TANG Jianbo, LÜ Du, PENG Mei, et al. Optimization of Enzymatic-assisted Thermal Water Extraction of Rosa roxburghii Polysaccharide and Its Antitumor Activity[J]. Science and Technology of Food Industry, 2021, 42(18): 98−105. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030147.
Citation: TANG Jianbo, LÜ Du, PENG Mei, et al. Optimization of Enzymatic-assisted Thermal Water Extraction of Rosa roxburghii Polysaccharide and Its Antitumor Activity[J]. Science and Technology of Food Industry, 2021, 42(18): 98−105. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030147.

Optimization of Enzymatic-assisted Thermal Water Extraction of Rosa roxburghii Polysaccharide and Its Antitumor Activity

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  • Received Date: March 11, 2021
  • Available Online: July 14, 2021
  • In the present study, extraction process and antitumor activity of polysaccharide extracted from the Rosa roxbunghii Tratt (RRT) dried fruit were studied. The extraction technology of Rosa roxbunghii Tratt polysaccharide (RRTP) was optimized by mono-factor experiments and response surface methodology, and the antitumor activities of RRTP were studied by the model of S180 solid tumor. The results showed that the optimum extraction conditions for RRTP as follows: Enzymatic hydrolysis pH6.2, enzymatic hydrolysis time 4 h, enzymatic hydrolysis temperature 41 ℃, enzyme dosage 1.62%, liquid-solid ratio 30:1 mL/g, and then extracted at 90 ℃ hot water for 3 h after enzymatic hydrolysis completed. The yield of RRTP was 6.32%±0.12% on average under the optimum processing conditions. Furthermore, antitumor activity analysis showed that RRTP had obvious antitumor activity and could significantly (P<0.05) increase leukocyte count, thymus index and spleen index in S180 solid tumor mice. And the result indicated RRTP had a tumor inhibitory rate of 52.13%±1.84% in S180 solid tumor mice with gastric irrigation dose at 200 mg/kg. It was concluded that RRTP could improve the immune function and antitumor of S180 solid tumor mice, and could be used as a potential functional food supplements or natural substances of antitumor drugs.
  • [1]
    Xu J W, Vidyarthi S K, Bai W B, et al. Nutritional constituents, health benefits and processing of Rosa roxburghii: A review[J]. Journal of Functional Foods,2019,60:103456. doi: 10.1016/j.jff.2019.103456
    [2]
    Chen G J, Kan J Q. Characterization of a novel polysaccharide isolated from Rosa roxburghii Tratt fruit and assessment of its antioxidant in vitro and in vivo[J]. International Journal of Biological Macromolecules,2018,107:166−174. doi: 10.1016/j.ijbiomac.2017.08.160
    [3]
    周宏炫, 黄颖, 谭书明, 等. 刺梨多酚对急性酒精中毒大鼠的解酒护肝作用[J/OL]. 食品科学, 2020, doi: 10.7506/spkx1002-6630-20200 828-384.

    Zhou H X, Huang Y, Tan S M, et al. Anti-alcoholic and hepatoprotective effects of polyphenols from Rosa roxburghii Tratt in acute alcoholism rats[J/OL]. Food Science, 2020, doi: 10.7506/spkx1002-6630-20200 828-384.
    [4]
    夏仕青, 张爱华. 刺梨的营养保健功能及其开发利用研究进展[J]. 贵州医科大学学报,2018,43(10):1129−1132, 1153. [Xia S Q, Zhang A H. Research progress on nutrition and health function and exploitation of Rosa roxburghii Tratt[J]. Journal of Guizhou Medical University,2018,43(10):1129−1132, 1153.
    [5]
    杨宗玲, 李晗, 范方宇, 等. 超声辅助酶法提取无籽刺梨果渣中黄酮的工艺优化及其抗氧化活性[J/OL]. 食品工业科技, 2021, doi: 10.13386/j.issn1002-0306.2020090273.

    Yang Z L, Li H, Fan F Y, et al. Ultrasound-assisted enzymatic extraction of flavonoids from Rosa sterilis’pomace and its antioxidant[J/OL]. Science and Technology of Food Industry, 2021, doi: 10.13386/j.issn1002-0306.2020090273.
    [6]
    李跃红, 冉茂乾, 徐孟怀, 等. 不同产地刺梨果实品质分析与模糊综合评判[J]. 安徽农业科学,2020,48(17):202−205. [Li Y H, Ran M Q, Xu M H, et al. Analysis of fruit quality and fuzzy comprehensive evaluation ofRosa roxburghii Tratt from different habitats[J]. Journal of Anhui Agricultural Sciences,2020,48(17):202−205. doi: 10.3969/j.issn.0517-6611.2020.17.052
    [7]
    He J Y, Zhang Y H, Ma N, et al. Comparative analysis of multiple ingredients in Rosa roxburghii and R. sterilis fruits and their antioxidant activities[J]. Journal of Functional Foods,2016,27:29−41. doi: 10.1016/j.jff.2016.08.058
    [8]
    Chen Y, Liu Z J, Liu J, et al. Inhibition of metastasis and invasion of ovarian cancer cells by crude polysaccharides fromRosa roxburghii tratt in vitro[J]. Asian Pacific Journal of Cancer Prevention,2014,15(23):10351−10354.
    [9]
    Xu S J, Zhang F, Wang L J, et al. Flavonoids of Rosa roxburghii Tratt offers protection against radiation induced apoptosis and inflammation in mouse thymus[J]. Apoptosisi,2018,23:470−483. doi: 10.1007/s10495-018-1466-7
    [10]
    Xu P, Cai X H, Zhang W B, et al. Flavonoids ofRosa roxburghii Tratt exhibit radioprotection and anti-apoptosis properties via the Bcl-2(Ca2+)/Caspase-3/PARP-1 pathway[J]. Apoptpsis,2016,21:1125−1143. doi: 10.1007/s10495-016-1270-1
    [11]
    简崇东, 唐雄林, 黄晓华, 等. 脑梗死患者口服刺梨汁抗动脉粥样硬化临床研究[J]. 亚太传统医药,2017,13(3):136−137. [Jian C D, Tang X L, Huang X H, et al. Clinical research of anti-atherosclerosis by oral Rosa roxburghii Tratt juice in patients with acute cerebral infarction[J]. Asia-Pacific Traditional Medicine,2017,13(3):136−137.
    [12]
    涂永丽, 谭书明, 周宏炫, 等. 刺梨口服液对消化不良小鼠胃肠动力的促进作用[J]. 现代食品科技,2021,37(1):17−23. [Tu Y L, Tan S M, Zhou H X, et al. Effect of Rosa roxburghii Tratt oral liquid on gastrointestinal motility in dyspepsia mice[J]. Modern Food Science and Technology,2021,37(1):17−23.
    [13]
    邹胜, 徐溢, 张庆. 天然植物多糖分离纯化技术研究现状和进展[J]. 天然产物研究与开发,2015,27(8):1501−1509. [Zou S, Xu Y, Zhang Q. Review on extraction and purification technology of polysaccharides from natural plants[J]. Natural Product Research and Development,2015,27(8):1501−1509.
    [14]
    李秀秀, 尚静, 杨曦, 等. 多糖的增稠、胶凝及乳化特性研究进展[J/OL]. 食品科学, 2021, doi: 10.7506/spkx1002-6630-20200617-239.

    Li X X, Shang J, Yang X, et al. Rheology of food polysaccharides-thickening, gelling and emulsifying properties[J/OL]. Food Science, 2021, doi: 10.7506/spkx1002-6630-20200617-239.
    [15]
    唐健波, 肖雄, 杨娟, 等. 响应面优化超声辅助提取刺梨多糖工艺研究[J]. 天然产物研究与开发,2015,27(2):314−320. [Tang J B, Xiao X, Yang J, et al. Optimization of ultrasonic-assisted extraction of polysaccharides from Rosa roxburghii Tratt using response surface methodology[J]. Natural Product Research and Development,2015,27(2):314−320.
    [16]
    Zong S, Li J L, Ye Z Y, et al. Lachnum polysaccharide suppresses S180 sarcoma by boosting anti-tumor immune responses and skewing tumor-associated macrophages toward M1 phenotype[J]. International Journal of Biological Macromolecules,2020,144:1022−1033. doi: 10.1016/j.ijbiomac.2019.09.179
    [17]
    Cai B C, Pan J Y, Chen H, et al. Oyster polysaccharides ameliorate intestinal mucositis and improve metabolism in 5-fluorouracil- treated S180 tumour-bearing mice[J]. Carbohydrate Polymers,2021,256:117545. doi: 10.1016/j.carbpol.2020.117545
    [18]
    贾福怀, 涂宏建, 王俊, 等. 超声-闪式协同提取白及须根多糖工艺优化及其抗肿瘤活性[J]. 食品工业科技,2019,40(20):188−195, 208. [Jia F H, Tu H J, Wang J, et al. Optimization of ultrasonic-flash synergistic extraction and antitumor activity of polysaccharide from fibrous root of Bletilla striata[J]. Science and Technology of Food Industry,2019,40(20):188−195, 208.
    [19]
    姚佳, 彭梅, 肖雄, 等. 仙茅多糖对小鼠S180实体瘤的顺铂增敏作用及其机制[J]. 华西药学杂志,2014,29(2):132−134. [Yao J, Peng M, Xiao X, et al. Efficacy enhancing effect and mechanisms of Curculigo orchioides polysaccharide on S180 tumor-bearing mice treated by Cisplatin[J]. West China Journal of Pharmaceutical Sciences,2014,29(2):132−134.
    [20]
    高涛, 罗振宇, 罗黄洋, 等. 川明参多糖的超声辅助酶法提取及其体外抗氧化活性[J]. 食品研究与开发,2020,41(23):86−92. [Gao T, Luo Z Y, Luo H Y, et al. Ultrasound-assisted enzymatic extraction of polysaccharides from chuanminshen violaceum and its antioxidant acticity in virto[J]. Food Research and Development,2020,41(23):86−92.
    [21]
    Guo Y, Shang H M, Zhao J C, et al. Enzyme-assisted extraction of a cup plant (Silphium perfoliatum L.) polysaccharide and its antioxidant and hypoglycemic activities[J]. Process Biochemistry,2020,92:17−28. doi: 10.1016/j.procbio.2020.03.005
    [22]
    吕长鑫, 李萌萌, 徐晓明, 等. 响应面分析法优化纤维素酶提取紫苏多糖工艺[J]. 食品科学,2013,34(2):6−10. [Lü C X, Li M M, Xu X M, et al. Optimization of enzymatic extraction conditions for crude polysaccharides from perilla leaves by response surface methodology[J]. Food Science,2013,34(2):6−10.
    [23]
    Hu H G, Zhao Q L, Xie H J, et al. Polysaccharides from pineapple pomace: new insight into ultrasonic-cellulase synergistic extraction and hypoglycemic activities[J]. International Journal of Biological Macromolecules,2019,121:1213−1226. doi: 10.1016/j.ijbiomac.2018.10.054
    [24]
    Madondo M T, Quinn M, Plebanski M. Low dose cyclophosphamide: Mechanisms of T cell modulation[J]. Cancer Treatment Reviews,2016,42:3−9. doi: 10.1016/j.ctrv.2015.11.005
    [25]
    Strati P, Wierda W, Burger J, et al. Myelosuppression after frontline fludarabine, cyclophosphamide, and rituximab in patients with chronic lymphocytic leukemia: analysis of persistent and new-onset cytopenia[J]. Cancer,2013,119(21):3805−3811. doi: 10.1002/cncr.28318
    [26]
    Molodykh O P, Sorokina I V, Vinogradova E V, et al. Ultrastructure of the liver in response to cyclophosphamide and triterpenoids[J]. Bulletin of Experimental Biology and Medicine,2020,160:400−405.
    [27]
    Nevine R T, Amin H A, Sultan A A. The protective effect of Moringa oleifera leaves against cyclophosphamide-induced urinary bladder toxicity in rats[J]. Tissue and Cell,2015,47(1):94−104. doi: 10.1016/j.tice.2014.12.002
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