Optimization of Extraction Method of <i>Lycium barbarum</i> Flavonoids and the Correlation between Different Ecological Factors and <i>Lycium barbarum</i> Flavonoids

WANG Pengbo; XIE Xiaorong; DAI Yunyun; LIU Jianshu; CHEN Mei; ZHANG Xia; PEI Dong; DI Duolong

doi:10.13386/j.issn1002-0306.2021070249

Volume 43 Issue 6

Mar. 2022

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Science and Technology of Food Industry > 2022 > 43(6): 236-242. > DOI: 10.13386/j.issn1002-0306.2021070249

WANG Pengbo, XIE Xiaorong, DAI Yunyun, et al. Optimization of Extraction Method of Lycium barbarum Flavonoids and the Correlation between Different Ecological Factors and Lycium barbarum Flavonoids[J]. Science and Technology of Food Industry, 2022, 43(6): 236−242. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070249.

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Optimization of Extraction Method of Lycium barbarum Flavonoids and the Correlation between Different Ecological Factors and Lycium barbarum Flavonoids

WANG Pengbo^{1, 2,},
XIE Xiaorong^1, ,,
DAI Yunyun^{1, 2},
LIU Jianshu³,
CHEN Mei³,
ZHANG Xia²,
PEI Dong^2, ,,
DI Duolong^{1, 2}

1.
School of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China
2.
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences Key Laboratory of Northwestern Featured Plant Resources Chemistry, Chinese Academy of Sciences and Key Laboratory of Natural Medicines of Gansu Province, Lanzhou 730000, China
3.
Functional Food Engineering Center of Shaanxi Province, Xi’an 710000, China

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Received Date: July 21, 2021
Available Online: January 13, 2022

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Abstract

Abstract

Lycium barbarum flavone is the main effective component of Chinese medicinal material Lycium barbarum, and it is also the main evaluation index of its quality. This paper investigated the effects of ethanol reflux extraction, high-speed shear-assisted ethanol extraction, high-speed shear-assisted DESs extraction, and ultrasound-assisted DESs extraction on the extraction amount of Lycium barbarum flavonoids. The best extraction method was selected, and the best extraction conditions were obtained by single factor experiments and orthogonal experiment. Based on the best extraction method, SIMCA 14.1 was used. The software analyzed the correlation between the ecological factors in different producing areas and the content of Lycium barbarum flavonoids. The results showed that the extraction efficiency of Lycium barbarum flavonoids with high-speed shearing assisted DESs was the best, the optimal extraction process was as follows: Speed 14000 r/min, material-to-liquid ratio 1:20 g/mL, extraction time 5 min, the average flavonoid extraction volume was 7.11 mg/g. The flavonoid content of Lycium barbarum from different origins in descending order was: Ningxia Yinchuan>Ningxia Zhongwei>Gansu Guazhou>Ningxia Guyuan>Xinjiang Jinghe>Ningxia Zhongning>Gansu Baiyin>Gansu Wuwei>Gansu Yumen>Xinjiang Altai>Xinjiang Yining>Qinghai Dulan>Qinghai Golmud>South Korea b>South Korea a. SIMCA 14.1 Software analysis showed that the positive regression coefficient value of Lycium barbarum flavonoids content and temperature and frost-free period was the largest, and the absolute value of negative regression coefficient with altitude was the largest. The most important ecological factors affecting the content of Lycium barbarum flavonoids were temperature and frost-free period.
- Lycium barbarum flavonoid extraction,
- deep eutectic solvent,
- high-speed shear,
- temperature,
- altitude,
- ecological factor

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