Optimization of Extraction Technique and Antioxidant Activity of Anthocyanins from <i>Rosa davurica</i> Pall.

ZHOU Xinyu; LV Chongning; QIN Rulan

doi:10.13386/j.issn1002-0306.2021060054

Volume 43 Issue 4

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(4): 178-186. > DOI: 10.13386/j.issn1002-0306.2021060054

ZHOU Xinyu, LV Chongning, QIN Rulan. Optimization of Extraction Technique and Antioxidant Activity of Anthocyanins from Rosa davurica Pall. DOI: 10.13386/j.issn1002-0306.2021060054

Citation:

ZHOU Xinyu, LV Chongning, QIN Rulan. Optimization of Extraction Technique and Antioxidant Activity of Anthocyanins from Rosa davurica Pall. DOI: 10.13386/j.issn1002-0306.2021060054

Citation:

ZHOU Xinyu, LV Chongning, QIN Rulan. Optimization of Extraction Technique and Antioxidant Activity of Anthocyanins from Rosa davurica Pall. DOI: 10.13386/j.issn1002-0306.2021060054

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Optimization of Extraction Technique and Antioxidant Activity of Anthocyanins from Rosa davurica Pall.

1.
School of Pharmacy and Medicine, Tonghua Normal University, Tonghua 134000, China
2.
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China

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Received Date: June 07, 2021
Available Online: December 15, 2021

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Abstract

Abstract

In order to optimize the extraction conditions of anthocyanins from Rosa davurica Pall. and investigate its antioxidant activity, single-factor experiment, Placket-Bumrman design, Box-Behnken analysis associated with ultrasonic extraction method were adopted. The antioxidant activity of anthocyanins from Rosa davurica Pall. were investigated by various methods, such as removal DPPH radical, ${\rm{O}}_2^-\cdot$ radical, ABTS⁺ racial. The results showed that the optimum extraction conditions were as follows: Solid-liquid ratio was 1:15 g·mL⁻¹, ultrasonic temperature was 50 ℃, ethanol volume was 60%, ultrasonic time was 30 min, pH was 2, and extraction times was 2. The content anthocyanins of Rosa davurica Pall. was 185.033 mg·100 g⁻¹. The antioxidant activity was measured with IC₅₀ and the results showed that the removal ability to DPPH free radical, superoxide anion free radical, ABTS⁺ free racial of the anthocyanins was 2.299, 1.902, 8.993 mg·mL⁻¹, respectively. The technology in the current study was easy and feasible. The content of anthocyanins was considerable. Furthermore, it showed that the anthocyanins had certain antioxidant activity in various antioxidant experiments.
- Rosa davurica Pall.,
- anthocyanins,
- Plackett-Burman design,
- response surface analysis,
- antioxidant activity

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References (33)

References

[1]	李子晗, 杨名春, 李庆鹏, 等. 模糊数学感官评价结合响应面法优化刺玫果苹果低糖复合果酱配方[J]. 中国调味品,2021,46(6):107−113. [LI Z H, YANG M C, LI Q P, et al. Optimization of low sugar compound jam formula of Rosa davurica Pall. and apple by fuzzy mathematical sensory evaluation and response surface method[J]. China Condiment,2021,46(6):107−113. doi: 10.3969/j.issn.1000-9973.2021.06.020
[2]	翟春梅, 怀雪, 孟祥瑛, 等. 基于果蝇模式生物差异基因表达的刺玫果抗衰老作用机理研究[J]. 天然产物研究与开发, 2020, 32(11): 1896−1903. ZHAI C M, HUAI X, MENG X Y, et al. Study on anti-aging mechanism of Rosa davurica Pall. based on differential gene expression in Drosophila model[J]. Natural Product Research and Development, 2020, 32(11): 1896−1903.
[3]	HWANG D H, LEE D Y, KOH P O, et al. Rosa davurica Pall. improves propionibacterium acnes-induced inflammatory responses in mouse ear edema model and suppresses pro-inflammatory chemokine production via mark and NF-κB pathways in hacaT cells[J]. International journal of molecular sciences,2020,21(5):1717−1733. doi: 10.3390/ijms21051717
[4]	邸松, 戴鹂莹, 钟方丽, 等. 双刺参胶囊总皂苷的提取、含量测定及体外活性[J]. 食品工业,2020,41(12):163−167. [DI S, DAI Y Y, ZHONG F L, et al. Extraction, content determination and in vitro activity of total saponins in Shuangcishen capsules[J]. The Food Industry,2020,41(12):163−167.
[5]	SHARMA N, TIWARI V, VATS S, et al. Evaluation of anthocyanin content, antioxidant potential and antimicrobial activity of black, purple and blue colored wheat flour and wheat-grass juice against common human pathogens[J]. Molecules,2020,25(24):5785. doi: 10.3390/molecules25245785
[6]	LIM S, XU J, KIM J, et al. Role of anthocyanin-enriched purple-fleshed sweet potato p40 in colorectal cancer prevention[J]. Molecular Nutrition & Food Research,2013,57(11):1908−1917.
[7]	吴映梅, 徐龙泉, 浦绍敏, 等. 黑莓中总花色苷的提取工艺优化及其抗氧化性和抑菌性的研究[J]. 食品研究与开发,2021,42(11):95−102. [WU Y M, XU L Q, PU S M, et al. Optimization of the extraction process of total anthocyanins from blackberries and the impact on the antioxidant and bacteriostatic activities[J]. Food Research and Development,2021,42(11):95−102. doi: 10.12161/j.issn.1005-6521.2021.11.016
[8]	闫亚美, 代彦满, 冉林武, 等. 黑果枸5种果蔬中花色苷组成及体外抗氧化活性比较[J]. 食品工业科技,2014,35(16):133−136. [YAN Y M, DAI Y M, RAN L W, et al. Composition and in vitro antioxidant activity of anthocyanins extracted from Lycium ruthenicum Murr., different fruits and vegetables[J]. Science and Technology of Food Industry,2014,35(16):133−136.
[9]	何传波, 米聪, 魏好程, 等. 紫薯花色苷的提取及抗氧化活性研究[J]. 热带作物学报,2016,37(5):990−997. [HE C B, MI C, WEI H C, et al. Extraction and antioxidant activity of anthocyanins from purple sweet potato[J]. Chinese Journal of Tropical Crops,2016,37(5):990−997. doi: 10.3969/j.issn.1000-2561.2016.05.022
[10]	周萍, 郑洁. 花色苷改性及应用研究进展[J]. 食品科学,2021,42(3):346−354. [ZHOU P, ZHENG J. Modification of anthocyanins for extended application: A review[J]. Food Science,2021,42(3):346−354. doi: 10.7506/spkx1002-6630-20200306-092
[11]	刘素稳. 蓝靛果花色苷提取物调节胆固醇代谢机理研究[D]. 沈阳: 沈阳农业大学, 2017. LIU S W. Study on the mechanism of Lonicera caerulea berry anthocyanin extracts regulating cholesterol metabolism[D]. Shenyang: Shenyang Agricultural University, 2017.
[12]	NICOLE B E, CHINN M S, TRUONG V D. Extraction of anthocyanins from industrial purple-fleshed sweet potatoes and enzymatic hydrolysis of residues for fermentable sugars[J]. Industrial Crops and Products,2010,32(3):613−620. doi: 10.1016/j.indcrop.2010.07.020
[13]	张鹏, 骆琴, 唐森, 等. pH示差法测定红花羊蹄甲花瓣花色苷的含量[J]. 食品工业,2020,41(6):303−306. [ZHANG P, LUO Q, TANG S, et al. Determining content of anthocyanins in petals of Bauhinia blakeana by pH differential method[J]. The Food Industry,2020,41(6):303−306.
[14]	CHEN R, LI S, LIU C, et al. Ultrasound complex enzymes assisted extraction and biochemical activities of polysaccharides from Epimedium leaves[J]. Proc Biochem,2012,47(12):2040−2050. doi: 10.1016/j.procbio.2012.07.022
[15]	程佑声, 王鸿飞, 许凤, 等. 蓝莓皮渣花色苷提取及抗氧化活性的研究[J]. 果树学报,2015,32(4):696−704. [CHENG Y S, WANG H F, XU F, et al. Study on extraction and antioxidant activity of anthocyanins from blue-berry pomace[J]. Journal of Fruit Science,2015,32(4):696−704.
[16]	WANG L, LIU H M, QIN G Y. Structure characterization and antioxidant activity of polysaccharides from Chinese quince seed meal[J]. Food chemistry,2017,234:314−322. doi: 10.1016/j.foodchem.2017.05.002
[17]	李国峰, 陈海芳, 杜文迪, 等. 超声辅助离子液体提取柚子皮总黄酮及其抗氧化研究[J]. 江西中医药大学学报,2021,33(1):84−88. [LI G F, CHEN H F, DU W D, et al. Ultrasonic assisted ionic liquid extraction of total flavonoids from pomelo peel and their antioxidant activities[J]. Journal of jiangxi university of TCM,2021,33(1):84−88.
[18]	冯娇, 肖海鸿. 不同产地银柴胡黄酮含量及其抗氧化活性研究[J]. 中国食品添加剂,2021,32(5):8−12. [FENG J, XIAO H H. Study on the flavonoids content and antioxidant activity of Stellaria dichotoma var. lanceolata cultivated from different areas[J]. China Food Additives,2021,32(5):8−12.
[19]	傅钰, 史璇, 张道明, 等. 低共熔溶剂提取马尾松松针抗氧化成分的研究[J]. 北京林业大学学报,2021,43(7):149−158. [FU Y, SHI X, ZHANG D M, et al. Antioxidant activities in extracts from Pinus massoniana needles by deep eutectic solvents[J]. Journal of Beijing Forestry University,2021,43(7):149−158. doi: 10.12171/j.1000-1522.20200030
[20]	CASTAÑEDA-OVANDO A, PACHECO-HERNÁNDEZ M D L, PÁEZ-HERNÁNDEZ M E, et al. Chemical studies of anthocyanins: A review[J]. Food Chemistry,2008,113(4):859−871.
[21]	AWIKA J M, ROONEY L W, WANISKA R D. Anthocyanins from black sorghum and their antioxidant properties[J]. Food Chemistry,2004,90(1/2):293−301.
[22]	ZOU T B, WANG M, GAN R Y, et al. Optimization of ultrasound-assisted extraction of anthocyanins from mulberry, using response surface methodology[J]. International Journal of Molecular Sciences,2011,12(5):3006−3017. doi: 10.3390/ijms12053006
[23]	万山, 杨喆, 朱保庆, 等. 响应面法优化红树莓花色苷超声辅助提取工艺的研究[J]. 食品工业科技,2016,37(3):220−224,228. [WAN S, YANG Z, ZHU B Q, et al. Parameters optimization of ultrasound-assisted extraction of anthocyanins from red raspberry by response surface methodology[J]. Science and Technology of Food Industry,2016,37(3):220−224,228.
[24]	ROMAN B, ANJA K, SHIFERAW T N, et al. Pressure and temperature effects on degradation kinetics and storage stability of total anthocyanins in blueberry juice[J]. Journal of Agricultural and Food Chemistry,2010,58:10076−10084. doi: 10.1021/jf1015347
[25]	蒋海伟, 杨婷婷, 李佳桥, 等. 响应面法优化红米花色苷微波辅助提取工艺及其抗氧化活性研究[J]. 中国食品学报,2015,15(5):74−81. [JIANG H W, YANG T T, LI J Q, et al. Response surface methodology for optimization of microwave-assisted extraction and antioxidant activity of anthocyanins from red rice[J]. Journal of Chinese Institute of Food Science and Technology,2015,15(5):74−81.
[26]	TAN J Y, LI Q, XUE H K, et al. Ultrasound-assisted enzymatic extraction of anthocyanins from grape skins: Optimization, identification, and antitumor activity[J]. Journal of Food Science,2020,85(11):3731−3744. doi: 10.1111/1750-3841.15497
[27]	ZHU B, LIU J L, GAO W D. Process optimization of ultrasound-assisted alcoholic-alkaline treatment for granular cold water swelling starches[J]. Ultrasonics Sonochemistry,2017,38:579−584. doi: 10.1016/j.ultsonch.2016.08.025
[28]	SAINI P, PARKASH S. Experimental investigation of machining parameters for surface, roughness in high speed CNC turning of EN-24 alloy steel, using response surface methodology[J]. International Journal of Engineering Research and Applications,2014,4(5):153−160.
[29]	JIANG H L, YANG J L, SHI Y P. Optimization of ultrasonic cell grinder extraction of anthocyanins from blueberry using response surface methodology[J]. Ultrasonics-Sonochemistry,2017,34:325−331. doi: 10.1016/j.ultsonch.2016.06.003
[30]	SHEN Y B, ZHANG H, CHENG L L, et al. In vitro and in vivo antioxidant activity of polyphenols extracted from black highland barley[J]. Food Chemistry,2016,194:1003−1012. doi: 10.1016/j.foodchem.2015.08.083
[31]	YONG P H, CHOI J H, YUN H J, et al. Anthocyanins from purple sweet potato attenuate dimethylnitrosamine-induced liver injury in rats by inducing Nrf2-mediated antioxidant enzymes and reducing COX-2 and iNOS expression[J]. Food & Chemical Toxicology,2011,49(1):93−99.
[32]	TAO H X, ZHOU J G, WU T, et al. High-throughput superoxide anion radical scavenging capacity assay[J]. Journal of Agricultural and Food Chemistry,2014,62(38):9266−9272. doi: 10.1021/jf502160d
[33]	PROTTI M, GUALANDI I, MANDRIOLI R, et al. Analytical profiling of selected antioxidants and total antioxidant capacity of goji (Lycium spp.) berries[J]. Journal of Pharmaceutical and Biomedical Analysis,2017,143:252−260. doi: 10.1016/j.jpba.2017.05.048

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