Simultaneous Determination of Four Alkaloids in Betel Nut Seeds by Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

GUO Xiaojie; WANG Pan; PANG Zhaohai; HAN Bingjun; YANG Xinfeng

doi:10.13386/j.issn1002-0306.2020090152

Volume 42 Issue 14

Jul. 2021

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Science and Technology of Food Industry > 2021 > 42(14): 257-263. > DOI: 10.13386/j.issn1002-0306.2020090152

GUO Xiaojie, WANG Pan, PANG Zhaohai, et al. Simultaneous Determination of Four Alkaloids in Betel Nut Seeds by Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry [J]. Science and Technology of Food Industry, 2021, 42(14): 257−263. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020090152.

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Simultaneous Determination of Four Alkaloids in Betel Nut Seeds by Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

GUO Xiaojie^{1, 2,},
WANG Pan^{1, 3},
PANG Zhaohai^{2, 4},
HAN Bingjun^{2, 4},
YANG Xinfeng^{2, 4, ,}

1.
College of Tropical Crops, Hainan University, Haikou 570228, China
2.
Analysis and Testing Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
3.
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 570000, China
4.
Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Haikou 571101, China

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Received Date: September 15, 2020
Available Online: May 16, 2021

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Abstract

Abstract

Objective: A method was developed for the determination of arecoline, arecaidine, guvacoline and guvacine hydrochloride in areca nut seeds by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Methods: The sample were extracted with 45% ethanol aqueous solution, and detected by UPLC-MS/MS method. Chromatographic conditions: Separated by Waters Atlantis T3 (2.1 mm×150 mm, 5 μm) column, eluted by gradient with 0.1% formic acid aqueous solution-acetonitrile solution as mobile phase, with flow rate of 0.5 mL·min⁻¹, column temperature of 40 ℃, injection volume of 1 μL. Mass spectrometry conditions: Using electrospray ion source (ESI⁺), detection method multiple reaction monitoring (MRM) mode scanning, the quantitative analysis by external standard method. Results: In the range of 50~500 ng/mL, the mass concentration and peak area of arecoline, arecaidine, guvacoline and guvacine hydrochloride showed a good linear relationship (R²>0.99), and the sample recovery rates were all within the specified range of 70%~120%, the average recovery rate was 75.27%~96.70%, and the RSD was less than 7%. Conclusion: The method was good repeatability, stability, and instrument precision.

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Simultaneous Determination of Four Alkaloids in Betel Nut Seeds by Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

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