Indirect Competitive ELISA for Detection of Amantadine,Rimantadine and Soramantadine in Chicken and Duck Meat

Objective: To establish an ic-ELISA for the determination of amantadine,rimantadine and soramantadine in chicken and duck meat. Methods: Based on the principle of ic-ELISA,conjugated antigen was pre-coated in the wells of microtiter plate. Amantadine,rimantadine,and soramantadine contained in the samples will competitively combine with the enzyme-labelled antibody,which catalyzing colour reactions of the substrates. The concentration of amantadines in the sample was detremined on the basis of OD value. Results: The detection limits of amantadine,rimantadine and somantine were respectively 0.57,0.42,0.41 μg/kg(chicken),and 0.59,0.40,0.38 μg/kg(duck). The limits of quantification were respectively 0.85,0.63,0.69 μg/kg(chicken),and 0.94,0.68,0.52 μg/kg(duck). The added recovery range was 67.0%~117.9%;the intra-day and inter-day variation were 6.3%~12.7% and 8.1%~14.5%,respectively. The actual sample detection results were well consistent with HPLC-MS(R²=0.9990),showing that the method had good accuracy and precision. Conclusion: The ic-ELISA method established in this study is suitable for the detection of amantadine residues in chicken and duck meat samples. The method has high sensitivity and good stability. It can be used for rapid screening of batch samples and has good practical applications value.