pH Responded Colorimetric ELISA for Sensitive Detection of <i>Salmonella Choleraesuis</i> in Milk

LI Qian-ying; ZHANG Kang-kang; ZHOU Yao-feng; HUANG Xiao-lin; LI Xiang-min; XIONG Yong-hua

doi:10.13386/j.issn1002-0306.2019100100

Volume 42 Issue 1

Dec. 2020

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Science and Technology of Food Industry > 2021 > 42(1): 227-232. > DOI: 10.13386/j.issn1002-0306.2019100100

LI Qian-ying, ZHANG Kang-kang, ZHOU Yao-feng, HUANG Xiao-lin, LI Xiang-min, XIONG Yong-hua. pH Responded Colorimetric ELISA for Sensitive Detection of Salmonella Choleraesuis in Milk[J]. Science and Technology of Food Industry, 2021, 42(1): 227-232. DOI: 10.13386/j.issn1002-0306.2019100100

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pH Responded Colorimetric ELISA for Sensitive Detection of Salmonella Choleraesuis in Milk

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
2. School of Food Science and Technology, Nanchang University, Nanchang 330031, China

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Received Date: October 15, 2019
Available Online: January 07, 2021

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Abstract

Abstract

Human maybe get poisoning after eating Salmonella choleraesuis-infected food. Thus,developing a new analytical technology for rapid and sensitive detection of Salmonella choleraesuis in food is urgently needed. Hence,a pH responded colorimetric enzyme-linked immunosorbent assay(pH-ELISA)was developed for sensitive detection of Salmonella choleraesuis in milk. In this method,glucose oxidase(GOx)was used to catalyze glucose for the generation of gluconic acid. The color of bromocresol purple(BCP)was changed from purple to bright yellow because the pH of sample solution decreased. Under the optimal conditions,the proposed method achieved two linear independent regression equations. When Salmonella choleraesuis concentration increased from 2.54×10³ to 6.17×10⁵ CFU/mL,the first regression equation was described as y₁=0.1051ln(x)+0.7024(R²=0.7513). With the increase of Salmonella choleraesuis concentration from 6.17×10⁵ to 1.67×10⁷ CFU/mL,the second regression equation was expressed as y₂=1.3216ln(x)-15.797(R²=0.9711). The average recoveries of the proposed method for spiked milk samples at Salmonella choleraesuis concentrations ranging from 2.5×10³~5.6×10⁶ CFU/mL were 72.16%~103.58% with the relative standard deviations ranging from 7.54%~15.30%. In conclusion,the developed colorimetric ELISA is suitable for the rapid and sensitive quantitation of Salmonella choleraesuis in milk.

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