Effect of Tea Tree Oil on Methicillin-resistant <i>Staphylococcus aureus</i> Biofilm

CHENG Feng; LIU Yu; YANG Zhen; MO Yanan; SHANG Ruofeng; HAO Baocheng; WANG Xuehong; LIANG Jianping

doi:10.13386/j.issn1002-0306.2020110063

Volume 42 Issue 14

Jul. 2021

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Science and Technology of Food Industry > 2021 > 42(14): 107-112. > DOI: 10.13386/j.issn1002-0306.2020110063

CHENG Feng, LIU Yu, YANG Zhen, et al. Effect of Tea Tree Oil on Methicillin-resistant Staphylococcus aureus Biofilm[J]. Science and Technology of Food Industry, 2021, 42(14): 107−112. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020110063.

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Effect of Tea Tree Oil on Methicillin-resistant Staphylococcus aureus Biofilm

Key Laboratory of New Animal Drug Project, Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences, Lanzhou 730050, China

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Received Date: November 08, 2020
Available Online: May 18, 2021

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Abstract

Abstract

The effects of tea tree oil on the biofilm of Methicillin-resistant Staphylococcus aureus (MRSA) and its mechanism of action were studied in this paper . Firstly, the MIC values of tea tree oil against MRSA and 12 clinically isolated strains were determined to be 0.08%~0.32%. It was found that 0.16% of tea tree oil had an inhibitory effect on the formation of MRSA biofilms. Tea tree oil at 0.32% could completely remove mature biofilms of MRSA while 0.16% could significantly reduce the mature biofilms by semi-quantitative crystal violet experiments and CLSM. Preliminary exploration of the biofilm mechanism found that tea tree oil could inhibit the secretion of polysaccharide intercellular adhesion (PIA) and extracellular DNA (eDNA), and the expression level of agr, ica, cid and sar during the formation of MRSA biofilm. Therefore, tea tree essential oil would have a strong anti-biofilm effect, and could inhibite the synthesis of PIA, the secretion of eDNA and the expression of genes related to biofilm formation.
- tea tree oil,
- biofilm,
- semi-quantitative crystal violet experiments,
- CLSM,
- PIA,
- eDNA

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References

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