Protective Effects of Sporoderm-broken Spore Powder of <i>Haematococcus pluvialis</i> on Liver and Kidney of Type 2 Diabetes Rats

BI Cui-cui; LIU Yin-lu; YANG Li-tao; WEI Fen-fen; LI Yong-chao; ZHANG Bo

doi:10.13386/j.issn1002-0306.2020020325

Volume 42 Issue 1

Dec. 2020

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Science and Technology of Food Industry > 2021 > 42(1): 328-333. > DOI: 10.13386/j.issn1002-0306.2020020325

BI Cui-cui, LIU Yin-lu, YANG Li-tao, WEI Fen-fen, LI Yong-chao, ZHANG Bo. Protective Effects of Sporoderm-broken Spore Powder of Haematococcus pluvialis on Liver and Kidney of Type 2 Diabetes Rats[J]. Science and Technology of Food Industry, 2021, 42(1): 328-333. DOI: 10.13386/j.issn1002-0306.2020020325

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Protective Effects of Sporoderm-broken Spore Powder of Haematococcus pluvialis on Liver and Kidney of Type 2 Diabetes Rats

Beijing Key Laboratory of Bioactive Substance and Functional Food, Beijing Union University, Beijing 100191, China

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Received Date: March 01, 2020
Available Online: January 07, 2021

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Abstract

Objective: To study the protective effect of sporoderm-broken spore powder of Haematococcus pluvialis on liver and kidney in type 2 diabetes rats. Methods: SD rats were divided into normal group and model group. The normal group was fed with ordinary feed,and 70 rats were modeled with high-fat and high-sugar diet for 4 weeks. Then intraperitoneal injection of streptozotocin 35 mg/kg BW;continued feeding for 4 weeks,and the blood glucose was measured to divide the successful model rats into model group,low dose group,medium dose group,high dose group,and hypoglycemic drug group. The rats in model group were administrated with double distilled water,meanwhile,the low,medium,and high dose groups were administered orally to 50,100 and 200 mg/kg BW,respectively,the rats in hypoglycemic drug group were administered orally with metformin hydrochloride(28.5 mg/kg BW),and continued to be fed with high-fat and high-sugar feed for 4 weeks. The rats were sacrificed. The alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),lactate dehydrogenase(LDH)activities,albumin(ALB)and globulin(GLO)contents of the serum were determined. Liver and kidney were obtained for determining the related markers,including superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),malondialdehyde(MDA),and the inflammatory factor levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β).Liver and kidney HE stained pathological sections were made to observe the liver and kidney histopathological changes. Results: Compared with the model group,rats in treatment groups had a significance reduction in serum ALT,AST,ALP,LDH activities,GLO content(P<0.01)and ALB content(P<0.05)in type 2 diabetes rats. The treatment groups significantly increased the activity of SOD and GSH-Px in liver and kidney tissues(P<0.01),reduce the content of MDA(P<0.01),and significantly reduce the levels of TNF-α and IL-1β(P<0.01). Furthermore,the pathological injury of liver and kidney tissues were also significantly improved. Conclusion: Sporoderm-broken spore powder of Haematococcus pluvialis can ameliorate the liver and kidney damage in type 2 diabetes rats. The mechanism may be related to reducing oxidative stress and reducing inflammatory responses.
- sporoderm-broken spore powder of Haematococcus pluvialis,
- type 2 diabetes,
- liver,
- kidney,
- rat,
- protective effects

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[1]	Richard P,Shin P,Beeson T,et al. Quality and cost of diabetes mellitus care in community health centers in the United States[J]. PLoS One,2015,10(12):e0144075.
[2]	Lebovitz Harold E. Type 2 diabetes:An overview[J]. Clinical Chemistry,1999,45(8):1339-1345.
[3]	Paul F,David F,Peter W,et al. Overweight and obesity in type 1 diabetes equal those of the general population[J]. Wiener klinische Wochenschrift,2019,131:55-60.
[4]	Zhai Jing-hui,Wu Yi,Wang Xiao-ying,et al. Antioxidation of cerium oxide nanoparticles to several series of oxidative damage related to type Ⅱ diabetes mellitus in vitro[J]. Medical Science Monitor International Medical Journal of Experimental & Clinical Research,2016,22:3792-3797.
[5]	Tan M H,Don J,Glazer N B. Pioglitazone reduces atherogenic index of plasma in patients with type 2 diabetes[J]. Clinical Chemistry,2004,50(7):1184-1188.
[6]	Shi C H,Wang C,Bai R,et al. Associations among glycemic excursions,glycated hemoglobin and high-sensitivity c-reactive protein in patients with poorly controlled type 2 diabetes mellitus[J]. Experimental and Therapeutic Medicine,2015,10(5):1937-1942.
[7]	Akiko T,Isao U,Shiho F,et al. Macrophage:Pecific HIF deletion suppresses the development of liver tumors in high fat diet-fed obese and diabetic mice[J]. Journal of Diabetes Investigation,2019,18:160-167.
[8]	刘芬. 抗氧化剂对2型糖尿病的预防作用及其分子机制[D]. 杭州:浙江工业大学,2009.
[9]	Lorenz R T,Cysewski G R. Commercial potential for Haematococcus microalgae as a natural source of astaxanthin[J]. Tibtech,2000,18:160-167.
[10]	Andreas B,Mirjana M. Direct extraction of astaxanthin from the microalgae Haematococcus pluvialis using liquid-liquid chromatography[J]. RSC Advances,2019,9(40):22779-22789.
[11]	Ursoniu S,Sahebkar A,Serban M C,et al. Lipid profile and glucose changes after supplementation with astaxanthin:A systematic review and meta-analysis of randomized controlled trials[J]. Archives of Medical Science,2015,11(2):253-266.
[12]	Ciccone M M,Cortese F,Gesualdo M,et al. Dietary intake of carotenoids and their antioxidant and anti-inflammatory effects in cardiovascular care[J]. Mediators of Inflammation,2013,2013(6):782137.
[13]	方婷. 雨生红球藻中虾青素类成分的提取分离及活性评价[D].广州:广东药科大学,2019.
[14]	Murai Tae,Kawasumi Koh,Tominaga Kumi,et al. Effects of astaxanthin supplementation in healthy and obese dogs[J]. Veterinary Medicine(Auckland,N.Z.),2019,10:29-35.
[15]	Nai Y,Liu H,Bi X,et al. Protective effect of astaxanthin on acute cerebral infarction in rats[J]. Human & Experimental Toxicology,2018,37(9):929.
[16]	Bhuvaneswari S,Yogalakshmi B,Sreeja S,et al. Astaxanthin reduces hepatic endoplasmic reticulum stress and nuclear factor-kappa B-mediated inflammation in high fructose and high fat diet-fed mice[J]. Cell Stress Chaperones,2014,2(19):183-191.
[17]	Xu J,Gao H,Zhang L,et al. A combination of flaxseed oil and astaxanthin alleviates atherosclerosis risk factors in high fat diet fed rats[J]. Lipids in Health and Disease,2014,13:63.
[18]	江利华,柳慧芳,郝光飞. 虾青素抗氧化能力研究进展[J]. 食品工业科技,2019,40(10):350-354.
[19]	黄浦俊,朱巧巧,颜美秋,等.破壁、未破壁雨生红球藻粉体外抗氧化活性及体内抗衰老作用及比较研究[J].中药药理与临床,2018,34(2):83-87.
[20]	Zhao X Y,Zhang X W,Liu H K,et al. Enzyme-assisted extraction of astaxanthin from Haematococcus pluvialis and its stability and antioxidant activity[J]. Food Science and Biotechnology,2019,28(6):1637-1647.
[21]	杨智才.血清TBA、AST、ALT与AST/ALT联合检测在诊断急性肝炎中的应用价值[J].当代医药论丛,2019,17(15):167-168.
[22]	Yousefi M H,Dehpour A R,Ansari N S,et al. Hepatoprotective effects of standardized extracts from an ancient italian apple variety(mela rosa dei monti sibillini)against carbon tetrachloride(CCl₄)-induced hepatotoxicity in rats[J]. Molecules,2020,25(8):E1816.
[23]	费梦雪,农清清,赵惠柳,等.十项常用肝功指标对早期原发性肝癌的诊断价值[J].医学理论与实践,2019,32(8):1123-1125 ,1134.
[24]	Huang W,Wang Y,Wang J,et al. Clinical characteristics of 192 adult hemophagocytic lymphohistiocytosis[J]. Zhonghua Xue Ye Xue Za Zhi,2014,35(9):796-801.
[25]	宋娜,苏东峰,刘晓燕,等.麦冬多糖对糖尿病围绝经期大鼠血清SOD、GSH-Px、CAT、MDA水平的影响[J].东南大学学报(医学版),2019,38(6):979-984.
[26]	Haidar O,O'Neill N,Staunton C A,et al. Pro-inflammatory cytokines drive deregulation of potassium channel expression in primary synovial fibroblasts[J]. Front Physiol,2020,11:226.
[27]	Bjornstad P,Cherney D,Maahs D M. Early diabetic nephropathy in type 1 diabetes:New insights[J]. Current Opinion in Endocrinology Diabetes & Obesity,2014,21(4):279-286.
[28]	Boukhris M,Tomasello S D,Marza F,et al. Coronary heart disease in postmenopausal women with type Ⅱ diabetes mellitus and the impact of estrogen replacement therapy:A narrative review[J]. International Journal of Endocrinology,2014,2014:413920.
[29]	Bril F,Laura Millán,Kalavalpalli S,et al. Use of a metabolomic approach to non-invasively diagnose nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus[J]. Diabetes Obesity and Metabolism,2018,20(7):1702-1709.
[30]	Mansour A,Mohajeri-Tehrani M R,Samadi M,et al. Risk factors for non-alcoholic fatty liver disease-associated hepatic fibrosis in type 2 diabetes patients[J]. Acta Diabetologica,2019(3):1199-1207.
[31]	Han P,Shao M,Guo L,et al. Niclosamide ethanolamine improves diabetes and diabetic kidney disease in mice[J]. American Journal of Translational Research,2018,10(4):1071-1084.
[32]	Veron D,Reidy K J,Bertuccio C,et al. Overexpression of VEGF-A in podocytes of adult mice causes glomerular disease[J]. Kidney International,2010,77(11):989-999.
[33]	刘绛,林中军.百令胶囊联合贝前列素钠降低糖尿病肾病蛋白尿的疗效观察[J].中国实用医药,2020,15(3):101-102.
[34]	Müller-Deile J,Worthmann K,Saleem M,et al. The balance of autocrine VEGF-A and VEGF-C determines podocyte survival[J]. American Journal of Physiology Renal Physiology,2009,297(6):F1656-67.
[35]	Bhaskaragoud G,Geetha V,Sharanappa T,et al. Hypolipidemic and antioxidant properties of oryzanol concentrate in reducing diabetic nephropathy via SREBP1 downregulation rather than β-oxidation[J]. Molecular Nutrition & Food Research,2018,62(8):e1700511.
[36]	李勇超,贺青华,刘瑞雪,等.雨生红球藻源虾青素对糖尿病小鼠的降糖作用及其机制[J].食品工业科技,2016,37(24):355-359.
[37]	魏芬芬,王文娟,贺青华,等.雨生红球藻破壁孢子粉对小鼠酒精性损伤肾脏的保护作用研究[J].生命科学研究,2019,23(2):122-127.
[38]	魏芬芬,王文娟,贺青华,等.雨生红球藻破壁孢子粉对小鼠酒精性肝损伤的保护作用[J].食品工业科技,2019,40(5):270-274.
[39]	李勇超,赵健,任超,等.雨生红球藻破壁孢子粉对Ⅱ型糖尿病大鼠血糖及血脂的干预作用[J].中国食品学报,2018,18(10):31-37.