Optimization of Extraction Technology and Anti-inflammatory Activity of Total Flavonoids from Artemisia selengensis Turcz.

Objective:To optimize the technological conditions of total flavonoids extraction from Artemisia selengensis Turcz.(ATTF)and study the anti-inflammatory activity of the extracts. Methods:A quadratic regression model with the effect of compound enzyme dosage,ultrasonic temperature,ethanol volume fraction and liquid-to-solid ratio on ATTF yield were established using the central composite design(CCD)in response surface analysis. A cell inflammation model was established using lipopolysaccharide(LPS)-induced RAW264.7 macrophages to secrete nitric oxide(NO),and then different concentrations of ATTF extracts were added to determine the secretion of NO with the NO kit. Results:The optimum extraction conditions of total flavonoids from Artemisia selengensis Turcz. were the compound enzyme dosage 1.5%,ultrasonic temperature 50 ℃,ethanol volume fraction 70%,liquid-to-material ratio 40:1 (mL/g). Under these condition,the yield of ATTF was 13.09%±2.13%.The ATTF medium and high doses of the extract could significantly inhibit NO secretion in LPS-induced RAW264.7 macrophages. Conclusion:The regression model was significant,which would be used to reasonably predict the yield of ATTF. ATTF extracts would play an anti-inflammatory role by inhibiting the secretion of NO in macrophages.