Study on Antioxidant Activity of Quercetin from Seabuckthorn Meal <i>in Vitro</i> and Its Protective Effect on Aging Mice

SHEN Xiaoxi; ZHANG Yiming; ZHAO Ziyi; SHI Chenhui; SHANG Hongli

doi:10.13386/j.issn1002-0306.2021020011

Volume 42 Issue 20

Oct. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(20): 348-354. > DOI: 10.13386/j.issn1002-0306.2021020011

SHEN Xiaoxi, ZHANG Yiming, ZHAO Ziyi, et al. Study on Antioxidant Activity of Quercetin from Seabuckthorn Meal in Vitro and Its Protective Effect on Aging Mice[J]. Science and Technology of Food Industry, 2021, 42(20): 348−354. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020011.

Citation:

PDF (2567 KB)

Study on Antioxidant Activity of Quercetin from Seabuckthorn Meal in Vitro and Its Protective Effect on Aging Mice

School of Food Science and Engineering, Jinzhou Medical University, Jinzhou 121001, China

More Information

Received Date: February 01, 2021
Available Online: August 19, 2021

Graphical Abstract

Abstract

Abstract

The antioxidant activity and protective effect of quercetin from seabuckthorn meal were studied in vitro. The antioxidant activities of quercetin in seabuckthorn were studied by 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH·) superoxide anion (${\rm{O}}_2^- \cdot $), 2, 2-diazo-bis (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS⁺·) and high iron ion reduction (FRAP). The aging model of mice was established by subcutaneous injection of D-galactose into the neck. The mice were treated with quercetin of seabuckthorn meal at different doses (100, 250, 500 mg/kg) by gavage. The eyeball blood was taken to measure the activities of total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and malondialdehyde (MDA) in serum; the heart, kidney and liver were taken as hematoxylin eosin staining (HE) to observe the pathological state of the cells. The results showed that quercetin from seabuckthorn meal had good scavenging ability on DPPH·, ${\rm{O}}_2^- \cdot $ and ABTS⁺·. Compared with the model group, the content of MDA in the low dose group of quercetin of seabuckthorn meal decreased significantly (P<0.05), the activities of T-AOC, GSH-Px and SOD in the serum of mice in the medium and high dose groups increased significantly (P<0.05), and the content of MDA decreased significantly (P<0.05). From the pathological section, with the increase of quercetin dosage in seabuckthorn meal, the myocardial fibers arranged orderly and the transverse lines were clear; the morphology of renal sacs and glomeruli gradually became normal, the number of renal tubules increased and arranged closely; the number of hepatocytes increased, the cell morphology was complete, and the sinus space gradually became significant. Therefore, quercetin from seabuckthorn meal could improve the activity of antioxidant enzymes and the content of reducing substances in aging model mice, reduce the content of lipid peroxide, so as to improve the antioxidant capacity of aging model mice, and had a better protective effect on aging model mice.
- seabuckthorn meal,
- quercetin,
- antioxidation in vitro,
- model mouse

FullText(HTML)

References (31)

References

[1]	焦岩, 常影, 余世锋, 等. 大果沙棘总黄酮体外抗氧化和抑菌作用研究[J]. 食品研究与开发,2015,36(19):12−15. [Jiao Yan, Chang Ying, Yu Shifeng, et al. In vitro antioxidant and antibacterial activities of total flavonoids from Hippophae rhamnoides[J]. Food Research and Development,2015,36(19):12−15. doi: 10.3969/j.issn.1005-6521.2015.19.004
[2]	张佳婵, 王昌涛, 赵丹, 等. 沙棘粕醇提物的定性定量分析及其对衰老小鼠肝脏抗氧化指标的影响[J]. 食品科学,2019,40(2):229−238. [Zhang Jiachan, Wang Changtao, Zhao Dan, et al. Qualitative and quantitative analysis of ethanol extract from seabuckthorn meal and its effect on liver antioxidant indexes of aging mice[J]. Food Science,2019,40(2):229−238. doi: 10.7506/spkx1002-6630-20180614-285
[3]	张佳婵, 王昌涛, 易思雨, 等. 沙棘粕提取物对H₂O₂诱导的B₁₆F₁₀细胞氧化损伤的保护及修复[J]. 中国食品学报,2019,19(8):13−21. [Zhang Jiachan, Wang Changtao, Yi Siyu, et al. Protection and repair of seabuckthorn meal extract on H₂O₂ induced oxidative damage in B16F10 cells[J]. Chinese Journal of Food,2019,19(8):13−21.
[4]	Rivera L, R Morón, M Sánchez, et al. Quercetin ameliorates metabolic syndrome and improves the inflammatory status in obese Zucker rats[J]. Obesity,2012,16(9):2081−2087.
[5]	游庭活, 温露, 刘凡. 衰老机制及延缓衰老活性物质研究进展[J]. 天然产物研究与开发,2015,27(11):1985−1990. [You Tinghuo, Wen LU, Liu Fan. Progress in the study of aging mechanism and anti-aging active substances[J]. Research and Development of Natural Products,2015,27(11):1985−1990.
[6]	Vetter J, P Novák, Wagner M R, et al. Ageing mechanisms in lithium-ion batteries[J]. Journal of Power Sources,2005,147(1-2):269−281. doi: 10.1016/j.jpowsour.2005.01.006
[7]	Jordi M, Czrina C, Gan W B, et al. Abnormal mitochondrial transport and morphology are common pathological denominators in SOD1 and TDP43 ALS mouse models[J]. Human Molecular Genetics,2014(6):1413−1424.
[8]	段亚云, 李建颖, 程瑶, 等. 黑果枸杞叶槲皮素的制备及其体外抗氧化活性研究[J]. 食品研究与开发,2019,40(5):59−64. [Duan Yayun, Li Jianying, Cheng Yao, et al. Preparation of quercetin from Lycium ruthenicum leaves and its antioxidant activity in vitro[J]. Food Research and Development,2019,40(5):59−64. doi: 10.3969/j.issn.1005-6521.2019.05.011
[9]	黄思莹, 黄春萍, 刘旭, 等. 桑叶槲皮素对D-半乳糖致衰老小鼠抗氧化能力及肠道菌群的影响[J]. 四川师范大学学报(自然科学版),2020,43(3):373−377. [Huang Siying, Huang Chunping, Liu Xu, et al. Effects of quercetin from mulberry leaves on antioxidant capacity and intestinal microflora in aging mice induced by D-galactose[J]. Journal of Sichuan Normal University (Natural Science Edition),2020,43(3):373−377.
[10]	Wang Y, Yu H, Wang S, et al. Targeted delivery of quercetin by nanoparticles based on chitosan sensitizing paclitaxel-resistant lung cancer cells to paclitaxel[J]. Materials Science and Engineering C,2020,119:111442.
[11]	吴春, 徐丽萍, 聂芊. 槲皮素-β-环糊精包合物作为亚硝化反应的抑制剂研究[J]. 食品与发酵工业,2007(7):78−80. [Wu Chun, Xu Liping, Nie Qian. Study on quercetin-β-cyclodextrin inclusion complex as an inhibitor of nitrosation[J]. Food and Fermentation Industry,2007(7):78−80.
[12]	Lu J, Papp L V, Fang J, et al. Inhibition of mammalian thioredoxin reductase by some flavonoids: Implications for myricetin and quercetin anticancer activity[J]. Cancer Research,2006,66(8):4410−4418. doi: 10.1158/0008-5472.CAN-05-3310
[13]	Zheng Yanyan, He Ruonan, Chen Zhongwei, et al. Optimization of extraction process of quercetin from flos sophorae immaturus using orthogonal design[J]. Medicinal Plant,2020,11(6):67−71.
[14]	童钰琴, 李姝, 牛曼思, 等. 苦荞麸皮总黄酮体外抗氧化活性及体内解酒护肝作用[J]. 食品工业科技,2020,41(17):314−319, 326. [Tong Yuqin, Li Shu, Niu Mansi, et al. Antioxidant activity of total flavonoids from buckwheat bran and its in vivo action of relieving wine and protecting liver[J]. Food Industry Technology,2020,41(17):314−319, 326.
[15]	孙丽萍, 王大仟, 张智武. 11种天然植物提取物对DPPH自由基的清除作用[J]. 食品科学,2009,30(1):45−47. [Sun Liping, Wang Daqian, Zhang Zhiwu. Scavenging effect of 11 natural plant extracts on DPPH free radical[J]. Food Science,2009,30(1):45−47. doi: 10.3321/j.issn:1002-6630.2009.01.010
[16]	Zhou G, Lv G. Comparative studies on scavenging DPPH free radicals activity of flavone C-glycosides from different parts of Dendrobium officinale[J]. China Journal of Chinese Materia Medica,2012,37(11):1536−1540.
[17]	胡月芳. 淮山多糖超声辅助提取及清除超氧阴离子自由基的作用[J]. 江苏农业科学,2017,45(4):144−146. [Hu Yuefang. Ultrasonic assisted extraction of polysaccharides from Huaishan mountain and its scavenging effect on superoxide anion radical[J]. Jiangsu Agricultural Sciences,2017,45(4):144−146.
[18]	Wu C Q, Chen G H. Detection of synergistic effect of superoxide dismutase and jujubosides on scavenging superoxide anion radical by capillary electrophoresis[J]. Biomedical Chromatography,2019,33(10):e4630.
[19]	孟庆焕, 王化, 王洪政, 等. 牡丹种皮黄酮提取及对ABTS自由基清除作用[J]. 植物研究,2013,33(4):504−507. [Meng Q H, Wang H, Wang H Z, et al. Extraction of flavonoids from peony seed coat and its scavenging effect on ABTS free radical[J]. Plant Research,2013,33(4):504−507. doi: 10.7525/j.issn.1673-5102.2013.04.020
[20]	Liu Z, Shi Y, Liu H, et al. Purification and identification of pine nut (Pinus yunnanensis Franch.) protein hydrolysate and its antioxidant activity in vitro and in vivo[J]. Chemistry & Biodiversity,2021,18:e2000710.
[21]	王蔚新, 张俊, 占剑峰, 等. 福白菊胎菊与朵菊粗多糖体外抗氧化活性分析[J]. 黄冈师范学院学报,2020,40(6):9−13. [Wang Weixin, Zhang Jun, Zhan Jianfeng, et al. In vitro antioxidant activity of crude polysaccharides from Chrysanthemum morifolium and Chrysanthemum morifolium[J]. Journal of Huanggang Normal University,2020,40(6):9−13. doi: 10.3969/j.issn.2096-7020.2020.06.02
[22]	毕凯媛, 李娜, 崔珊珊, 等. 红树莓花色苷对D-半乳糖衰老模型小鼠的保护作用[J]. 食品工业科技,2019,40(6):279−284. [Bi Kaiyuan, Li Na, Cui Shanshan, et al. The protective effect of anthocyanin of rubbingus on D-galactose aging model mice[J]. Food Industry Technology,2019,40(6):279−284.
[23]	王兵亚, 连秀仪, 杨琛擘, 等. 夏黑葡萄花青素抗氧化活性及对D-半乳糖衰老模型小鼠的保护作用[J]. 食品工业科技,2020,41(3):22−26, 32. [Wang Bingya, Lian Xiuyi, Yang Chenbo, et al. Antioxidant activity of anthocyanins from Xiahei grape and its protective effect on D-galactose aging model mice[J]. Food Industry Science and Technology,2020,41(3):22−26, 32.
[24]	孙玉军, 江昌俊, 任四海. 秀珍菇多糖对D-半乳糖致衰老小鼠的保护作用[J]. 食品科学,2017,38(5):251−256. [Sun Yujun, Jiang Changjun, Ren Sihai. Protective effect of Pleurotus ostreatus polysaccharide on aging mice induced by D-galactose[J]. Food Science,2017,38(5):251−256. doi: 10.7506/spkx1002-6630-201705041
[25]	石艳宾, 鲍佳彤, 王亦萱, 等. 金银花绿原酸与芦丁、槲皮素协同抗氧化作用[J]. 食品工业,2020,41(5):199−202. [Shi Yanbin, Bao Jiatong, Wang Yixuan, et al. Synergistic antioxidant effect of chlorogenic acid of honeysuckle with rutin and quercetin[J]. Food Industry,2020,41(5):199−202.
[26]	田建华, 张春媛, 魏璐. 沙棘果渣总黄酮提取工艺优化及抗氧化活性研究[J]. 天然产物研究与开发,2021,33(1):65−72. [Tian Jianhua, Zhang Chunyuan, Wei Lu. Optimization of extraction technology and antioxidant activity of total flavonoids from seabuckthorn fruit residue[J]. Research and Development of Natural Products,2021,33(1):65−72.
[27]	肖颖, 余英才, 余雄英, 等. 异鼠李素和槲皮素抗氧化活性研究[J]. 时珍国医国药,2012,23(5):1118−1120. [Xiao Ying, Yu Yingcai, Yu Xiongying, et al. Study on the antioxidant activities of isorhamnetin and quercetin[J]. Shizhen National Medicine,2012,23(5):1118−1120. doi: 10.3969/j.issn.1008-0805.2012.05.031
[28]	He M, Zhao L, Wei M J, et al. Neuroprotective effects of (-)-epigallocatechin-3-gallate on aging mice induced by D-galactose[J]. Biological & Pharmaceutical Bulletin,2009,32(1):55−60.
[29]	Pei L P, Dong F H. Effect of flavone of Pueraria lobata Ohwi on osseous changes in the aged rats induced by D-galactose[J]. Chinese Journal of Gerontology,2007:1750−1753.
[30]	张勍, 汪晟坤, 连秀仪, 等. 夏黑葡萄花青素在D-半乳糖致衰老小鼠心肌细胞抗氧化和清除自由基中的作用[J]. 河南医学研究,2019,28(20):3649−3652. [Zhang Qiong, Wang Shengkun, Lian Xiuyi, et al. Effect of anthocyanins from Xiahei grape on antioxidation and free radical scavenging of cardiomyocytes in aging mice induced by D-galactose[J]. Henan Medical Research,2019,28(20):3649−3652. doi: 10.3969/j.issn.1004-437X.2019.20.001
[31]	包晓玮, 李建瑛, 任薇, 等. 沙棘多糖对D半乳糖致衰老小鼠的抗氧化作用[J]. 食品工业科技,2020,41(4):293−297, 306. [Bao Xiaowei, Li Jianying, Ren Wei, et al. Antioxidant effect of seabuckthorn polysaccharide on aging mice induced by D-galactose[J]. Food Industry Science and Technology,2020,41(4):293−297, 306.

[1]	SHEN Chenglong, CHEN Yifan, ZENG Yingjie, CHEN Lianhong. Study on Antioxidant Activity of Tricholoma matsutake Polysaccharide in Vitro and Its Effect on Mice Damaged by Ethanol Oxidation[J]. Science and Technology of Food Industry, 2022, 43(23): 371-377. DOI: 10.13386/j.issn1002-0306.2022020248
[2]	HU Fenqing, LI Cuibai, DANG Lingjing, ZOU Cheng, ZHAO Qing, SHAO Yuefeng. Research Progress of Anti-lung Cancer Effect of Quercetin in Vitro[J]. Science and Technology of Food Industry, 2022, 43(18): 416-424. DOI: 10.13386/j.issn1002-0306.2021080017
[3]	ZHANG Weijie, XIE Xingfei, YAN He. Effect of a Compound Chinese Herbal Medicine on Cognitive Dysfunction and Gut Microbiota in the Model Mouse with Alzheimer’s Disease[J]. Science and Technology of Food Industry, 2021, 42(13): 345-350. DOI: 10.13386/j.issn1002-0306.2020080074
[4]	WANG Han, ZHU Hua-ping, LI Wen-zhao, RUAN Mei-juan. Analysis of Anthocyanin in Bilberry Extract and Its Antioxidant Activity in Vitro[J]. Science and Technology of Food Industry, 2019, 40(23): 60-65. DOI: 10.13386/j.issn1002-0306.2019.23.010
[5]	WANG Xiang, HU Feng-yang, YANG Qiu-ling, PENG Sheng, WANG Zhi-hong, ZHANG Ming-long, PENG Mi-jun. Nutritional Evaluation and Antioxidant Activity Analysis in Vitro of Eucommia Ulmoides Leaves[J]. Science and Technology of Food Industry, 2019, 40(21): 290-299. DOI: 10.13386/j.issn1002-0306.2019.21.048
[6]	ZHOU Xian-rong, ZHAO Xin, LONG Xing-yao, MU Jian-fei, PAN Yan-ni, QIAN Yu. Comparison of Antioxidative Effects of Polyphenols from Raw Puer Tea in Vitro and Their Protective Effects on Alcoholic Gastric Injury in Mice[J]. Science and Technology of Food Industry, 2019, 40(12): 300-308. DOI: 10.13386/j.issn1002-0306.2019.12.049
[7]	LI Bai-cun, LI Mu-ci, WANG Guo-liang, WANG Ke, LI Jian-ke. The antioxidant activity of pomegranate seed polyphenols in vitro[J]. Science and Technology of Food Industry, 2018, 39(4): 17-20.
[8]	ZHU Zhen-yuan, JIA Chang-ying, SUN Hui-qing. Study on compositions and antioxidant activities of fermented rice by Cordyceps militaris[J]. Science and Technology of Food Industry, 2016, (20): 235-238. DOI: 10.13386/j.issn1002-0306.2016.20.038
[9]	LIU Gong-cheng, WANG Zuo-wei, LI Ting-ting, DU Jin-ying, DU Xiao-gang, CAO Xiao-han, ZENG Xian-yin. Effect of polysaccharides from the leaves of Paris polyphylla on immune function and antioxidant capacities of mouse spleen tissue in a D-galactose-induced aging mouse model[J]. Science and Technology of Food Industry, 2015, (16): 366-369. DOI: 10.13386/j.issn1002-0306.2015.16.066
[10]	DAI Yi, CAO Dian-jie, XIA Lian-feng. Study on the antioxidant activities of Erzi Juhua Yin and the contents of luteolin and quercetin[J]. Science and Technology of Food Industry, 2015, (05): 282-285. DOI: 10.13386/j.issn1002-0306.2015.05.051

Supplements (0)

Cited By

Cited by

Periodical cited type(7)

1.	韩蓉，马燕，敖羽，张婷，孟新涛，许铭强，潘俨. 基于多元分析法综合评价新疆不同品种大果沙棘汁品质特性及加工适宜性. 食品工业科技. 2025(03): 322-332 . 本站查看
2.	屈凝伊，赵轶轩，梁丽，王丽娜. 沙棘果不同极性部位体外及体内抗氧化作用研究. 辽宁中医药大学学报. 2024(03): 13-17 .
3.	李纪彤，褚金国，丁丁. 沙棘多糖抗衰老的研究进展. 实用老年医学. 2024(03): 228-231 .
4.	于淼. 黑龙江省沙棘资源开发及其药理作用探究. 现代园艺. 2024(14): 122-124 .
5.	冯丹琦，王向红，吴迪，连运河，程鑫颖，刘卫华，米思. 基于RAW264.7细胞模型的槲皮万寿菊素与叶黄素协同改善急性肺损伤的作用机制. 食品科学. 2024(17): 96-104 .
6.	吴越，关磊，黎秋杞，杨婧娟，张希，冯凤琴，邵云东，程勇. 药食同源中药材抗衰老作用研究. 中国中医基础医学杂志. 2023(06): 1049-1055 .
7.	任李成城，刘振华，董琦，王洪伦，胡娜. 沙棘黄酮类成分及其药理作用的研究进展. 中国药物化学杂志. 2023(08): 598-617 .