Optimization of the Ultrasound Extraction Technology and Antioxidant Activity Analysis of the Total Flavonoids from <i>Debregeasia orientalis</i> Leaves by Box-Behnken Method

GAO Linxiao; MA Wensheng; SHI Huili; GUO Meng; YANG Zaibo

doi:10.13386/j.issn1002-0306.2020080230

Volume 42 Issue 12

Jun. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(12): 184-190. > DOI: 10.13386/j.issn1002-0306.2020080230

GAO Linxiao, MA Wensheng, SHI Huili, et al. Optimization of the Ultrasound Extraction Technology and Antioxidant Activity Analysis of the Total Flavonoids from Debregeasia orientalis Leaves by Box-Behnken Method[J]. Science and Technology of Food Industry, 2021, 42(12): 184−190. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080230.

Citation:

PDF (1634 KB)

Optimization of the Ultrasound Extraction Technology and Antioxidant Activity Analysis of the Total Flavonoids from Debregeasia orientalis Leaves by Box-Behnken Method

GAO Linxiao^{1, 2,},
MA Wensheng¹,
SHI Huili³,
GUO Meng^1, ,,
YANG Zaibo^{1, 2}

1.
School of Chemistry and Chemical Engineering, Qiannan Normal College for Nationalities, Duyun 558000, China
2.
Guizhou Province College Research Center of Ethnical Medicinal Plant Resources Exploitation Engineering, Duyun 558000, China
3.
China National Research Institute of Food & Fermentation Industries Corporation Limited, Beijing 100015, China

More Information

Received Date: August 23, 2020
Available Online: March 31, 2021

Graphical Abstract

Abstract

Abstract

Using Debregeasia orientalis leaves as raw materials, the Box-Behnken model was designed by single factor experiments and the optimal technological conditions for extracting total flavonoids from Debregeasia orientalis leaves were optimized, and the antioxidant activity in vitro was evaluated. The results showed that the ethanol concentration, liquid-material ratio, ultrasonic time and ultrasonic temperature were 35%, 42 mL/g, 47 min and 70 ℃, respectively, which were consistent with the predicted values of Box-Behnken model. The average extraction rate of total flavonoids from Debregeasia orientalis leaves was 3.15%±1.82%. Under these conditions, the total antioxidant capacity of extract was equivalent to 1.969 mmol/L (FeSO₄ equivalent value), and the IC₅₀ of DPPH free radical scavenging rate was 0.075 mg/mL, which indicated that the total flavonoids of the extract from Debregeasia orientalis leaves could exhibit good antioxidant activity, and provide certain theoretical data support for further research and development of wild resources of Debregeasia orientalis.
- Debregeasia orientalis leaves,
- total flavonoids,
- ultrasound extraction,
- Box-Behnken model,
- antioxidant activity

FullText(HTML)

References (30)

References

[1]	中国科学院中国植物志编辑委员会. 中国植物志[M]. 北京: 科学出版社, 1995: 394−395.
[2]	张应团, 易咏梅, 王贞. 水麻扦插的插穗类型与扦插时间效应分析[J]. 安徽农业科学,2013,41(13):5713−5715. doi: 10.3969/j.issn.0517-6611.2013.13.027
[3]	晁无疾, 陈志平. 野生果树水麻资源研究[J]. 中国野生植物,1992(4):1−4.
[4]	王坚. 水麻属植物研究现状及其开发价值[J]. 中国民族民间医药,2011,20(12):48−49. doi: 10.3969/j.issn.1007-8517.2011.12.034
[5]	王国良, 李建科, 吴晓霞, 等. 水麻果多酚的提取纯化及其抗氧化、抗肿瘤活性作用[J]. 天然产物研究与开发,2019(31):1−9.
[6]	Ullah S, Ibrar M, Barkatullah, et al. Antinociceptive and anti-diarrheal activities of Debregeasia salicifolia[J]. J Chem Soc Pakistan,2014,36:1129−1132.
[7]	肖艳华, 曹辉, 张国林. 水麻的化学成分研究[J]. 天然产物研究与开发,2008,20(1):52−55. doi: 10.3969/j.issn.1001-6880.2008.01.013
[8]	王玲玲, 边祥雨, 高蔚娜, 等. 植物类黄酮提取纯化技术研究进展[J]. 天然产物研究与开发,2019,41(6):606−610.
[9]	Laghari A Q, Memon S, Nelofar A, et al. Extraction, identification and antioxidative properties of the flavonoid-rich fractions from leaves and flowers of Cassia angustifolia[J]. Am J Anal Chem,2011,2:871−878. doi: 10.4236/ajac.2011.28100
[10]	Lamou B, Taiwe G S, Hamadou A, et al. Antioxidant and antifatigue properties of the aqueous extract of Moringa oleifera in rats subjected to forced swimming endurance test[J]. Oxid Med Cell Longev,2016,1:1−9.
[11]	郝可欣, 胡文忠, 张清洁, 等. 响应面法优化超声辅助提取代代花总黄酮的工艺及其抗氧化活性研究[J]. 食品工业科技,2019,40(24):159−164.
[12]	刘娜, 陈灵智, 张彦, 等. 响应面优化超声提取汉麻大麻素及其抗氧化性研究[J]. 现代化工,2019,39(1):144−148.
[13]	宋林晓, 邵娟娟. 黄酮类化合物提取方法研究进展[J]. 粮食与油脂,2020,33(1):21−22. doi: 10.3969/j.issn.1008-9578.2020.01.008
[14]	吴文博, 董占军. 中药制剂中细胞破壁技术探讨[J]. 中国药房,2011,22(3):285−287.
[15]	蔡凌云, 黎云祥, 陈焦, 等. 白簕根皮总黄酮提取工艺研究[J]. 食品科学,2009,30(4):15−18.
[16]	陈虎, 蒲俊松, 向仲怀, 等. 药桑葚总黄酮的提取工艺及其抗氧化活性分析[J]. 食品科学,2014,35(12):7−12. doi: 10.7506/spkx1002-6630-201412002
[17]	孔繁晟, 贲永光, 孙爱群, 等. 超声法与连续回流法提取黄芪总黄酮的工艺对比研究[J]. 中国药房,2010,21(19):1752−1754.
[18]	谢蓝华, 杜冰, 叶琼娟, 等. 正交试验优化酶法提取黄芪总黄酮工艺[J]. 包装与食品机械,2013,31(3):7−12. doi: 10.3969/j.issn.1005-1295.2013.03.002
[19]	李钢, 彭飞, 尹洪洋, 等. 板栗叶总黄酮提取工艺的优化及其抗氧化活性[J]. 食品研究与开发,2020,41(3):64−72.
[20]	Choudhary A, Kumar R, Srivastava R B, et al. Isolation and characterization of phenolic compounds from Rhodiola imbricata, a trans-himalayan food crop having antioxidant and anticancer potential[J]. J Funct Foods,2015,16:183−193.
[21]	陈建福. 响应面优化超声辅助提取黄槿叶总黄酮工艺及其亚硝酸盐清除能力[J]. 食品工业科技,2019,40(6):193−197.
[22]	I lhami G, Mehmet Emin B. Metal chelating and hydrogen peroxide scavenging effects of melatonin[J]. J Pineal Res,2010,34(4):278−281.
[23]	张玲玲, 孙芬芳, 张蓉希, 等. 响应面法优化提取丁香中的总黄酮及抗氧化性研究[J]. 中国调味品,2019,44(11):40−50. doi: 10.3969/j.issn.1000-9973.2019.11.009
[24]	王芳, 乔璐, 淡小艳, 等. 桑树黄酮的提取及抗氧化研究[J]. 广东农业科学,2011,38(15):76−79. doi: 10.3969/j.issn.1004-874X.2011.15.029
[25]	徐洪宇, 蒯宜蕴, 赵烊, 等. 响应面优化提取龙牙楤木中总黄酮的工艺研究[J]. 食品研究与开发,2019,40(3):106−111. doi: 10.3969/j.issn.1005-6521.2019.03.018
[26]	汤磊, 刘本. 响应面法优化白芍中芍药普的超临界流体提取研究[J]. 中成药,2010,32(8):1332−1336. doi: 10.3969/j.issn.1001-1528.2010.08.016
[27]	张迪. 响应面法优化马鞭草中总黄酮闪式提取工艺及其体外抗氧化活性[J]. 食品工业科技,2019,40(3):173−178.
[28]	张斌, 陈兆贵, 邹俊杰. 响应面法优化荔枝醋酸发酵的工艺条件[J]. 中国酿造,2019,38(6):136−139. doi: 10.11882/j.issn.0254-5071.2019.06.026
[29]	曹艳华, 程丽丽, 张小芳. 响应面法优化洋葱多酚的提取工艺及抗氧化性研究[J]. 食品研究与开发,2020,41(12):164−170. doi: 10.12161/j.issn.1005-6521.2020.12.027
[30]	张锦东, 王小玉, 游淑珠, 等. 菠萝蜜种子中总黄酮的提取工艺及其抗氧化性研究[J]. 广东农业科学,2017,44(12):136−143.

Supplements (0)

Cited By

Cited by

Periodical cited type(2)

1.	谭婉静，张笑薇，白泓，李晓凤，余以刚，肖性龙. 丁香油与月桂酸对金黄色葡萄球菌的协同抑制作用. 现代食品科技. 2022(02): 278-285+265 .
2.	于航，王海默，钟世勋，吕月琴，刘兆胜，孙汝江. 抗菌肽APSH-07与抗生素对常见致病菌的体外协同抗菌效果研究. 饲料工业. 2021(16): 22-26 .