LI Bing-zi, WEI Hong-yan, LEI Yun, CHEN Cheng, ZHANG Yu-bin. Mechanism of the Effect of Lactate on the Structure of Myoglobin in the in Vitro Hatching Model[J]. Science and Technology of Food Industry, 2020, 41(21): 1-7,14. DOI: 10.13386/j.issn1002-0306.2020020148
Citation: LI Bing-zi, WEI Hong-yan, LEI Yun, CHEN Cheng, ZHANG Yu-bin. Mechanism of the Effect of Lactate on the Structure of Myoglobin in the in Vitro Hatching Model[J]. Science and Technology of Food Industry, 2020, 41(21): 1-7,14. DOI: 10.13386/j.issn1002-0306.2020020148

Mechanism of the Effect of Lactate on the Structure of Myoglobin in the in Vitro Hatching Model

More Information
  • Received Date: February 16, 2020
  • Available Online: November 11, 2020
  • In order to study the interaction mechanism between lactate and myoglobin(Mb)heme prosthetic group,the longissimus dorsi was used as the raw material. Mb was separated and purified before adding calcium lactate. It was stored at 4 ℃ for 0,12,24,36,and 72 h,using ultraviolet-visible absorption spectrum,fluorescence spectrum and circular dichroism spectrum to determine the absorbance of the characteristic peak. The results showed that the absorption intensity of the ultraviolet-visible spectrum at the characteristic peak of 409 nm at four time points during refrigeration was slightly increased,but the peak position and peak shape barely changed;the intensity of the emission peak of the fluorescence spectrum at 597 nm weakened. The position of the emission peak of the porphyrin ring of Mb did not shift,but the fluorescence intensity weakened with the extension of storage time;circular dichroism chromatography showed that the shape and shoulder of the three characteristic peaks in the far ultraviolet region of 192,208 and 222 nm in the control and treatment groups The position of the peak did not change significantly,and the pattern at 190~240 nm in the calcium lactate treatment group was smooth like the control group. It shows that the covalent addition of lactate and Mb does not occur on the heme prosthetic group,but regulates myoglobin through non-heme ligand binding.
  • Related Articles

    [1]LIU Qian, JIN Wenhui, JIAO Haotian, XIE Quanling, ZHANG Yiping, HONG Zhuan, ZHAO Yuanhui. Optimization of Extraction Process and Analysis of Monosaccharide Composition of β-1,3-xylan from Caulerpa lentillifera[J]. Science and Technology of Food Industry, 2023, 44(16): 210-217. DOI: 10.13386/j.issn1002-0306.2022100086
    [2]CHEN Yang, WANG Peng, PAN Kaijin, WANG Zhe, XU Jian, ZHOU Junqiang, LIAO Ziwei. Optimization of the Extraction Process of Highland Barley β-glucan by Three-phase Partitioning and Its Molecular Weight Distribution[J]. Science and Technology of Food Industry, 2023, 44(14): 220-228. DOI: 10.13386/j.issn1002-0306.2022100064
    [3]LIU Xin-qi, HE Xian-zhe, LIU Jie-chun, TANG Qing-jiu, GU Fei-yan, YU Ling. Study on Optimization of Extraction Process of Barley Bran β-Glucan by Fermentation and Its Physicochemical Properties[J]. Science and Technology of Food Industry, 2020, 41(7): 49-54. DOI: 10.13386/j.issn1002-0306.2020.07.009
    [4]CHENG Chao, ZHANG Hong-hai, SHENG Wen-jun, HAN Shun-yu, WANG Jing. Extraction of β-Glucan from waste wine yeast by multiple cell-wall-broken technologies[J]. Science and Technology of Food Industry, 2016, (04): 111-116. DOI: 10.13386/j.issn1002-0306.2016.04.013
    [5]ZHU Xia, LI Ying, FU Wen-li, DU Na, XI Jing, HAN Shun-yu, SHENG Wen-jun, NIU Li-li, YANG Xue-shan. Process optimization of the alkaline enzymatic extracting heme from sheep blood[J]. Science and Technology of Food Industry, 2014, (18): 199-202. DOI: 10.13386/j.issn1002-0306.2014.18.034
    [6]DONG Xing-ye, SUN Chu, LIU Yao, WU Fei. Effect of ultrasonic treatment on oat β-glucan extraction and its properties[J]. Science and Technology of Food Industry, 2014, (16): 294-297. DOI: 10.13386/j.issn1002-0306.2014.16.056
    [7]桔皮中提取橙皮甙的优化工艺[J]. Science and Technology of Food Industry, 1999, (06): 33-34. DOI: 10.13386/j.issn1002-0306.1999.06.072
    [8]一品红红色素的提取及其稳定性研究[J]. Science and Technology of Food Industry, 1999, (06): 24-26. DOI: 10.13386/j.issn1002-0306.1999.06.068
    [9]大蒜油提取的比较研究[J]. Science and Technology of Food Industry, 1999, (05): 16-18. DOI: 10.13386/j.issn1002-0306.1999.05.004
    [10]黄刺玫叶片中黄酮物质的提取和分析[J]. Science and Technology of Food Industry, 1999, (05): 14-15. DOI: 10.13386/j.issn1002-0306.1999.05.003

Catalog

    Article Metrics

    Article views (353) PDF downloads (47) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return