Study on Fungistasis of Marine Bacteria BMF04 and Removal Effect on Toxin

In order to clarify the inhibitory effects, toxin removal capacity and toxin degradation mechanism of marine bacteria BMF04. Firstly, the inhibitory effects of BMF04 strain and its aseptic fermentation broth on Fusarium graminearum and Fusarium nivale (Fr) Ces. were determined by the plate-stand method; Secondly, the removal of ZEN by live strain, aseptic fermentation broth, cell wall suspension and intracellular fluid, as well as the effect of hightemperature and protease treatment on the removal of ZEN by BMF04 strain were determined by toxicity plate method and HPLC method, to clarify the mechanism of its action of removing toxin. The results showed that BMF04 had good inhibitory effect on F. graminearum and F. nivale (Fr) Ces.. The strain BMF04 strain had a strong ability to remove Zen toxin, and when the concentration was 15 µg/mL, the live strain, the aseptic fermentation liquid, the cell wall suspension and the intracellular liquid had strong removal effect on ZEN, the removal rates were 98.92%±0.07%, 98.70%±0.19%, 97.85%±0.07% and 98.54%±0.10%, respectively. After high temperature inactivation, the removal rate of ZEN was reduced to 60.32%±0.21% and 2.09%±1.15%. After treatment with trypsin, pepsin and proteinase K, the removal rates decreased to 3.52%±0.77%, 0.50%±0.39% and 0.18%± 0.12%, indicating that high temperature inactivation and protease had significant effects on the removal of ZEN from BMF04 fermentation broth. Therefore, the removal of ZEN by the strain had both the degradation of extracellular protein and the adsorption of cell wall. The results of this study provided a new strain resource for the biological removal of ZEN toxin from contaminated food and feed, and also provided a theoretical basis for the biodegradation of ZEN toxin.