Evaluation of in Vitro Anti-inflammatory and Antioxidant Activities and Analysis of Chemical Components in Different Extraction Parts of Lonicerae Flos

The aim of this study is to elucidate the material basis of Lonicerae Flos biological activity and provide a theoretical basis for its development and utilization, lipopolysaccharide (LPS)-induced cell inflammation model and H₂O₂-induced oxidative stress model were used to evaluate the anti-inflammatory and antioxidant activities of different extraction parts of Lonicerae Flos. The chemical constituents of the extractions with significant activities were analyzed by ultra-high pressure liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The results showed that the methanol extracts of Lonicerae Flos(50 μg/mL) have different levels of anti-oxidation and anti-inflammatory effects. The extraction site of saturated n-butanol effectively inhibited the inflammatory factors (NO、TNF-α and IL-6) of RAW264.7 cells, suggesting it has the strongest anti-inflammatory activity. The inhibition rates of inflammatory factors of NO, TNF-α and IL-6 were 66.40%, 13.14% and 79.66% respectively. 16 compounds in the water-saturated n-butanol extractive site were identified, and which mainly consisted of organic acids.The ethyl acetate extractive site has the strongest antioxidant ability. The antioxidant enzyme activity in RIN cells had increased about 2.51(SOD), 4.40(GSH), 8.89(CAT) times respectively. After preliminary identification, 12 compounds, including organic acids and saponins in the ethyl acetate site, were the main compound. Therefore, organic acids and saponins were the main chemical components of Lonicerae Flos, which had the antioxidant and anti-inflammatory activities.This article provides data support for further elucidating the material basis and development and utilization of Lonicerae Flos.