Construction of Fingerprint of Dancong Tea Using HS-SPME/GC-MS
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Graphical Abstract
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Abstract
The extraction conditions for volatile compounds of Dancong tea by headspace solid phase micro-extraction, including the extraction time, the temperature and the ratio of tea and water, were optimized. Each sample was detected and analyzed by gas chromatography-mass spectrometry (GC-MS) to establish the volatile substance fingerprint of Dancong tea. The results showed that the optimal extraction conditions were 0.75:5 g/mL tea-water ratio, and extraction was performed at 90 ℃ for 50 min. The volatile substances fingerprints of Dancong tea identified 20 common peaks and the similarity was above 0.9. The discriminant model had good accuracy for the classification of Dancong tea in different regions. The accuracy rate of its own verification method and the interactive verification method were 100% while the external verification method was 80%. The results can provide experimental basis for the headspace solid phase microextraction of Dancong tea and provide a theoretical reference for the identification of Dancong tea.
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