The Modulated Role of TG-DHA Liposome on the Appetite of High Fat Diet-fed Mice

YANG Ruili; LIU Fang; ZHANG Hui; ZHOU Sainan; WANG Zhiguang; LU Na; TANG Qingjuan

doi:10.13386/j.issn1002-0306.2020080274

Volume 42 Issue 12

Jun. 2021

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Science and Technology of Food Industry > 2021 > 42(12): 340-346. > DOI: 10.13386/j.issn1002-0306.2020080274

YANG Ruili, LIU Fang, ZHANG Hui, et al. The Modulated Role of TG-DHA Liposome on the Appetite of High Fat Diet-fed Mice[J]. Science and Technology of Food Industry, 2021, 42(12): 340−346. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306. 2020080274.

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The Modulated Role of TG-DHA Liposome on the Appetite of High Fat Diet-fed Mice

College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

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Received Date: August 26, 2020
Available Online: April 27, 2021

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Abstract

Abstract

Objective: To prepare TG-DHA liposome and investigate its effect on the expression of appetite regulating factors in high fat diet-fed mice. Methods: Firstly, TG-DHA liposomes were prepared by thin film dispersion method. Subsequently, C57BL/6J mice were housed and randomly divided into control group (C), model group (M), and TG-DHA liposome group (L-DHA). The gavage dose was 1000 mg/kg.bw. After 8 weeks gavage, Serum leptin, insulin and proopiomelanocortin (POMC) were detected using ELISA; The mRNA expression of neuropeptide Y (NPY) 、α-melanocyte stimulating hormone (α-MSH) 、cholecystokinin (CCK)、peptide YY (PYY) and glucagon like peptide-1 (GLP-1) were determined using RT-PCR method. Results: The encapsulation efficiency of TG-DHA liposomes was 85.83%. Compared with the M group, the L-DHA group significantly improved the content of serum leptin (23.03%) and POMC (1.30) (P<0.01), and the mRNA expression levels of α-MSH (1.60)、PYY (1.32) and GLP-1 (78.10%) (P<0.05). Conclusion: TG-DHA liposome could improve the abnormal expression of appetite regulating factors such as serum appetite hormones, hypothalamic neuropeptides and brain-gut peptides, inhibit the appetite of high-fat diet mice, thereby improving obesity induced by high-fat diet.
- TG-DHA liposome,
- DIO-HF,
- appetite,
- regulation,
- proopiomelanocortin(POMC),
- peripheral hormone,
- brain gut peptide

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