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Volume 42 Issue 19
Sep.  2021
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YANG Zijing, RAO Guiwei, WANG Lei. Optimization of Ultrasonic Assisted Aqueous Two-phase Extraction of Total Flavonoid from Loquat Flower and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2021, 42(19): 218−225. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010186.
Citation: YANG Zijing, RAO Guiwei, WANG Lei. Optimization of Ultrasonic Assisted Aqueous Two-phase Extraction of Total Flavonoid from Loquat Flower and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2021, 42(19): 218−225. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010186.
shu

Optimization of Ultrasonic Assisted Aqueous Two-phase Extraction of Total Flavonoid from Loquat Flower and Its Antioxidant Activity

  • 1.

    College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China

  • 2.

    Hangzhou Normal University Qianjiang College, Hangzhou 310015, China

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  • Received Date: January 24, 2021
  • Available Online: August 01, 2021
    Graphical Abstract
    • Abstract
  • Ultrasonic assisted aqueous two-phase extraction of total flavonoid from loquat flower and its antioxidant activity were studied. With the yield of total flavonoid as the index, the extraction conditions were optimized by single factor test and response surface design, and the antioxidant effect was evaluated by measuring the damage of H2O2, the scavenging rate of ·OH and total reducing power.The results showed that the optimum extraction condition were determined as follows: The time of ultrasonic was 45 min, the mass content of loquat flower, (NH4)2SO4 and PEG400 was 0.2%, 18% and 28%. By adopting the process, the extraction yield of total flavonoid from loquat flower was 26.96%.The damage of H2O2 to total flavonoid was not obvious, the half scavenging concentration (IC50) values of ·OH was 0.297 mg/mL.The scavenging effect was superior to vitamin C (VC). The results suggest that total flavonoid from loquat flower is potential natural antioxidant.
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