DENG Xiujuan, TU Qing, WU Xianxue, et al. Research Progress on the Safety Risk of Ochratoxin A in Tea [J]. Science and Technology of Food Industry, 2021, 42(12): 405−412. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020070080.
Citation: DENG Xiujuan, TU Qing, WU Xianxue, et al. Research Progress on the Safety Risk of Ochratoxin A in Tea [J]. Science and Technology of Food Industry, 2021, 42(12): 405−412. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020070080.

Research Progress on the Safety Risk of Ochratoxin A in Tea

More Information
  • Received Date: July 08, 2020
  • Available Online: April 12, 2021
  • Tea is one of the most consumed drinks in the world. Its drinking safety is particularly important for consumer's health and the development of tea industry. In recent years, the contamination of tea with mycotoxin has aroused widespread concern in the society, how to deal with this problem scientifically and objectively is crucial. Ochratoxin A(OTA) is a highly harmful fungal toxin. It’s produced by a wide variety of fungus, soluble in water and not easy to degrade, and has strong hepatorenal toxicity, teratogenic, mutagenic, carcinogenic and immunosuppressive effects. In this paper, the physicochemical characteristics, harm and toxicological mechanism, main toxogenic fungus and toxogenic conditions of OTA, possible OTA-producing microorganisms in tea as well as OTA detection methods and results are summarized. The potential risks of OTA in tea are analyzed and discussed, and suggestions are put forward to provide reference for the risk assessment, prevention and control of OTA contamination in tea.
  • [1]
    Marin S, Ramos A J, Cano-Sancho G, et al. Mycotoxins: Occurrence, toxicology, and exposure assessment[J]. Food Chem Toxicol,2013,60:218−237. doi: 10.1016/j.fct.2013.07.047
    [2]
    Bhat R, Rai R V, Karim A A. Mycotoxins in food and feed: Present status and future concerns[J]. Compr Rev Food Sci Food Saf,2010,9(1):57−81. doi: 10.1111/j.1541-4337.2009.00094.x
    [3]
    Van der Merwe K J, Steyn P S, Fourie L. Ochratoxin A, a toxic metabolite produced by Aspergillus ochraceus Wilh[J]. Nature,1965,205(976):1112−1113.
    [4]
    Annie P L, Richard A, Manderville. Ochratoxin A: An overview on toxicity and carcinogenicity in animals and humans[J]. Mol Nutr Food Res,2007,51:61−99. doi: 10.1002/mnfr.200600137
    [5]
    IARC. Some naturally occurring substances: Food items and constituents, heterocyclic aromatic amines and mycotoxins[M]. World Health Organization, 1997.
    [6]
    姜依何, 胥伟, 朱旗. 黑茶真菌污染研究进展及探讨[J]. 茶叶科学,2018,38(3):227−236. doi: 10.3969/j.issn.1000-369X.2018.03.002
    [7]
    Jonathan P H, Peter G M. Biosynthesis of ochratoxins by Aspergillus ochraceus[J]. Phytochemistry,2001,58:709−716. doi: 10.1016/S0031-9422(01)00316-8
    [8]
    Kong W J, Wei R W, Antonio F L, et al. Occurrence of toxigenic fungi and determination of mycotoxins by HPLC-FLD in functional foods and spices in China markets[J]. Food Chemistry,2014,146:320−326. doi: 10.1016/j.foodchem.2013.09.005
    [9]
    Antonia G, Kenneth S B, Michele S, et al. New insight in the ochratoxin A biosynthetic pathway by deletion of an nrps gene in Aspergillus carbonarius[J]. Applied & Environmental Microbiology,2012,78:1−37.
    [10]
    郝俊冉, 许文涛, 黄昆仑. 赭曲霉毒素A生成转化及致毒机制的研究进展[J]. 食品工业科技,2012,33(12):427−433.
    [11]
    彭娅萍, 杨其亚, 张晓云, 等. 葡萄上赭曲霉毒素A产生机制及生物检测方法研究进展[J]. 食品与发酵工业,2016,42(6):236−242.
    [12]
    王健, 成桂红, 阮若云, 等. 动物源性食品中赭曲霉毒素A污染概况及检测方法研究进展[J]. 食品安全质量检测学报,2018,9(16):4212−4217. doi: 10.3969/j.issn.2095-0381.2018.16.005
    [13]
    Alborch L, Bragulat M, Abarca M, et al. Effect of water activity, temperature and invubation time on growth and ocharatxin A production by Aspergillus niger and Aspergillus cabonarius on maize kemels[J]. International Journal of Food Microbiology, 2011, 147: 53−57.
    [14]
    Ostry V, Malir F, Dofkova M, et al. Ochratoxin A dietary exposure of ten population groups in the czech republic: Comparison with data over the world[J]. Toxins,2015,7(9):3608−3635. doi: 10.3390/toxins7093608
    [15]
    Mitchell N J, Chen C, Palumbo J D, et al. A risk assessment of dietary ochratoxin A in the United States[J]. Food Chem Toxicol,2017(100):265−273.
    [16]
    Cappozzo J, Jackson L, Lee H J, et al. Occurrence of ochratoxin A in infant foods in the United States[J]. J Food Protect,2017,80(2):251. doi: 10.4315/0362-028X.JFP-16-339
    [17]
    Kamali A, Mehni S, Kamali M, et al. Detection of ochratoxin A in human breast milk in Jiroft city, south of Iran[J]. Curr Med Mycol,2017,3(3):1−4. doi: 10.29252/cmm.3.3.1
    [18]
    Pattono D, Gallo P F. Civera T. Detection and quantification of ochratoxin A in milk produced in organic farms[J]. Food Chemistry,2011,127(1):374−377. doi: 10.1016/j.foodchem.2010.12.051
    [19]
    Malir F, Ostry V, Pfohl-Leszkowicz A. Ochratoxin A: 50 years of research[J]. Toxins,2016,8(7):191. doi: 10.3390/toxins8070191
    [20]
    Annie P L, Petkova-Bocharova T, Chernozemsky IN, et al. Balkan endemic nephropathy and associated urinary tract tumours: A review on aetiological causes and the potential role of mycotoxins[J]. Food Addit Contam,2002,19(3):282−302. doi: 10.1080/02652030110079815
    [21]
    Annie P L, Manderville R A. Ochratoxin A: An overview on toxicity ande carcinogenicity in animals and humans[J]. Molecular Nutrition& Food research,2007,51:61−99.
    [22]
    佟翠, 张诗萌, 李鹏, 等. 赭曲霉毒素A免疫毒性研究进展[J]. 动物医学进展,2019,40(3):106−109.
    [23]
    陈光明, 刘建军, 刘桂兰, 等. 霉菌毒素的研究进展[J]. 畜牧与饲料科学,2014,35(12):122−124. doi: 10.3969/j.issn.1672-5190.2014.12.063
    [24]
    刘彬. 不同温度和湿度下赭曲霉菌在葡萄干表面的生长和赭曲霉毒素A的积累[J]. 食品研究与开发,2010,31(10):174−177. doi: 10.3969/j.issn.1005-6521.2010.10.052
    [25]
    European Commission. Commission Regulation (EC). No1881/2006 amending Regulation(EC) as regards ochratoxin A[S]. Official Journal of the European Union, 2006.
    [26]
    中华人民共和国卫生部. GB 2761-2017 食品安全国家标准 食品中真菌毒素限量[S]. 北京: 中国标准出版社, 2017.
    [27]
    Duarte S C, Lino C M, Pena A. Food safety implications of ochratoxin A in animal-derived food products[J]. Vete J,2012,192(3):286−292. doi: 10.1016/j.tvjl.2011.11.002
    [28]
    Varga J, Kozakiewicz. Ochratoxin A in grapes and grape-derived products[J]. Trends in Food Science and Technology,2006,17:72−81. doi: 10.1016/j.jpgs.2005.10.007
    [29]
    王希春. 农产品中两种真菌毒素蛋白质芯片检测技术的研究[D]. 南京: 南京农业大学, 2010.
    [30]
    Duarte S, Pena A, Lino C. A review on ochratoxin A occurrence and effects of processing of cereal ande cereal derived food products[J]. Food Microbiology,2010,27:187−198. doi: 10.1016/j.fm.2009.11.016
    [31]
    Iqbal S Z, Nisar S, Asi M R, et al. Natural incidence of aflatoxins, ochratoxin A and zearalenone in chicken meat and eggs[J]. Food Control,2014,43(8):98−103.
    [32]
    Ferrara M, Magistà D, Epifani F, et al. Study of gene expression and OTA production by Penicillium nordicum, during a small-scale seasoning process of salami[J]. Int J Food Microbiol,2016(227):51−55.
    [33]
    Ferrara M, Magistà D, Lippolis V, et al. Effect of Penicillium nordicum, contamination rates on ochratoxin A accumulation in dry-cured salami[J]. Food Control,2016(67):235−239.
    [34]
    Sanchez-Hervas M, Gil J, Bisbal F, et al. Mycobiota and mycotoxin producing fungi cocoa beans[J]. International Journal of Food Microbiology,2008,125:336−340. doi: 10.1016/j.ijfoodmicro.2008.04.021
    [35]
    王海英, 张红印, 杨其亚, 等. 葡萄赭曲霉毒素污染及其产毒素菌株的筛选方法研究进展[J]. 食品工业科技,2015,36(10):369−372.
    [36]
    Swamy H V, Smith T K, Cotter P F, et al. Effects of feeding blends of grains naturally contaminated with Fusarium mycotoxins on production and metabolism in broilers[J]. Poult Sci,2002,81(7):966−975. doi: 10.1093/ps/81.7.966
    [37]
    Horie Y. Productivity of ochratoxin A of Aspergillus carbonarius in Aspergillus cection Nigri[J]. Nippon Kingakukai Kaiho,1995,36:73−76.
    [38]
    郝俊冉. 谷胱甘肽和锌离子缓解赭曲霉毒素毒性的组学研究[D]. 北京: 中国农业大学, 2015.
    [39]
    Arroyo M, Aldred D, Magan N. Environmental factors and weak organic acid interactions have differential effects on contro of growth and ochrataxin A production by Penicillium verrucosum isolates in bread[J]. International Journal of Food Microbiology,2005,98:223−231. doi: 10.1016/j.ijfoodmicro.2004.07.004
    [40]
    Esteban A, A barca M, Bragulat M, et al. Study of the effect of water activity and temperature on ocharatxin A production by Aspergillus cabonarius[J]. Food Microbiology,2006,23:634−640. doi: 10.1016/j.fm.2005.12.006
    [41]
    Dutta S, Dutta B K, Nath P K. Some observations on the aeromycoflora of tea factory in Cachar, District, Assam[J]. Assam Univ J Sci Tech,2010,4(1):13−19.
    [42]
    Dutta B K, Dutta S, Nath P K. Mycotoxin production potential of mycoflora in tea[J]. In Economic Crisis in Tea Industry,2008:221−232.
    [43]
    R ezacova V, Kubatova A. Saprobic microfungi in tea based on Camellia sinensis and on other dried herbs[J]. Czech Mycol,2005,57:79−89. doi: 10.33585/cmy.57104
    [44]
    Carraturo F, De C O, Troisi J, et al. Comparative assessment of the quality of commercial black and green tea using microbiology analyses[J]. BMC Microbiol,2018,18:4. doi: 10.1186/s12866-017-1142-z
    [45]
    Elshafie A E, Al-Lawatia T, Al-Bahry S. Fungi associated with black tea and tea quality in the Sultanate of Oman[J]. Mycopathologia,1999,145:89−93. doi: 10.1023/A:1007034729467
    [46]
    Storari M, Dennert F G, Bigler L, et al. Isolation of mycotoxins producing black Aspergilli in herbal teas available on the Swiss market[J]. Food Control,2012,26:157−161. doi: 10.1016/j.foodcont.2012.01.026
    [47]
    Zhao Z J, Tong H R, Zhou L, et al. Fungal colonisation of Pu-erh tea in Yunnan[J]. Food Safety,2010,30(4):769−784. doi: 10.1111/j.1745-4565.2010.00240.x
    [48]
    Haas D, Pfeifer B, Reiterich C, et al. Identification and quantification of fungi and mycotoxins from Pu-erh tea[J]. Int J Food Microbiol,2013,166:316−322. doi: 10.1016/j.ijfoodmicro.2013.07.024
    [49]
    Zhao Z J, Hu X C, Liu Q J. Recent advances on the fungi of Pu-erh ripe tea[J]. Intern Food Res J,2015,22:1240−1246.
    [50]
    Abe M, Takaoka N, Idemoto Y, et al. Characteristic fungi observed in the fermentation process for puer tea[J]. Int J Food Microbiol,2008,124(2):199−203. doi: 10.1016/j.ijfoodmicro.2008.03.008
    [51]
    杨瑞娟, 吕杰, 严亮, 等. 普洱茶渥堆发酵中嗜热真菌的分离和鉴定[J]. 茶叶科学,2011,31(4):371−378. doi: 10.3969/j.issn.1000-369X.2011.04.014
    [52]
    Zhao M, Zhang D, Su X, et al. An integrated metagenomics/metaproteomics investigation of the microbial communities and enzymes in solid-state fermentation of Pu-erh tea[J]. Scientific Reports,2015(5):1−10.
    [53]
    邓秀娟. 应用红曲菌发酵普洱茶的微生物群落结构及理化品质动态变化[D]. 昆明: 云南农业大学, 2016.
    [54]
    骆爱国. 普洱茶固态发酵高温阶段微生物的动态变化规律研究[D]. 昆明: 云南农业大学, 2017.
    [55]
    Zhao M, Xiao W, Ma Y, et al. Structure and dynamics of the bacterial communities in fermentation of the traditional Chinese post-fermented pu-erh tea revealed by 16S rRNA gene clone library[J]. World Journal of Microbiology and Biotechnology,2013,29(10):1877−1884. doi: 10.1007/s11274-013-1351-z
    [56]
    Lyu C, Chen C, Ge F, et al. A preliminary metagenomic study of puer tea during pile fermentation[J]. Journal of the Science of Food and Agriculture,2013,93(13):3165−3174. doi: 10.1002/jsfa.6149
    [57]
    胥伟. 四川康砖茶渥堆过程中真菌种群的鉴定[D]. 雅安: 四川农业大学, 2010.
    [58]
    赵仁亮. 茯砖茶加工中微生物演变及对品质形成影响的研究[D]. 长沙: 湖南农业大学, 2012.
    [59]
    Xu A, Wang Y, Wen J, et al. Fungal community associated with fermentation and storage of Fuzhuan brick-tea[J]. Int J Food Microbio,2011,146(1):14−22. doi: 10.1016/j.ijfoodmicro.2011.01.024
    [60]
    陈庆金, 黄丽, 滕建文, 等. 基于Miseq测序分析六堡茶陈化初期真菌多样性[J]. 食品科技,2015,40(8):67−71.
    [61]
    钟涛, 齐桂年, 胥伟, 等. 藏茶贮存过程中真菌种群的鉴定[J]. 贵州农业科学,2010,38(10):101−103. doi: 10.3969/j.issn.1001-3601.2010.10.031
    [62]
    周才碧, 陈文品, 吴钟玲, 等. 普洱茶优势菌株黑曲霉的分离及其功能和安全性研究[J]. 食品安全质量检测学报,2015,6(3):1006−1010.
    [63]
    周才碧, 陈文品, 赵振军, 等. 普洱茶优势菌株赭曲霉的功能和安全性研究[J]. 食品研究与开发,2015,36(24):160−163. doi: 10.3969/j.issn.1005-6521.2015.24.042
    [64]
    徐书泽. 六堡茶真菌的多样性分析[D]. 南宁: 广西大学, 2014.
    [65]
    Irina S, Mariya K, Victor T, ea al. Mycotoxins in Tea: Occurrence, methods of determination and risk evaluation[J]. Toxins,2018,10:444. doi: 10.3390/toxins10110444
    [66]
    Mogensen J M, Varga J, Thrane U, Frisvad J C. Aspergillus acidus from Pu-erh tea and black tea does not produce ochratoxin A and fumonisin B-2[J]. Int. J. Food Microbiol,2009,132:141−144. doi: 10.1016/j.ijfoodmicro.2009.04.011
    [67]
    Ahmet E, Selahatti S. Mycotoxin-forming ability of two penicillium roqueforti strains in blue moldy tulum cheese ripened at various temperatures[J]. Journal of Food Protection,2004,67(3):533−535. doi: 10.4315/0362-028X-67.3.533
    [68]
    Santos L, Marin S, Sanchis V, et al. Screening of mycotoxin multicontamination in medicinal and aromatic herbs sampled in Spain[J]. J Sci Food Agric,2009,89(10):1802−1807. doi: 10.1002/jsfa.3647
    [69]
    柳其芳. ELISA 法测定发酵茶和植物香料真菌毒素的污染[J]. 中国热带医学,2011,11(11):1381−1409.
    [70]
    刘妍, 谭贵良, 刘子雄, 等. 发酵茶中多种真菌毒素超高效液相色谱-串联质谱法的测定[J]. 现代食品科技,2016,32(8):322−327.
    [71]
    陈秋娥. 普洱茶中霉菌毒素之研究论文[D]. 台北: 国立台湾大学, 2003.
    [72]
    Monbaliu S, Wu A, Zhang D, et al. Multimycotoxin UPLCMS/MS for tea, herbal infusions and the derived drinkable products[J]. J Agric Food Chem,2010,58(24):12664−12671. doi: 10.1021/jf1033043
    [73]
    莫瑾, 龚强, 周慧平, 等. 高效液相色谱-串联质谱法检测茶叶中的赭曲霉毒素A[J]. 食品安全质量检测学报,2016,7(1):182−187.
    [74]
    Mogensen J M, Varga J, Thrane U, et al. Aspergillus acidus from puerh tea and black tea does not produce ochratoxin A and fumonisin B2[J]. Int J Food Microbiol,2009,132(2/3):141−144.
    [75]
    Hou C W, Jeng K C, Chen Y S. Enhancement of fermentation process in pu-erh tea by tea-leaf extract[J]. J Food Sci,2010,75(1):44−48. doi: 10.1111/j.1750-3841.2009.01441.x
    [76]
    Wang Q P, Bojan S, Jasna J, et al. Evaluation of microbial toxins, trace elements and sensory properties of a high-theabrownins instant Pu-erh tea produced using Aspergillus tubingensis via submerged fermentation[J]. International Journal of Food Science & Technology,2019,54(5):1541−1549.
    [77]
    Frantisek M, Vladimir O, Annie P L, et al. Transfer of ochratoxin A into tea and coffee beverages[J]. Toxins,2014,6:3438−3453. doi: 10.3390/toxins6123438
    [78]
    Wang Y, Zhang N N, Luo L N, et al. Research progress on detection methods of ochratoxin A[J]. Chin J Hyg Lab,2014(5):757−760.
    [79]
    Cladière M, Delaporte G, Le R E, et al. Multi-class analysis for simultaneous determination of pesticides, mycotoxins, process-induced toxicants and packaging contaminants in tea[J]. Food Chemistry,2018,242:113−121. doi: 10.1016/j.foodchem.2017.08.108
    [80]
    Al-Hazmi N. Determination of patulin and ochratoxin A using HPLC in apple juice samples in Aaudi Arabia[J]. Aaudi Journal of Biological Sciences,2010,17:353−359.
    [81]
    吴凤琪, 岳振峰, 张毅, 等. 食品中主要霉菌毒素分析方法的研究进展[J]. 色谱,2020,38(7):759−767.
    [82]
    桑晓霞, 马江媛, 黄登宇. 赭曲霉毒素A检测方法的研究进展[J]. 食品安全质量检测学报,2019,10(21):7271−7277.
    [83]
    徐超莲, 赖卫华, 刘道峰. 胶体金免疫层析法检测食品中天然存在的危害物质的研究进展[J]. 食品科学,2014,35(5):257−261.
    [84]
    龙淼, 李颜鹏, 任艳苗, 等. 微生物对赭曲霉毒素A脱毒作用研究进展[J]. 动物医学进展,2014,35(6):132−135. doi: 10.3969/j.issn.1007-5038.2014.06.028
    [85]
    贾欣, 徐诗涵, 梁志宏, 等. 赭曲霉毒素A的微生物脱毒研究进展[J]. 生物技术通报,2014,12:18−22.
    [86]
    Najim T, Katharina B, Markus S H, et al. Arginine acts as an inhibitor of the biosynthesis of several mycotoxins[J]. International Journal of Food Microbiology,2016,235:46−52. doi: 10.1016/j.ijfoodmicro.2016.06.036
  • Cited by

    Periodical cited type(2)

    1. 谭婉静,张笑薇,白泓,李晓凤,余以刚,肖性龙. 丁香油与月桂酸对金黄色葡萄球菌的协同抑制作用. 现代食品科技. 2022(02): 278-285+265 .
    2. 于航,王海默,钟世勋,吕月琴,刘兆胜,孙汝江. 抗菌肽APSH-07与抗生素对常见致病菌的体外协同抗菌效果研究. 饲料工业. 2021(16): 22-26 .

    Other cited types(3)

Catalog

    Article Metrics

    Article views PDF downloads Cited by(5)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return