Determination of Cadmium in Water Samples by Nano-SiO<sub>2</sub> Preconcentration with hicELISA

ZHOU Ye; YANG Shuyu; GAO Yilin; CHEN Junjie; LIU Changzhong; WANG Ziliang; JIANG Jinqing

doi:10.13386/j.issn1002-0306.2020100133

Volume 42 Issue 14

Jul. 2021

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Science and Technology of Food Industry > 2021 > 42(14): 278-283. > DOI: 10.13386/j.issn1002-0306.2020100133

ZHOU Ye, YANG Shuyu, GAO Yilin, et al. Determination of Cadmium in Water Samples by Nano-SiO₂ Preconcentration with hicELISA [J]. Science and Technology of Food Industry, 2021, 42(14): 278−283. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020100133.

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Determination of Cadmium in Water Samples by Nano-SiO₂ Preconcentration with hicELISA

College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, China

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Received Date: October 19, 2020
Available Online: May 18, 2021

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Abstract

Objective: Explore the conditions of preconcentration and separation of Cd²⁺ in water samples by nano-SiO₂, prepare monoclonal antibody against Cd²⁺ and establish a rapid detection method of trace Cd²⁺ in water samples by heterologous indirect competitive ELISA (hicELISA). Methods: Cd²⁺ was preconcentrated by nano-SiO₂ and eluted by EDTA-2Na. The content of Cd²⁺ was determined by ICP. Balb/C mice were immunized with Cd-ITCBE-BSA to prepare Cd²⁺ monoclonal antibody. The heterologous coating antigen was synthesized to establish the detection method of cadmium ion hicELISA. Results: The adsorption capacity of Nano-SiO₂for Cd²⁺ was 13.3 mg/g, and the enrichment multiple was 20 times. EDTA could be used as the effective eluent for the pretreatment. Using Cd-ITCBE-BSA as immunogen, the titer of serum was 1:1.28×10⁴, The titer of mAb in ascites was 1:2.0×10⁵ and the affinity constantwas 8.1×10⁸ L/mol. The detection limit of hicELISA was 2.9 μg/L. There was no significant cross reaction with Hg²⁺, Cu²⁺, Zn²⁺, Pb²⁺, Cr³⁺, Mo⁶⁺, Fe³⁺, Co²⁺. The recoveries of cadmium in lake water, tap water and ultrapure water were 92.47%~102.86%. Conclusion: The hicELISA method was sensitive, specific and accurate, and could be used for the detection of cadmium in water samples.
- nano-SiO₂,
- heavy metal,
- cadmium ion,
- hicELISA,
- heterologous detection

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