Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells

DU Yuemei; LIU Yunfan; QIN Fei; GAO Liping

doi:10.13386/j.issn1002-0306.2020120173

Volume 43 Issue 23

Nov. 2022

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Science and Technology of Food Industry > 2022 > 43(23): 347-352. > DOI: 10.13386/j.issn1002-0306.2020120173

DU Yuemei, LIU Yunfan, QIN Fei, et al. Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells[J]. Science and Technology of Food Industry, 2022, 43(23): 347−352. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120173.

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Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells

DU Yuemei^{1, 2},
LIU Yunfan^{1, 2},
QIN Fei^{1, 2, ,},
GAO Liping^{1, 2}

1.
College of Biochemical Engineering, Beijing Union University, Beijing 100023, China
2.
Beijing Municipal Key Laboratory of Biologically Active Substances and Functional Food, Beijing 100191, China

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Received Date: December 17, 2020
Available Online: October 08, 2022

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Abstract

Abstract

Objective: To explore the effect of fish oil on the activity of cis-dichlorodiamineplatinum (Ⅱ) (CDDP) against A549 human lung adenocarcinoma cells and its protective effect on HEK293 cells damaged by CDDP. Methods: A549 and HEK293 cells were cultured in vitro, and the cells were divided into control group, CDDP group, fish oil group, fish oil+CDDP group; the cells were pretreated with different concentrations of fish oil, and CDDP injury models of CDDP group and fish oil+CDDP group were established at the same time. Cell viability was detected by CCK-8 method, and the kits were used to measure malondialdehyde (MDA) content, direduced glutathione (GSH) content, and superoxide dismutase (SOD) activity in HEK293 cells. Results: Fish oil at a concentration of 4~16 mg/L could significantly inhibit the cytotoxicity of HEK293 cells caused by CDDP (P<0.05). Fish oil at a concentration of 4 mg/L had the best protective effect, and fish oil at this concentration could significantly inhibit CDDP (20 mg/L) decreased GSH content and increased MDA content (P<0.05). When the fish oil concentration was ≥32 mg/L, the inhibitory effect of CDDP on A549 cells was significantly enhanced (P<0.05). Conclusion: Within a certain concentration range, fish oil has a certain protective effect on CDDP-induced damage to HEK293 of human embryonic kidney cells. Low-concentration fish oil has no obvious effect on the inhibitory effect of CDDP-induced A549 cells, and high-concentration fish oil enhances the anti-cancer effect of CDDP.
- fish oil,
- cisplatin,
- nephrotoxicity,
- anti-oxidant,
- antitumor

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References

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