Optimization of Hydrothermal Acid Controlled Extraction of Luteolin from <i>Perilla </i>Stem by Response Surface Methodology

TANG Fujie; WANG Xingmin; HE Mengyang; LI Xin; ZHANG Jiale; LI Qiudong; GONG Xingxin; CHEN Hongqi

doi:10.13386/j.issn1002-0306.2020120154

Volume 42 Issue 17

Aug. 2021

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Science and Technology of Food Industry > 2021 > 42(17): 194-200. > DOI: 10.13386/j.issn1002-0306.2020120154

TANG Fujie, WANG Xingmin, HE Mengyang, et al. Optimization of Hydrothermal Acid Controlled Extraction of Luteolin from Perilla Stem by Response Surface Methodology[J]. Science and Technology of Food Industry, 2021, 42(17): 194−200. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120154.

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Optimization of Hydrothermal Acid Controlled Extraction of Luteolin from Perilla Stem by Response Surface Methodology

1.
College of Environment and Resources, Chongqing Technology and Business University, Chongqing 400067, China
2.
Chongqing Engineering Research Center for Processing, Storage and Transportation of Characterized Agro-Products, Chongqing 400067, China

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Received Date: December 17, 2020
Available Online: June 24, 2021

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Abstract

Abstract

In order to improve the resource utilization of Perilla stem, the hydrothermal acid control method was used to extract luteolin from Perilla stem. Taking the extraction amount of luteolin as the response value, response surface method was used to optimize the optimum extraction conditions. The phase structure of Perilla stem before or after hydrothermal acid control was analyzed by FTIR and SEM characterization.The results showed that under the optimal conditions of hydrothermal time of 120 min, hydrothermal temperature of 230 ℃, solid-liquid ratio of 1:20 g/mL and citric acid concentration of 1%, luteolin (901.049 ± 0.60) μg/g could be extracted from 2.0 g Perilla stem. The model was feasible. FTIR analysis showed that hydrothermal acid control could destroy the functional groups C−O−C and C=O in the lignocellulosic of Perilla stem. SEM showed that hydrothermal acid control could reduce the mass transfer resistance of active substances such as luteolin and facilitate the dissolution of luteolin.
- Perilla stem,
- luteolin,
- hydrothermal acid control method,
- response surface,
- process optimization

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