020,20(7):172−180.
[24]	孙梦桐, 吕欣然, 崔天琦, 等. 鱼类肠道中抑制哈维氏弧菌群体感应及生物膜形成的乳酸菌的筛选和鉴定[J]. 中国食品学报,2020,20(8):255−263. [SUN Mengtong, LU Xinran, CUI Tianqi, et al. Screening and identification of lactic acid bacteria with inhibitory activity of quorum sensing and biofilm of Vibrio harveyi from fish intestine[J]. Journal of Chinese Institute of Food Science and Technology,2020,20(8):255−263.
[25]	YILDIZ F H, VISICK K L. Vibrio biofilms: So much the same yet so different[J]. Trends in Microbiology (Regular ed.),2008,17(3):109−118.
[26]	SREY S, JAHID I K, HA S. Biofilm formation in food industries: A food safety concern[J]. Food Control,2013,31(2):572−585. doi: 10.1016/j.foodcont.2012.12.001
[27]	NITHYA C, DEVI M G, KARUTHA PANDIAN S. A novel compound from the marine bacterium Bacillus pumilus S6-15 inhibits biofilm formation in Gram-positive and Gram-negative species[J]. Biofouling (Chur, Switzerland),2011,27(5):519−528.
[28]	SANTHAKUMARI S, KANNAPPAN A, PANDIAN S K, et al. Inhibitory effect of marine cyanobacterial extract on biofilm formation and virulence factor production of bacterial pathogens causing vibriosis in aquaculture[J]. Journal of Applied Phycology,2016,28(1):313−324. doi: 10.1007/s10811-015-0554-0
[29]	XIE T, LIAO Z, LEI H, et al. Antibacterial activity of food-grade chitosan against Vibrio parahaemolyticus biofilms[J]. Microbial Pathogenesis,2017,110:291−297. doi: 10.1016/j.micpath.2017.07.011
[30]	SANTHAKUMARI S, JAYAKUMAR R, LOGALASHMI R, et al. In vitro and in vivo effect of 2, 6-Di-tert-butyl-4-methylphenol as an antibiofilm agent against quorum sensing mediated biofilm formation of Vibrio spp[J]. Int J Food Microbiol,2018,281:60−71. doi: 10.1016/j.ijfoodmicro.2018.05.024

Supplements (0)

Cited By

Cited by

Periodical cited type(2)

1.	叶国良，张杏果，黄铭新，李婷，钟青萍. 戊糖乳杆菌Z097提取物对单增李斯特菌群体感应信号分子和生物被膜的抑制作用. 食品与发酵工业. 2025(03): 64-71 .
2.	袁硕，王彦博，刘水琳，崔方超，檀茜倩，吕欣然，励建荣，李学鹏，董浩. 高产AI-2副溶血性弧菌的筛选及毒力特性研究. 中国食品学报. 2025(02): 115-123 .