Determination of Zeaxanthin Dipalmitate and 2-O-(<i>β</i>-D-glucopyranosyl) Ascorbic Acid in Fruit of <i>Lycium barbarum </i>L.

ZHOU Huiji; PENG Bo; LI Tingzhao; CHEN Liang; LI Bo

doi:10.13386/j.issn1002-0306.2020090069

Volume 42 Issue 12

Jun. 2021

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Science and Technology of Food Industry > 2021 > 42(12): 294-299. > DOI: 10.13386/j.issn1002-0306.2020090069

ZHOU Huiji, PENG Bo, LI Tingzhao, et al. Determination of Zeaxanthin Dipalmitate and 2-O-( β -D-glucopyranosyl) Ascorbic Acid in Fruit of Lycium barbarum L. DOI: 10.13386/j.issn1002-0306.2020090069

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Determination of Zeaxanthin Dipalmitate and 2-O-(β-D-glucopyranosyl) Ascorbic Acid in Fruit of Lycium barbarum L.

ZHOU Huiji^1,,
PENG Bo²,
LI Tingzhao^{1, 2},
CHEN Liang¹,
LI Bo^{1, 2, ,}

1.
Amway (China) R&D Center, Shanghai 201203, China
2.
Amway (China) Botanical R&D Center, Wuxi 214145, China

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Received Date: September 15, 2020
Available Online: April 19, 2021

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Abstract

Abstract

Objective: To establish LC methods for zeaxanthin dipalmitate and 2-O-(β-D-glucopyranosyl) ascorbic acid determination in the fruit of Lycium barbarum L. Methods: For the HPLC analysis of zeaxanthin dipalmitate, the test was performed on a Luna C₁₈ (2) column (4.6 mm×250 mm, 5 μm) with the column temperature at 25 ℃. The isocratic elution was adopted with the mobile phase of acetone/MeOH 55:45(V/V) at a flow rate of 1.0 mL·min⁻¹, and UV detection was performed at 450 nm. UPLC analysis of ascorbic acid was carried out on Torus TM Diol Column (3 mm×100 mm, 1.7 μm) at the temperature of 40 ℃. An isocratic system was also used at which the mobile phase (flow rate of 0.4 mL/min) consisting of acetonitrile (solventA) and 66.7 mmol/L ammonium acetate (solvent B) was set as 85% A and 15% B. UV detection wavelength was set at 260 nm. Results: The linear range of zeaxanthin dipalmitate and 2-O-(β-D-glucopyranosyl) ascorbic acid were 0.01~0.1 mg/mL and 3.125~100 μg/mL, with the correlation coefficient being 0.9990 and 1.0000, the average recovery was 99.44% and 96.30%, while the RSD was 4.04% and 3.61% respectively. In 22 batches of L. barbarum samples, the contents of zeaxanthin dipalmitate and 2-O-(β-D-glucopyranosyl) ascorbic acid were up to 0.51% and 1.33%, respectively. Conclusion: The two methods were accurate and reliable, which could be used to control the quality of L.barbarum fruit.
- Lycium barbarum L.,
- zeaxanthin dipalmitate,
- 2-O-(β-D-glucopyranosyl) ascorbic acid,
- HPLC,
- UPLC

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References (29)

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