Determination of 23 Sulfonamide Drugs Residues in Chicken by Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry with Pass-through Solid Phase Extraction

YANG Kun; LIU Guiqiong; GUAN Chuncheng; CHEN Guangjing

doi:10.13386/j.issn1002-0306.2020120074

Volume 42 Issue 18

Sep. 2021

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Science and Technology of Food Industry > 2021 > 42(18): 284-291. > DOI: 10.13386/j.issn1002-0306.2020120074

YANG Kun, LIU Guiqiong, GUAN Chuncheng, et al. Determination of 23 Sulfonamide Drugs Residues in Chicken by Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry with Pass-through Solid Phase Extraction[J]. Science and Technology of Food Industry, 2021, 42(18): 284−291. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120074.

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Determination of 23 Sulfonamide Drugs Residues in Chicken by Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry with Pass-through Solid Phase Extraction

1.
Qiandongnan Testing Center of Food and Drug Control, Kaili 556012, China
2.
Food and Pharmaceutical Engineering Institute, Guiyang University, Guiyang 550005, China

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Received Date: December 08, 2020
Available Online: July 12, 2021

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Abstract

Abstract

An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for simultaneous determination of 23 sulfonamide drugs residues in chicken. The samples were extracted by 80% acetonitrile solution (containing 0.2% formic acid) and the extracts were purified on Oasis PRiME HLB solid-phase extraction column. The 23 sulfonamide drugs were analyzed by Waters Acquity UPLC CSH C18 column (100 mm×2.1 mm, 1.7 μm) using methanol and 0.1% formic acid as the mobile phases, electrospray source positive ion scanning and retention time-dependent multiple reaction monitoring mode (Scheduled MRM) detection, external standard method for quantification. The results showed that there was a good linear relationship between 23 sulfonamide drugs in the linear range of 0.2~20 ng/mL, with the correlation coefficients all greater than 0.9996. The detection limits were 0.1~2.0 μg/kg, and the quantification limits were 0.25~5.0 μg/kg. The average recovery rates were 66.12%~99.83% and the relative standard deviations (RSD, n=6) form 0.72% to 10.36% at the three standard levels of 1.0, 2.0 and 5.0 μg/kg. The method is simple, sensitive and reproducible. The method is successfully used for accurate and quantitative measurement of multiple sulfanilamide drugs residues in chicken.

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