YANG Zheng-feng, CHEN Xin-yu, CUI Yi-dan, YANG Jin-qing, TANG Dan-yu, ZHAO Zheng-xiong, BAO Zhi-juan. Optimization of Extraction Technology and Spectrophotometric Determination Method of Crude Fat in Flue-cured Tobacco[J]. Science and Technology of Food Industry, 2020, 41(24): 219-225. DOI: 10.13386/j.issn1002-0306.2020030083
Citation: YANG Zheng-feng, CHEN Xin-yu, CUI Yi-dan, YANG Jin-qing, TANG Dan-yu, ZHAO Zheng-xiong, BAO Zhi-juan. Optimization of Extraction Technology and Spectrophotometric Determination Method of Crude Fat in Flue-cured Tobacco[J]. Science and Technology of Food Industry, 2020, 41(24): 219-225. DOI: 10.13386/j.issn1002-0306.2020030083

Optimization of Extraction Technology and Spectrophotometric Determination Method of Crude Fat in Flue-cured Tobacco

  • In order to optimize the determination parameters of crude oil from flue-cured tobacco leaves extracted by Soxhlet extraction,the single factor tests and a Box-Behnken design were applied to study the effects of sample weight,reflow time,and drying time on the extraction rate of crude oil. And a new UV absorption spectroscopic method of determination crude oil was established. The results showed that when the drying temperature was set at 105 ℃,the predictable optimum extraction conditions for crude oil were as follows:Sample weight 3.08 g,reflow time 6 h,and drying time 1.5 h. Under the optimum condition,the total extraction yield was 6.27%. The linear relationships between values of absorption and mass concentration of crude oil at 326 and 415 nm wavelength were good(R326 nm2=0.9982,R415 nm2=0.9993),with linearity ranges of 0.276~2.211 g/L(326 nm wavelength)and 0.276~2.763 g/L(415 nm wavelength). The detection limits were 0.128 and 0.093 g/L,and quantification were 0.387 and 0.281 g/L,respectively. The optimum Soxhlet extraction process and contents determination of crude fat in tobacco were established by response surface methodology and UV spectrophotometry. The method was simple,time-saving,and had good repeatability and precision,which would be suitable for analysis crude fat of batch tobacco sample.
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