Evaluation and Analysis of the Colloidal Gold Immunochromatography Kits for the Rapid Detection of Pesticide Residue

In this work,a quality evaluation method for rapid detection kits was established. The blind samples were prepared by spiking blank matrices and the homogeneity and the stability of them were verified simultaneously. Three different batches of rapid detection kits were randomly selected from each product,and each one was used to detect 50 blind samples which contained at least 20 positive and 20 negative samples. The specificity and accuracy(false positive and false negative)and other parameters of 14 commercially available assay kits for the rapid screening of pesticide residue were evaluated with a total of 2100 blind samples. The results showed that,11 of 14 of rapid detection kits passed the evaluation with a pass rate of 78.6%. One product had a higher false positive rate and two had a higher false negative rate;all the products had good specificity.This method could scientifically and objectively evaluate the quality of rapid testing products,and then screen out the rapid testing products with stable and reliable quality,and would provide technical support for grassroots regulatory authorities in the supervision of fruit and vegetable products.