Anti Exercise Fatigue and Antioxidant of Polysaccharide from Hippophae rhamnoides

To study the effect of polysaccharide from Hippophae rhamnoides (PBP) on mitigating physical fatigue in mice with prolonged excessive exercise. The mice were divided into blank group, low (100 mg/kg), middle (200 mg/kg) and high (400 mg/kg) dose group of PBP, and fed continuous gavage for 15 d. Exhaustive swimming time was recorded. Then, the levels of blood lactic acid (LA), blood urea nitrogen (BUN) and the contents of glycogen. The results showed that, compared with the blank group, PBP significantly prolonged exhaustive swimming time (P<0.05). Exhaustive swimming time in middle PBP group increased significantly (P<0.01). And it significantly decreased the levels of BUN and LA in middle and high PBP groups. The level of HG in middle PBP group increased significantly (P<0.01). The antioxidant activity experiment in vitro showed that, the polysaccharideof Hippophae rhamnoides had certain scavenging ability to DPPH and ABTS⁺ free radical. And the level of IC₅₀ was 0.81 and 2 mg/mL. When the concentration of polysaccharide of Hippophae rhamnoides was 1 mg/mL, the total reducing power was 0.727. Therefore, the polysaccharide of Hippophae rhamnoides can relieve physical fatigue, enhance the exercise ability of mice and have effect on antioxidant.