Optimization of Extraction Process of Buddleoside in Chrysanthemum indicum L. by Response Surface Methodology and Determination of Buddleoside Content in C. indicum L. from Different Regions in Dawu County

In order to explore the content of buddleoside in the Chrysanthemum indicum L. of Dawu County, Hubei Province, based on single factor experimental analysis, the Box-Behnken design was used to optimize the main factors affecting the yield of buddleoside in this study. And the content of buddleoside in C. indicum L. samples from different regions in Dawu County, Xiaogan City, Hubei Province was determined by using the optimum extraction process. The results showed that the solute mass fraction, liquid-solid ratio, extraction temperature and extraction time were the four main factors affecting the yield of buddleoside, and the extraction temperature had the most influence on the yield of buddleoside, followed by the extraction time, liquid-solid ratio and solute mass fraction. The optimum extraction conditions were as follows: mass fraction of ethanol was 54%, liquid-solid ratio was 150:1 (mL/g), extraction temperature was 80 ℃ and extraction time was 60 min. Under optimum conditions, the yield of buddleoside was 2.75%±0.03%, which was not significantly different from the predicted value of 2.80%. In addition, the conditions of the extraction process showed good stability (RSD<5%), high precision (RSD<5%), good recoveries (more than 80%), which was suitable for the determination of the content of buddleoside in the samples of C. indicum L.. The results showed that there were great differences in the content of buddleoside in C. indicum L. samples from different regions in Dawu County, ranging from 0 to 2.75%. Among them, samples 027, 037, 060 and 094 had higher content of buddleoside, all of which exceeded 2.5%. Our study can provide a reliable method for the quality evaluation of food materials and medicinal materials of C. indicum L., and could also provide an important basis for the cultivation and breeding of fine varieties of C. indicum L. with high content of buddleoside.