Study on the Preparation of Polysaccharide Lozenge from Morchella esculenta and Its Antioxidant Activity
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Graphical Abstract
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Abstract
With the amniotic bacteria sub-entity as the main raw material, by cellulase, papaya protease complex enzyme solution, ultrasound assisted extraction to obtain Morchella esculenta polysaccharides, further through the addition of auxiliary ingredients to develop amniotic bacteria polysaccharide tablets. Single-factor and response surface experimental optimization obtained the best conditions for the optimal extraction of Morchella esculenta polysaccharides for liquid ratio of 29:1 mL/g, enzymatic pH5.1, enzymatic time 2 h, enzyme dosage 1.4%, ultrasonic time 20 min, the rate of extraction reached up to 9.21%; Single-factor experiments and orthogonal experiments determined that the best type of filler for polysaccharide tablets of amniotic bacteria was glycol:sorbitol: 1:1, the composition of the ingredients as fillers and polysaccharide leaching paste ratio was 8:1, vitamin C was added 5 mg/g, the addition of stingric acid magnesium was 0.8%, and the comprehensive score of the tablets was 86.4 under this condition. When the tablet concentration was 10 mg/mL, the antioxidant activity results were: hydroxy radical clearance of 46.78% and DPPH radical clearance of 75.38%, with good antioxidant activity.
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