Optimization of Enzymatic Extraction Technology of Starch from Breadfruit by Response Surface Methodology

In order to determine the optimum technological conditions of enzymatic extraction of breadfruit starch,neutral protease method was used to extract breadfruit starch. The effects of four factors,such as solid-liquid ratio,enzymatic hydrolysis temperature,enzymatic hydrolysis time and amount of enzyme,on the extraction rate of bread fruit starch were studied.On this basis,the response surface methodology was used to optimize the extraction technology of breadfruit starch. The results showed that the optimal combinations of extraction process for maximum breadfruit starch production were determined as solid-liquid ratio of 1:4 g/mL,enzymatic hydrolysis temperature of 62 ℃,enzymatic hydrolysis time of 6 h,and amount of enzyme of 0.13%,respectively. Under the best conditions,breadfruit starch extraction rate of the theoretical value was 69.66%,the actual value was 69.97%,the fitting model was in accordance with the actual verification. Neutral protease method is an efficient method for the extraction of breadfruit starch,which has the potential for industrial extraction of breadfruit starch.