Optimization of Ultrasonic Assisted Aqueous Two-phase Extraction of Total Flavonoid from Loquat Flower and Its Antioxidant Activity

YANG Zijing; RAO Guiwei; WANG Lei

doi:10.13386/j.issn1002-0306.2021010186

Volume 42 Issue 19

Sep. 2021

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Science and Technology of Food Industry > 2021 > 42(19): 218-225. > DOI: 10.13386/j.issn1002-0306.2021010186

YANG Zijing, RAO Guiwei, WANG Lei. Optimization of Ultrasonic Assisted Aqueous Two-phase Extraction of Total Flavonoid from Loquat Flower and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2021, 42(19): 218−225. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010186.

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Optimization of Ultrasonic Assisted Aqueous Two-phase Extraction of Total Flavonoid from Loquat Flower and Its Antioxidant Activity

1.
College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China
2.
Hangzhou Normal University Qianjiang College, Hangzhou 310015, China

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Received Date: January 24, 2021
Available Online: August 01, 2021

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Abstract

Abstract

Ultrasonic assisted aqueous two-phase extraction of total flavonoid from loquat flower and its antioxidant activity were studied. With the yield of total flavonoid as the index, the extraction conditions were optimized by single factor test and response surface design, and the antioxidant effect was evaluated by measuring the damage of H₂O₂, the scavenging rate of ·OH and total reducing power.The results showed that the optimum extraction condition were determined as follows: The time of ultrasonic was 45 min, the mass content of loquat flower, (NH₄)₂SO₄ and PEG400 was 0.2%, 18% and 28%. By adopting the process, the extraction yield of total flavonoid from loquat flower was 26.96%.The damage of H₂O₂ to total flavonoid was not obvious, the half scavenging concentration (IC₅₀) values of ·OH was 0.297 mg/mL.The scavenging effect was superior to vitamin C (V_C). The results suggest that total flavonoid from loquat flower is potential natural antioxidant.
- loquat flower,
- total flavonoid,
- response surface method,
- aqueous two-phase extraction,
- ultrasound,
- antioxidant

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References

[1]	苏文炳, 原远, 白昀鹭, 等. 枇杷萜类物质鉴定与三萜酸合成研究进展[J]. 园艺学报,2019,46(9):1727−1738. [Su W B, Yuan Y, Bai Y L, et al. Advances in terpenoid identification and triterpene acid biosynthesis in loquat[J]. Horticultural Plant Journal,2019,46(9):1727−1738.
[2]	卿樱露, 孔钰婷, 何丹, 等. 新食品原料——枇杷叶研发现状与展望[J]. 粮食科技与经济,2020,45(1):145−148. [Qing Y L, Kong Y T, He D, et al. The progressing and expectation of new resource food-loquat leaf[J]. Grain Science and Technology and Ecnomy,2020,45(1):145−148.
[3]	王翰华, 阮洪生, 陈云. 枇杷花化学成分及其药理作用研究进展[J]. 中成药,2019,41(12):2977−2981. [Wang H H, Ruan H S, Chen Y. Research progress on chemical constituents and pharmacological effects of loquat flowers[J]. Chinese Traditional Patent Medicine,2019,41(12):2977−2981. doi: 10.3969/j.issn.1001-1528.2019.12.030
[4]	刘丽丽, 刘玉垠, 王杰, 等. 枇杷功能成分及生物活性研究进展[J]. 食品科学,2020,41(5):306−314. [Liu L L, Liu Y Y, Wang J, et al. A review of functional components and bioactivities in loquat (Eriobotrya japonica lindl.)[J]. Chinese Food Science,2020,41(5):306−314. doi: 10.7506/spkx1002-6630-20190128-361
[5]	葛金芳, 李俊, 姚宏伟, 等. 枇杷叶三萜酸的抗炎作用[J]. 安徽医科大学学报,2007(2):174−178, 214. [Ge J F, Li J, Yao H W, et al. The anti-inflammatory effect of triterpene acids of loquat leaf[J]. Acta Universitatis Medicinalis Anhui,2007(2):174−178, 214. doi: 10.3969/j.issn.1000-1492.2007.02.016
[6]	Toshima A, Matsui T, Noguchi M, et al. Identification of α-glucosidase inhibitors from a new fermented tea obtained by tearolling processing of loquat (Eriobotyra japonica) and green tea leaves[J]. J Sci Food Agric,2010,90:1545−1550. doi: 10.1002/jsfa.3983
[7]	闫永芳, 孙钧, 孟天真, 等. 枇杷花研究及开发进展[J]. 食品工业科技,2011,32(12):544−546, 551. [Yan Y F, Sun J, Meng T Z, et al. Advances at the research on chemical composition and utilization of loquat flower[J]. Science and Technology of Food Industry,2011,32(12):544−546, 551.
[8]	李琪, 张宏, 黄春萍, 等. 枇杷花化学成分及抗菌抗肿瘤活性研究[J]. 西南农业学报,2014,27(2):739−742. [Li Q, Zhang H, Huang C P, et al. Studies on chemical constituents of flowers of Eriobotrya japonica l. and antibacterial, antineoplastic activity[J]. Southwest China Journal of Agricultural Sciences,2014,27(2):739−742.
[9]	Huang G L, Ma J J, Sui S Y, et al. Optimization of extraction of loquat flowers polyphenolics and its antioxidant and anti-polyphenol oxidase properties[J]. Bioengineered,2020,11(1):281−290. doi: 10.1080/21655979.2020.1735604
[10]	Wu S K, Zhang N, Shen X R, et al. Preparation of total flavonoids from loquat flower and its protective effect on acute alcohol-induced liver injury in mice[J]. Journal of Food and Drug Analysis,2015,23(1):136−143. doi: 10.1016/j.jfda.2014.07.001
[11]	黄桂丽, 吴牧容, 马佳佳, 等. 枇杷幼果总黄酮提取工艺优化及其抗氧化活性分析[J]. 食品安全质量检测学报,2020,11(10):3112−3118. [Huang G L, Wu M R, Ma J J, et al. Optimization of extraction process of total flavonoids from young loquat fruit and analysis of its antioxidant activity[J]. Journal of Food Safety & Quality,2020,11(10):3112−3118.
[12]	郭和卫. 枇杷的种植与管理技术[J]. 现代园艺,2017(18):28−29. [Guo H W. Planting and management techniques of loquat[J]. Xiandai Horticulture,2017(18):28−29.
[13]	刘玉明, 李珂娴, 何颖, 等. NaNO₂-Al(NO₃)₃-NaOH比色法测定枇杷花水提液中总黄酮含量[J]. 中华中医药学刊,2018,36(1):248−251. [Liu Y M, Li K X, He Y, et al. Research on determination of total flavonoids content in water extraction of flowers of Eriobotrya japonica (thunb.) lindl[J]. Chinese Archives of Traditional Chinese Medicine,2018,36(1):248−251.
[14]	黄琼, 谢向机. 超声波辅助提取枇杷花黄酮类化合物工艺的优化[J]. 食品工业,2012,33(1):8−12. [Huang Q, Xie X J. Optimization of ultrasound-assisted extraction flavonoids from flower of Eriobotrya japonica[J]. The Food Industry,2012,33(1):8−12.
[15]	郭欣, 谢三都. 酶解结合超声波提取枇杷花黄酮类物质工艺优化[J]. 漳州职业技术学院学报,2013,15(3):1−6. [Guo X, Xie S D. Optimization of extraction process for the flavonoids from loquat flowers by ultrasonic combined with hydrolysis[J]. Journal of Zhangzhou Institute of Technology,2013,15(3):1−6. doi: 10.3969/j.issn.1673-1417.2013.03.001
[16]	杨宗玲, 李晗, 范方宇, 等. 超声辅助酶法提取无籽刺梨果渣中黄酮的工艺优化及其抗氧化活性[J/OL]. 食品工业科技: 1−15[2021-01-17]. http://gfffg97249d11acbd4493sbpbw9owxx9wx6cvc.fgfy.zjsru.cwkeji.cn: 8081/10.13386/j.issn1002-0306.2020090273. Yang Z L, Li H, Fan F Y, et al. Ultrasound-assisted enzymatic extraction of flavonoids from Rosa sterilis’ pomace and its antioxidant activity[J/OL]. Science and Technology of Food Industry: 1−15[2021-01-17]. http://gfffg97249d11acbd4493sbpbw9owxx9wx6cvc.fgfy.zjsru.cwkeji.cn: 8081/10.13386/j.issn1002-0306.2020090273.
[17]	陈晶, 李琪, 黄春萍, 等. 枇杷花总黄酮、总三萜的大孔树脂制备工艺[J]. 食品科学,2015,36(18):58−63. [Chen J, Li Q, Huang C P, et al. Preparation of flavonoids and triterpenoids from flowers of Eriobotrya japonica (thunb.) lindl by macroporous resins[J]. Chinese Food Science,2015,36(18):58−63. doi: 10.7506/spkx1002-6630-201518010
[18]	Chong Kar Yeen, Brooks Marianne Su Ling. Effects of recycling on the aqueous two-phase extraction of bioactives from haskap leaves[J]. Separation and Purification Technology,2021,255:117755. doi: 10.1016/j.seppur.2020.117755
[19]	吴德智, 田昌义, 郑强, 等. 超声辅助双水相提取艾纳香总黄酮的工艺优化及抗氧化活性的研究[J]. 江苏农业科学,2017,45(15):153−156. [Wu D Z, Tian C Y, Zheng Q, et al. Study on the optimization of ultrasound-assisted two-phase aqueous two-phase extraction of total flavonoids and antioxidant activity[J]. Jiangsu Agricultural Sciences,2017,45(15):153−156.
[20]	张敏娜, 朱军, 王光辉, 等. 月季花总黄酮两种提取方法的比较研究[J]. 济宁医学院学报,2012,35(1):18−19, 23. [Zhang M N, Zhu J, Wang G H, et al. Comparing two kinds of methods for extraction of the total flavonoids from Rosa chinensis[J]. Journal of Jining Medical University,2012,35(1):18−19, 23. doi: 10.3969/j.issn.1000-9760.2012.01.005
[21]	谢田伟, 陈发河, 吴光斌. 响应面法优化枇杷花黄酮提取工艺研究[J]. 集美大学学报(自然科学版),2012,17(5):343−350. [Xie T W, Chen F H, Wu G B. Optimization of extraction technology of flavonoids from loquat flower by response surface methodology[J]. Journal of Jimei University (Natural Science),2012,17(5):343−350.
[22]	陈建中, 葛水莲, 昝立峰, 等. 响应面试验优化双水相萃取大吴风草总黄酮工艺及抑菌活性测定[J]. 食品科学,2015,36(24):57−62. [Chen J Z, Ge S L, Zan L F, et al. Optimization of aqueous two-phase extraction of total flavonoids from Farfugium joaponicum (L. f.) kitam by response surface methodology and antibacterial activity[J]. Food Science,2015,36(24):57−62. doi: 10.7506/spkx1002-6630-201524010
[23]	肖连冬, 王莹, 李慧星. 乙醇/磷酸氢二钾双水相体系提取洋葱黄酮工艺条件研究[J]. 食品研究与开发,2020,41(8):160−165. [Xiao L D, Wang Y, Li H X. Study on the extraction of flavone from onion by ethanol/ dipotassium hydrogen phosphate two-phase aqueous system[J]. Food Research and Development,2020,41(8):160−165. doi: 10.12161/j.issn.1005-6521.2020.08.026
[24]	王敏, 李波, 刘超, 等. 双水相萃取-LC-MS/MS同时测定环境水中11种抗生素[J]. 广州化工,2016,44(22):73−77. [Wang M, Li B, Liu C, et al. Simultaneous determination of 11 antibiotics in environmental water by ATPE-LC-MS/MS[J]. Guangzhou Chemical Industry,2016,44(22):73−77. doi: 10.3969/j.issn.1001-9677.2016.22.027
[25]	卢俊彩, 崔岩岩, 陈火林, 等. 丙酮-硫酸铵双水相体系分离纯化红花红色素[J]. 化学研究与应用,2007(6):679−682. [Lu J C, Cui Y Y, Chen H L, et al. Separation and purification of carthamine from Carthamus tinctorius by acetone-ammonium sulfate aqueous two-phase system[J]. Chemical Research and Application,2007(6):679−682. doi: 10.3969/j.issn.1004-1656.2007.06.024
[26]	霍清, 王强. 利用丙酮/磷酸氢二钾双水相体系分离纯化绿原酸的研究[J]. 食品科学,2008,29(11):234−236. [Huo Q, Wang Q. Separation and purification of carthamine from carthamus tinctorius by acetone-ammonium sulfate aqueous two-phase system[J]. Food Science,2008,29(11):234−236. doi: 10.3321/j.issn:1002-6630.2008.11.051
[27]	邵圣娟, 卫静莉. 聚乙二醇-硫酸铵双水相体系萃取松花粉中总黄酮的工艺研究[J]. 食品工业科技,2017,38(11):266−269, 275. [Shao S J, Wei J L. Study on the extraction of total flavonoids from pine pollen in PEG400/(NH₄)₂SO₄ aqueous two-phase system[J]. Science and Technology of Food Industry,2017,38(11):266−269, 275.
[28]	陈敏星. 乌龙茶做青过程色度值及叶绿体内活性氧代谢变化研究[D]. 福建: 福建农林大学, 2014. Chen M X. Study on the changes of chromtic values and active oxygen metabolism in green-making process of oolong tea[D]. Fujian: Fujian Agriculture and Forestry University, 2014.
[29]	陈红梅, 谢翎. 响应面法优化半枝莲黄酮提取工艺及体外抗氧化性分析[J]. 食品科学,2016,37(2):45−50. [Chen H M, Xie L. Optimization of extraction process for flavonoid from Soutellaria barbata by response surface methodology and evaluation of its antioxidant activity[J]. Food Science,2016,37(2):45−50. doi: 10.7506/spkx1002-6630-201602008
[30]	Oyaizu M. Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine[J]. Japanese J Nutr,1986,44(6):307−316. doi: 10.5264/eiyogakuzashi.44.307
[31]	吴德智, 郑强, 李安, 等. 缬草总黄酮超声辅助双水相提取工艺优化及抗氧化活性研究[J]. 食品与机械,2017,33(5):162−167. [Wu D Z, Zheng Q, Li A, et al. The study on the ultrasonic-assisted aqueous two-phase extraction on flavonoids of Valeriana officinalis l. and antioxidant activity[J]. Food & Machinery,2017,33(5):162−167.
[32]	欧阳文, 王家坚, 熊利芝. 聚乙二醇-硫酸铵双水相体系分离纯化黄花蒿黄酮的研究[J]. 食品工业科技,2014,35(15):196−199. [Ou Y W, Wang J J, Xiong L Z. Separation and purification of flavonoids from Artemisia annua l. by polyethylene glycol-ammonium sulfate aqueous two-phase system[J]. Science and Technology of Food Industry,2014,35(15):196−199.
[33]	郭杰, 陶蕾, 王吉鸿, 等. 响应面试验优化超声波辅助双水相提取蕨麻多糖[J/OL]. 食品与发酵工业: 1−12[2021-03-23]. http://gfybf97249d11acbd4493sb6vf9wpfkvqw65cx.fcaa.zjsru.cwkeji.cn: 8081/10.13995/j.cnki.11-1802/ts.025762. Guo J, Tao L, Wang J H, et al. Optimization of ultrasonic-assisted aqueous two-phase extraction of polysacchar-ides from Potentilla anserina L. using response surface methodology[J/OL]. Food and Fermentation Industries: 1−12[2021-03-23].
[34]	Yikmis Seydi. Optimization of Uruset apple vinegar production using response surface methodology for the enhanced extraction of bioactive substances[J]. Foods(Basel, Switzerland),2019,8(3):107.
[35]	Garcia-Vaquero Marco, Rajauria Gaurav, Tiwari Brijesh, et al. Extraction and yield optimisation of fucose, glucans and associated antioxidant activities from Laminaria digitata by applying response surface methodology to high intensity ultrasound-assisted extraction[J]. Marine Drugs,2018,16(8):257. doi: 10.3390/md16080257
[36]	郑美瑜, 陆胜民, 陈剑兵, 等. 枇杷花总黄酮的提取工艺优化[J]. 食品与发酵科技,2009,45(4):52−54. [Zheng M Y, Lu S M, Chen J B, et al. Study on extraction technology of flavonoids in loquat flowers[J]. Food and Fermentation Sciences & Technology,2009,45(4):52−54.