Determination of Amylose and Amylopectin Contents in Yam and Taros by Dual-Wavelength Spectrophotometry

HE Jie; YAN Feiyan; HUANG Fang; XIAO Yanni; XIE Liping

doi:10.13386/j.issn1002-0306.2021070119

Volume 43 Issue 7

Mar. 2022

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Science and Technology of Food Industry > 2022 > 43(7): 303-309. > DOI: 10.13386/j.issn1002-0306.2021070119

HE Jie, YAN Feiyan, HUANG Fang, et al. Determination of Amylose and Amylopectin Contents in Yam and Taros by Dual-Wavelength Spectrophotometry[J]. Science and Technology of Food Industry, 2022, 43(7): 303−309. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021070119.

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Determination of Amylose and Amylopectin Contents in Yam and Taros by Dual-Wavelength Spectrophotometry

Agro-products Quality Safety and Testing Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China

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Received Date: July 08, 2021
Available Online: February 10, 2022

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Abstract

Objective: The paper aims to establish a dual-wavelength spectrophotometry method for the determination of the content of amylose and amylopectin in yam and taros, and to provide basic data for variety selection, quality grading, product processing and standard formulation. Method: The spectrum of amylose and amylopectin were studied and the maximum absorption wavelength and reference wavelength were determined. The color conditions such as dispersant and acetic acid and measuring conditions were optimized. The absorbance values of the color complexes were determined. Result: The standard curve equation of amylose was y=0.0259x−0.0051, R²=0.9994, with the linear range of 0~33 μg/mL. The standard curve equation of amylopectin was y=0.0031x+0.0167, R²=0.9965, with the linear range of 0~111 μg/mL. The limits of detection and quantification of amylose were 0.093 μg/mL and 0.28 μg/mL respectively. The limits of detection and quantification of amylopectin were 0.079 μg/mL and 0.24 μg/mL, respectively. The recovery of amylose was 90.0%~100.0%. The recoveries of amylopectin were 88.0%~108.0%. Conclusion: The dual-wavelength spectrophotometry method was simple and feasible, accurate and reliable. It was suitable for the determination of amylose and amylopectin in yam and taros.
- dual-wavelength spectrophotometry,
- yam and taros,
- amylose,
- amylopectin

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