Study on Preparation of Mixed Beverage of Dendrobium candidum Polysaccharide and Hawthorn and Its Effect on Relieving Exercise Fatigue

A new formula of compound beverage was developed by polysaccharides of Dendrobium candidum and hawthorn juice as raw materials. On the basis of single factor tests, analyzing the optimum parameters of the mixed beverage byorthogonal test and response surface method, then measuring the exhaustive swimming time and related biochemical indexes in different groups of mice, respectively. Results showed that, the optimum parameters of mixed beverage were as follows: The volume ratio of polysaccharide extract of Dendrobium candidum and hawthorn juice was 1:3, the amount of sucrose was 4%, the amount of citric acid was 0.4%, the amount of sodium carboxymethyl cellulose was 0.20%, the amount of sodium alginate was 0.08%, and the amount of xanthan gum was 0.04%. The sensory evaluation score and centrifugal sedimentation rate of the beverage that optimum process preparation was (91.7 ± 0.4) scores and 0.61%± 0.02%. The beverage solution presented a light yellow-green color, transparent, and smooth taste with the proper acidity and sweet, without obvious precipitation or stratification after storage, and had strong mixed fragrance of Dendrobium candidum and hawthorn. Compared with the blank control group, the exhaustive swimming time of the groups of compound beverage at middle and high doses could separately prolong 1.5 min (P<0.05) and 4.4 min (P<0.01) in mice, the BLA concentration respectively decreased 1.2 mmol/L (P<0.05) and 3.4 mmol/L (P<0.01) in vivo, and BUN concentration separately decreased 2.6 mmol/L (P<0.01) and 3.5 mmol/L (P<0.01) in vivo. Therefore, the mixed beverage of Dendrobium candidum polysaccharide and hawthorn had better relieve exercise fatigue, and could be used in the field of sports nutrition.