Composition Analysis and Inhibitory Effect against <i>α</i>-Amylase and <i>α</i>-Glucosidase of Acorn Kernel Extractions

LIANG Zongyao; WEI Yuanyuan; REN Weiwei; LI Minmeng; LIU Jing; DUAN Xuchang

doi:10.13386/j.issn1002-0306.2020120118

Volume 42 Issue 17

Aug. 2021

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Science and Technology of Food Industry > 2021 > 42(17): 47-55. > DOI: 10.13386/j.issn1002-0306.2020120118

LIANG Zongyao, WEI Yuanyuan, REN Weiwei, et al. Composition Analysis and Inhibitory Effect against α-Amylase and α-Glucosidase of Acorn Kernel Extractions[J]. Science and Technology of Food Industry, 2021, 42(17): 47−55. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120118.

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Composition Analysis and Inhibitory Effect against α-Amylase and α-Glucosidase of Acorn Kernel Extractions

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China

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Received Date: December 13, 2020
Available Online: July 04, 2021

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Abstract

In order to explore the component and inhibitory effects of extractions of acorn kernel on α-amylase and α-glucosidase, the ethyl acetate、N-butanol and water extractions of Quercus variabilis BI acorn kernelwere obtained by ultrasonic-assisted solvent extraction and separation. The composition of the extractions were analyzed by using UV spectroscopy, and high performance liquid chromatography (HPLC). The inhibitory effects of the extractions on α-amylase and α-glycosidase were investigated by using enzyme inhibition measurement, inhibition kinetics and fluorescence quenching. The results showed that three acorn kernel extractions were highly similar in chemical composition and were mainly tannin. The ethyl acetate extraction contained 9 different substances include epicatechin gallate, gallate and gallocatechin gallate and epigallocatechin gallate. N-butanol extraction contained 16 kinds of substances such as epicatechin gallate、gallocatechin and gallocatechin gallate.Water extraction contained 21 different substances include gallic acid and chlorogenic acid. The three extractions had obvious inhibitory effects on α-amylase and α-glycosidase, the half inhibitory concentrations (IC₅₀) of ethyl acetate, n-butanol, and water extractions on α-amylase were 1.047, 1.122 and 4.031 mg/mL respectively. The IC₅₀ values on α-glucosidase were 0.014, 0.028 and 0.037 mg/mL respectively. The inhibition of three extractions were mainly mixed and reversible, and the fluorescence quenching was mainly static quenching, and dynamic quenching was auxiliary.
- acorn kernel extractions,
- composition analysis,
- α-amylase,
- α-glycosidase,
- inhibition kinetics

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[1]	Ishida Y, Hirota T, Sato S, et al. Discriminative analysis of free and esterified gallic acids in acorn shells by thermochemolysis-gas chromatography/mass spectrometry in the presence of organic alkalis[J]. Journal of Analytical and Applied Pyrolysis,2015,116:114−119. doi: 10.1016/j.jaap.2015.09.019
[2]	Luo X, Bai R, Zhen D, et al. Response surface optimization of the enzyme-based ultrasound-assisted extraction of acorn tannins and their corrosion inhibition properties[J]. Industrial Crops and Products,2019,129:405−413. doi: 10.1016/j.indcrop.2018.12.029
[3]	Fernandes A, Sousa A, Mateus N, et al. Analysis of phenolic compounds in cork from Quercus suber L. by HPLC–DAD/ESI–MS[J]. Food Chemistry,2011,125(4):1398−1405. doi: 10.1016/j.foodchem.2010.10.016
[4]	Gezici S, Sekeroglu N. Neuroprotective potential and phytochemical composition of acorn fruits[J]. Industrial Crops and Products,2019,128:13−17. doi: 10.1016/j.indcrop.2018.10.082
[5]	薛文艳, 张文辉, 杨斌, 等. 麻栎橡子单宁脱除工艺优化及抗氧化性[J]. 食品科学,2017,38(10):242−250. [Xue Wenyan, Zhang Wenbin, Yang Bin, et al. Extraction optimization and antioxidant activity of tannins from Quercus acutissima acorns[J]. Food Science,2017,38(10):242−250. doi: 10.7506/spkx1002-6630-201710040
[6]	张玥, 谢文霁, 杨可心, 等. 我国橡子资源的开发利用[J]. 中国林副特产,2014(4):85−88. [Zhang Yue, Xie Wenji, Yang Kexin, et al. Development and utilization of acorn resources in our country[J]. Forest By-Product and Speciality in China,2014(4):85−88. doi: 10.3969/j.issn.1001-6902.2014.04.041
[7]	Xu J, Wang X, Yue J, et al. Polyphenols from acorn leaves (Quercus liaotungensis) protect pancreatic beta cells and their inhibitory activity against α-Glucosidase and protein tyrosine phosphatase 1B[J]. Molecules,2018,23:2167. doi: 10.3390/molecules23092167
[8]	Uddin G, Rauf A. Phytochemical screening, antimicrobial and antioxidant activities of aerial parts of Quercus robur L.[J]. Middle-East Journal of Medicinal Plants Research,2012,1(1):1−4.
[9]	Cantos E, Espin J- C, Lopez B C, et al. Phenolic compounds and fatty acids from acorns (Quercus spp.), the main dietary constituent of free-ranged Iberian pigs[J]. Journal of Agricultural and Food Chemistry,2003,51(21):6248−6255. doi: 10.1021/jf030216v
[10]	凌晨, 宋强, 刘玛丽. 2型糖尿病血糖-胰岛素调节系统得稳定性研究[J]. 信阳师范学院学报(自然科学版),2017,30(2):180−184. [Ling Chen, Song Qiang, Liu Mali. Studies on stability of the glucose-insulin regulation system for T2DM[J]. Journal of XINYANG Normal University,2017,30(2):180−184.
[11]	陈娟, 师彦平. 糖尿病相关的主要酶靶及其治疗药[J]. 中国新药杂志,2003,12(7):509−512. [Chen Juan, Shi Yanping. Target enzyme for diabetes and antidiabetic agents[J]. Chinese Journal of New Drugs,2003,12(7):509−512. doi: 10.3321/j.issn:1003-3734.2003.07.004
[12]	吕凤霞, 陆兆新. α-淀粉酶抑制剂的研究进展[J]. 食品科学,2002,23(3):152−155. [Lv Fengxia, Lu Zhaoxin. The research advance on α - amylases inhibitor[J]. Food Science,2002,23(3):152−155. doi: 10.3321/j.issn:1002-6630.2002.03.043
[13]	Sun L, Warren F, Gidley M, et al. Mechanism of binding interactions between young apple polyphenols and porcine pancreatic a-amylase[J]. Food Chemistry,2019,283(15):468−474.
[14]	李艳琴, 周凤超, 陕方, 等. 荞麦麸皮提取物对α-葡萄糖苷酶活性的影响[J]. 食品科学,2010,31(17):10−13. [Li Yanqin, Zhou Fengchao, Shaan Fang, et al. Effects of different solvent extracts from buckwheat bran on α-glucosidase activity[J]. Food Science,2010,31(17):10−13.
[15]	Wang J, Wu T, Fang L, et al. Anti-diabetic effect by walnut (Juglans mandshurica Maxim.)-derived peptide LPLLR through inhibiting α-glucosidase and α-amylase, and alleviating insulin resistance of hepatic HepG2 cells[J]. Journal of Functional Foods,2020,69:103944. doi: 10.1016/j.jff.2020.103944
[16]	Xu J, Cao J, Yue J, et al. New triterpenoids from acorns of Quercus liaotungensis and their inhibitory activity against α-glucosidase, α-amylase and protein-tyrosine phosphatase 1B[J]. Journal of Functional Foods,2018,41:232−239. doi: 10.1016/j.jff.2017.12.054
[17]	玄永浩, 金英善, 胡晓燕. 麻栎橡子抗氧化及抗糖尿病活性的试验研究[J]. 现代农业科技,2009,17:340−341. [Xuan Yonghao, Jin Shanying, Hu Xiaoyan. Primary study on antioxidant activities and antidiabetic properties of Quercus acutissima Caruth acorn[J]. Modern Agricultural Science and Technology,2009,17:340−341. doi: 10.3969/j.issn.1007-5739.2009.17.236
[18]	Custódio L, Patarra J, Alberício F, et al. Phenolic composition, antioxidant potential and in vitro inhibitory activity of leaves and acorns of Quercus suber on key enzymes relevant for hyperglycemia and Alzheimer's disease. Industrial Crops and Products, 2015, 64: 45−51.
[19]	侯盼盼. 橡子壳多酚的提取分离及功能性研究[D]. 咸阳: 西北农林科技大学, 2018: 21. Hou Panpan. Extraction and separation and function of acorn shell polyphenols[D]. Xianyang: Northwest A&F University, 2018: 21.
[20]	韩国民, 陈锋, 侯敏, 等. 葡萄酒中14种单体酚的高效液相色谱测定[J]. 食品科学,2011,32(2):180−183. [Han Minguo, Chen Feng, Hou Min, et al. Determination of 14 mono-phenols in dry red wines by HPLC[J]. Food Science,2011,32(2):180−183.
[21]	张红城, 孙庆申, 王光新, 等. 蜂胶乙醇提取物对α-葡萄糖苷酶的抑制作用[J]. 食品科学,2011,32(5):108−110. [Zhang Hongcheng, Sun Qingshen, Wang Guangxin, et al. Inhibitory effect of ethanol extract from propolis on α-glucosidase[J]. Food Science,2011,32(5):108−110.
[22]	赵瑜, 周家春, 张靖伟, 等. 紫娟茶提取物对血管紧张素转换酶、α-淀粉酶和胰脂肪酶的体外抑制作用[J]. 食品工业科技,2017,38(19):11−20. [Zhao Yu, Zhou Jiachun, Zhang Jingwei, et al. Inhibitory effects of Zijuan tea (Camellia sinensis var. kitamura) extracts on angiotensin converting enzyme, α-amylase and pancreatic lipase in vitro[J]. Science and Technology of Food Industry,2017,38(19):11−20.
[23]	Han L, Peng Q, Zhu R. Inhibitory effect of phloretin on alpha-glucosidase: Kinetics, interaction mechanism and molecular docking[J]. International Journal of Biological Macromolecules,2017,95:520−527. doi: 10.1016/j.ijbiomac.2016.11.089
[24]	耿升. 显齿蛇葡萄叶多酚的α-葡萄糖苷酶抑制及抗氧化活性研究[D]. 新乡: 河南科技学院, 2017: 37. Geng Sheng. α-Glucosidase inhibitory and antioxidant activities of polyphenols in Ampelopsis grossedentata leaves[D]. Xinxiang: Henan Institute of Science and Technology, 2017: 37.
[25]	Ding H, Hu X, Xu X, et al. Inhibitory mechanism of two allosteric inhibitors, oleanolic acid and ursolic acid on alpha-glucosidase[J]. International Journal of Biological Macromolecules,2018,107(Part B):1844−1855.
[26]	樊美慧. 黄酮类化合物对酪氨酸酶的抑制机制及其构效关系研究[D]. 南昌: 南昌大学, 2018: 15. Fan Meihui. Study on the inhibition mechanism of flavonoids towards tyrosinase and structure-activity relationship[D]. Nanchang: Nanchang University, 2018: 15.
[27]	龚凌霄, 曹文燕, 张英, 等. 青稞麸皮提取物抑制α-葡萄糖苷酶活性研究及成分分析[J]. 食品科学,2017,38(6):179−184. [Gong Lingxiao, CaoWenyan, Zhang Ying, et al. Anti-α-glucosidase activities and bioactive components of Tibetan Hull-Less barley bran extracts[J]. Food Science,2017,38(6):179−184. doi: 10.7506/spkx1002-6630-201706028
[28]	高锦明. 植物化学[M]. 北京: 科学出版社, 2003: 176−178, 208−212. Gao Jinming. Phytochemistry[M]. Beijing: Science Press, 2003: 176−178, 208−212.
[29]	Cao J, Zhang Y, Han L, et al. Number of galloyl moieties and molecular flexibility are both important in alpha-amylase inhibition by galloyl-based polyphenols[J]. Food and Function,2020,11:3838−3850. doi: 10.1039/C9FO02735A
[30]	郭时印, 李林, 周虹, 等. 1-脱氧野尻霉素对α-葡萄糖苷酶的抑制作用机制[J]. 食品科学,2019,40(5):45−50. [Guo Shiyin, Li Lin, Zhou Hong, et al. Mechanism for the inhibitory effect of 1-deoxynojirimycin on α-glucosidase[J]. Food Science,2019,40(5):45−50. doi: 10.7506/spkx1002-6630-20180808-081
[31]	杨丽珍, 邹波, 徐玉娟, 等. 荔枝壳多酚对α-葡萄糖苷酶的抑制作用[J]. 食品科技,2017,42(5):174−179. [Yang Lizhen, Zhou Bo, Xu Yujuan, et al. Inhibitory effect of free and bound phenolics from litchi pericarp on α-glycosidase[J]. Food Science and Technology,2017,42(5):174−179.
[32]	姚芬. 柿单宁对淀粉消化特性的影响及其机制研究[D]. 武汉: 华中农业大学, 2017: 31−33. Yao Fen. Study on the effects of persimmon tannin on starch digestibility and its mechanism[D]. Wuhan: HuaZhong Agricultural University, 2017: 31−33.

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