Effect of Different Harvesting Times on Total Flavonoids Content and Antioxidant Activity in Rosae Chinensis Flos

Objective:The best harvest time was determined by comparing the content of total flavonoids and antioxidant activity of Rosae Chinensis Flos in different harvest time,which laid a theoretical foundation for the effectiveness of clinical medication of Rosae Chinensis Flos. Methods:The total flavonoids of Rosae Chinensis Flos were extracted by reflux extraction and determined by UV spectrophotometry. The antioxidant activity of Rosae Chinensis Flos was evaluated by scavenging DPPH· and OH·. Results:The change in content of total flavonoids in different harvest time was:April>May>September>October>November>June>July>August. The content of total flavonoids from rose flowers was the highest in April,which was(152.28±1.69) mg/g. The total flavonoids from Rosae Chinensis Flos in different harvesting times exhibited good antioxidant activity. Among them,the best antioxidant activity was observed in total flavonoids from Rosae Chinensis Flos harvested in July. Conclusion:There was positive correlation between the content of total flavonoids and antioxidant activity in Rosae Chinensis Flos. However,its antioxidant activities were affected by harvesting time and other impurities.