Determination of Rosin in Meat and Their Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry

WU Wanqin; JIANG Feng; FAN Xiaolong; ZHU Xiaoling; HUANG Kun; HAN Zhi; LIU Guojiao; ZHANG Yazhen; ZHU Songsong; FAN Zhiyong; WANG Huixia

doi:10.13386/j.issn1002-0306.2020080257

Volume 42 Issue 12

Jun. 2021

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Science and Technology of Food Industry > 2021 > 42(12): 279-286. > DOI: 10.13386/j.issn1002-0306.2020080257

WU Wanqin, JIANG Feng, FAN Xiaolong, et al. Determination of Rosin in Meat and Their Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(12): 279−286. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080257.

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Determination of Rosin in Meat and Their Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry

WU Wanqin^{1, 2,},
JIANG Feng^{1, 2, ,},
FAN Xiaolong^{1, 2},
ZHU Xiaoling^{1, 2},
HUANG Kun^{1, 2},
HAN Zhi^{1, 2},
LIU Guojiao^{1, 2},
ZHANG Yazhen^{1, 2},
ZHU Songsong^{1, 2},
FAN Zhiyong^{1, 2},
WANG Huixia^{1, 2}

1.
Hubei Provincial Institute for Food Supervision and Test, Wuhan 430075, China
2.
Hubei Provincial Engineering andTechnology Research Center for Food Quality and Safety Test, Wuhan 430075, China

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Received Date: August 25, 2020
Available Online: April 01, 2021

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Abstract

Abstract

To establish a method for the determination of rosin in meat and their products by using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), the sample was extracted by ultrasonic instrument with acetonitrile. The extracts were then purified through solid-phase extraction (SPE) with C₁₈ cartridges. The target of dehydroabietic acid was analyzed by HPLC-MS/MS, and quantified by internal standard method. The separation was performed on a Waters ACQUITY HSS T3 column (2.1 mm×100 mm, 1.8 μm) with isometric elution using 0.1%(v/v)formic acid aqueous solution-acetonitrile (30:70) as the mobile phase.The analysis of dehydroabietic acid was detected under electrospray positive ionization mode and the multiple reaction monitoring(MRM) mode. The dehydroabietic acid demonstrated good linearity in the range of 5~400 ng/mL, with the correlation coefficient values (r) being higher than 0.99. The limits of detection (LODs) and limits of quantification (LOQs) were found to be 80 and 200 μg/kg. The recoveries at three spiked levels (200, 400 and 2000 μg/kg) in blank matrix were in the range of 96.05%~104.17% with the relative standard deviations between 0.62%~3.68% (n=6). The analysis results of actual samples showed that the number of positive samples of dehydroabietic acid accounted for 16.67% of the total samples, and the residual content was in the range of 390.15~1850.03 μg/kg. The method was convenient, rapid, accurate and efficient. It could be employed for accurate determination of rosin in meat and their products.
- high performance liquid chromatography-tandem mass spectrometry,
- rosin,
- dehydroabietic acid,
- meat and their products

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References (31)

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