Isolation,Identification and Flocculation Characteristics of a Pseudomonas Strain

Objective:In order to obtain high efficiency and low cost microbial flocculant.Methods:Using conventional screening techniques to isolate and screen strains from the sewage sludge along the Longjiang River in Yizhou. The identification of the strains was carried out through morphological observation,physical and chemical analysis and molecular level 16S rDNA systematic evolution analysis;according to conventional methods,the flocculant was investigated in kaolin. The flocculation performance in activated carbon and yeast suspension and the factors affecting flocculation performance.Results:L2019-2 was identified as Pseudomonas veronii. The flocculant produced by L2019-2 had flocculant activity in both inorganic and organic suspensions.The flocculant’s flocculant activity was influenced by cation,pH,temperature and other factors. When the pH was 5.0,the temperature was 30 ℃ and the coagulant aid was Fe³⁺,the flocculant had the optimum flocculating activity in kaolin suspension. After chemical composition analysis,the flocculant was acid glycoprotein.Conclusion:The flocculant produced by L2019-2 has good flocculating activity,which can provide good strain resources for the study of microbial flocculant,and has better application potential in sewage treatment.